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Query: UMLS:C1275122 (TEM)
 21,810 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In a study of lymphocyte subpopulations in peripheral blood during infectious mononucleosis the lymphocytosis was found to be of T-cell origin (i.e. E-RFC), while the number of non T lymphocytes (i.e. EA-, EAC-RFC and SmIg + ve cells) was normal in 7 out of 8 patients. Ten patients were tested for the presence of HLA-DR determinants on their B and T cells and not only B lymphocytes but also a great part (31--75%) of T cells were lysed by the anti HLA-DR testsera, indicating that HLA-DR determinants are expressed on a population of T cells in IM patients. After recovery all patients were retested and showed a normal pattern of HLA-DR typing. This indicates an increase of HLA-DR antigens on T cells or a vigorous proliferation of a small DR-positive T-cell subpopulation during the acute stage of IM.
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PMID:Lymphocyte subpopulations in man. Expression of HLA-DR determinants on human T cells in infectious mononucleosis. 8 94

The survival time of skin allografts from RhLA-nonidentical, unrelated donors was increased from a mean of 7.69 days in controls (n = 20) to a mean of 32.53 days in rhesus monkeys (n = 21) receiving a total dose of 250 mg of rabbit anti-human thymocyte globulin (RATG) per kg. Immunological monitoring studies were performed on the peripheral blood of mononuclear cells in control and treated monkeys. After administration of RATG, the percentage of E rosette-forming cells (E-RFC) was greater than 90% depressed, and the percentage of EAC rosette-forming cells was increased 5-fold in the circulation. Significant numbers of RATG-coated cells were detected only during the first week after RATG treatment. The percentage of E-RFC recovered to pretreatment levels within 3 to 4 weeks after RATG treatment, although the absolute E-RFC count remained depressed for 2 to 3 months. In addition, the in vitro proliferative responsiveness to polyclonal mitogens and to allogeneic lymphocytes remained greater than 80% depressed for 2 to 3 months after RATG treatment. The incidence of post-transplant-specific antidonor lymphocyte-mediated cytotoxicity (LMC) was similar in controls (85%) and RATG-treated monkeys (81%), and the appearance of LMC was correlated (r = 0.711) with partial recovery of absolute ERFC counts in the treated group. The appearance and peak of LMC were delayed (P less than 0.001) in RATG-treated monkeys, but preceded and correlated with rejection. Prior to rejection, the serum of RATG-treated monkeys inhibited LMC. Antibody-dependent cellular cytotoxicity appeared after rejection in the majority of recipients in both groups. The appearance and peak of antibody-dependent cell-mediated cytotoxicity (ADCC) were delayed (P less than than 0.001) in RATG-treated monkeys, but did not exhibit a significant correlation with the time of rejection.
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PMID:Effect of rabbit anti-human thymocyte globulin on lymphocyte subpopulations and functions following allotransplantation in the rhesus monkey. 10 31

Subpopulations of human peripheral blood leukocytes were isolated by rosette formation and tested for functional activity. E -rosette-forming cells (E-RFC) and EAC-RFC were separated from non-resetting cells by sedimentation on Ficoll-Hypaque gradients. The efficiency of the method and the purity of the resulting subpopulations were high. E-RFC responded to PHA Con A, allogeneic leukocytes, and PPD, with higher levels of proliferative reactivity than the unseparated lymphocytes while E-RFC depleted, EAC-RFC, and null cells showed only low levels of reactivity. Reactivity to PWM and tetanus toxoid was also restricted to the E-RFC subpopulation, but was lower than that of unseparated cells. A staphylococcal antigen preparation triggered lymphoproliferative reactivity in the E-RFC, E-RFC depleted, EAC-RFC, and the null cell subpopulations. 51Cr release lymphocyte cytotoxicity against a human lymphoblast target cell line was found in the E-RFC and null cell fractions but was not observed with the EAC-RFC subpopulation.
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PMID:Functional activities of rosette separated human peripheral blood leukocytes. 12 61

