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Query: UMLS:C1275122 (TEM)
 21,810 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A rabbit antiserum to human fetal brain after multiple absorption reacted with 100% of thymocytes, 55% of peripheral blood lymphocytes and 90% of enriched T lymphocytes, but not significantly with B lymphocytes. Spontaneous SRBC rosette formation was inhibited by anti-BAT pretreatment, but EAC-rosette formation remained unaffected. The antiserum was itself highly stimulatory. However, cells treated with the antiserum and complement exhibited marked inhibition of responsiveness to Con A, little effect with PHA and no alteration with PWM. The MLC reaction was inhibited only when the responder cells were treated with the antiserum and complement. Treatment of sensitized lymphocytes with the antiserum and complement caused a dose-dependent suppression of blastogenic response to both PPD and n-DNA. No effect, however, was noted in MIF producing cells. Con A induced suppressor function of lymphocytes was abolished by treatment with the antiserum and complement. These results indicate that the anti-BAT serum obtained by us can be utilized for the isolation of T lymphocyte subsets.
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PMID:Characteristic and functional specificity of anti-human BAT (brain associated thymocyte antigen) serum. 616 42

Selected parametres reflecting cellular and humoral immunity were analyzed in 98, 10 year-old children, who had been hospitalized during first 2 years of life because of bronchitis. In this group, 46 children suffered from obstructive bronchitis and 52 from other types of bronchitis. Serum immunoglobulins, complement components, and antiproteolytic factor levels, T and B lymphocytes counts, leukocyte migration inhibition, and skin tests to PHA and Polyvaccine were compared and analyzed in relation to bronchial obstruction and allergic symptoms in early childhood. The results obtained did not show any significant difference when the group with obstructive type of bronchitis was compared to the rest of children studied. Some differences (increase of the percentage of EAC-FRC and decrease of the levels of the antiproteolytic factors) were noted in relation to allergic symptoms.
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PMID