Cells bearing surface immunoglobulins (Ig+-cells) detected by the indirect immunofluorescent method and cells forming rosettes (RFC) with sheep erythrocytes coated with antibody and complement (EAC rosettes) were found in the liver and the spleen on the 15th and the 20th days of prenatal life of rats. The percentage of Ig+-cells and RFC in the liver was high and remained unchanged and at about the same level during the whole postnatal life. The spleen and the bone marrow displayed an increase of the Ig+-cells and RFC increased throughout the 1st month of postnatal life with the maximum at the 30th day after birth; a sharp decrease occurred in old animals. In the thymus the Ig+-cells and the RFC were either absent or present in very small amounts only at some periods of study. Ig+-cells with "capping" were discovered in the spleen and bone marrow on the 5th--10th days of postnatal life; their count increased considerably in 30-day and adult rats. Such cells were absent in the lymphoid tissues of old 40-month rats.
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PMID:[Properties and kinetics of a population of B-lymphocytes during rat ontogenesis]. 30 26

Fluctuations in the percentages and absolute numbers of T and B lymphocytes were observed in the peripheral blood of a patient with severe combined immunodeficiency maintained in a gnotobiotic environment. Up to 24 months of age, 72-86% of the lymphocytes had surface membrane immunoglobulin (SMIg), 37-47% bore a receptor for C3(EAC-RFC), and 3-12.5% formed spontaneous rosettes with sheep erythrocytes (E-RFC). These values persisted until 30 months, after which shifts in the percentages and absolute numbers of T and B cells were observed. A significant decrease in the proportion of SMIg-bearing cells to 20-40% (169-405 mm3), and EAC-RFC to 10.5-39% (114-259 mm3), was accompanied by a general increase in the proportion of T cells to 19-60% (141-1026 mm3), representing a lymphoid subpopulation approach to normal levels.
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PMID:A longitudinal study of T and B lymphocytes from a three-year-old patient with severe combined immunodeficiency (SCID) in 'gnotobiotic protection'. 30 11

The percentages and absolute numbers of T, B and "null" cells were studied in 100 allergic patients and compared with values observed in 35 healthy age matched control subjects. T cells were identified by their ability to form spontaneous rosettes with SRBC (E-RFC) and B cells by their complement receptor that allows the binding of antibody-complement coated erythrocytes (EAC-RFC). The PBL count was found to be slightly lowered in patients suffering from pollinosis, candidiasis and chronic urticaria. The percentages of T and B lymphocytes were similar in both allergic and healthy people, but the patients with pollinosis, candidiasis and chronic urticaria showed decreased absolute numbers of T lymphocytes. Most of them had normal or even--in some cases of pollinosis and candidiasis--increased numbers of B lymphocytes, and normal or--as in some cases of chronic urticaria and candidiasis--increased numbers of B lymphocytes, and normal or--as in some cases of chronic urticaria and candidiasis--decreased numbers of "null" cells. This study support the hypothesis that allergic diseases may be associated with a defect of a subpopulation of T cells, however, it does not seem possible that "null" cells may represent these defective suppressor T cells.
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PMID:T and B lymphocytes in various allergies. 30

Guinea pig lymph node cells suspension (LNC-O) was filtered through a glass wool column and the effluent (LNC-G) was further filtered through a nylon column. In this effluent (LNC-NE) about 30 per cent of the lymphocytes was identified as non-rosette forming cells (non-RFC). The non-RFC fraction was separated from LNC-NE fraction by Ficol-Conray specific gravity centrifugation or effluent cells reacted previously to rabbit red blood cells (RRBC). The upper layer after centrifugation, designated non-RFC fraction, was separated. In this fraction 96% of the cells were lymphocytes and about 95% of them were non-RFC, which lacked receptors for rabbit red blood cells (RRBC) or EAC and detectable surface immunoglobulin by conventional techniques. Though the response of the lymphocytes in the non-RFC fraction to mitogenic (Con-A, LPS) or antigenic stimulation was lower in comparison with that in RFC-rich fraction, the response of non-RFC to ConA exceeded the response to LPS. These facts suggest that at least a portion of the non-RFC may be cells from the T-cell line.
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PMID:Separation of RRBC-RFC and non-rosette forming cells (non-RFC) of guinea pig T-cell populations and their functional difference. 1. Response of RRBC-RFC and non-RFC to either Con-A or LPS. 31 Sep 44

Comparative studies on cord and adult blood showed that cord blood contained at least twice as many lymphocytes as adult blood. Relatively, the percentage of T cells (E-RFC) was significantly lower in cord blood lymphocytes. The percentage of B cells (EAC-RFC and SmIg bearing cells), as well as the total number of T and B cells (mm(-3)), was significantly higher in cord blood. In vitro mitogen transformation of cord and adult lymphocytes in while blood, cultured for different times and diluted to contain equivalent numbers of lymphocytes per culture, showed significant qualitative and quantitative differences. Responses to the T cell mitogens phytohaemagglutinin (PHA) and concanavalin A (Con A) were examined from 3 to 6 days in culture. Cord blood lymphocytes were significantly more responsive when cultured for 3 to 4 days, similar to adult cells after 5 days, but significantly less responsive after 6 days in culture. The optimal levels of T cell mitogen responsiveness in cord cells (Day 4) were similar to adut cells (Day 6). Spontaneous transformation of unstimulated lymphocytes and B cell mitogen transformation with pokeweed mitogen (PWM) and staphylococcal protein A (SpA) were all significantly higher in cord blood than in adult whole blood cultured for 5 days.
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PMID:Cord blood lymphocyte subpopulations and mitogenic activity in whole blood microculture. 31 31

Peripheral leucocyte migration inhibition (LMI) with Trypanosoma cruzi-specific antigens, measured as a migration index (MI), was studied in chronic Chagas' disease patients. The MI of untreated patients with polymerized antigens from culture forms (epimastigotes) of T. cruzi was significantly lower than that of controls. In contrast, when chronic Chagas' patients were treated with nifurtimox, 10 mg/kg/day for 2 months, the MI was not different from control values. Treated and untreated patients had normal T- and B-lymphocyte markers, measured by the ability to form rosettes either with sheep erythrocytes (E-RFC) or with sheep erythrocytes--antibody--complement (EAC-RFC). In addition, the number of lymphocytes bearing surface membrane Ig (SMIg) was the same as that of controls. Non-specific functional assays, such as PHA-induced blastogenesis and antibody-dependent cell-mediated cytotoxicity (ADCC) to sensitized chicken erythrocytes were also normal, both in treated and untreated patients. Thus, nifurtimox produced a particularly effect on cell-mediated immunity, specially detectable using LMI.
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PMID:Cell-mediated immunity in Chagas' disease: Alterations induced by treatment with a trypanocidal drug (nifurtimox). 41 67

After vaccination of five volunteers with yellow fever live vaccine, blood mononuclear cells were isolated and labelled with 3H-thymidine at intervals. DNA synthesis was measured by scintillation counting and autoradiography of rosetted cells. Rosetting with sheep erythrocytes (E-RFC) identified T cells, and such erythrocytes coated with IgM antibodies and complement (EAC-RFC) identified B cells and monocytes. DNA synthesis in the total mononuclear cell fraction, as well as in subfractions enriched in or deprived of E-RFC, displayed a sharp increase on day 10--11 after vaccination, remained high on day 13--14, and then returned to the prevaccination level. There was a corresponding morphological transformation, measured by size distribution and number of nucleoli per cell. The major fraction of DNA-synthesizing cells before, during and after the peak of activity was found among non-rosette-forming cells. However, during the activity peak the numbers and proportion of DNA-synthesizing E-RFC were increased while the response with regard to EAC-RFC was not obvious. Thus within a complex cellular response a transient T-cell response was identified.
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PMID:DNA synthesis in subpopulations of blood mononuclear leucocytes in human subjects after vaccination against yellow fever. 71 79


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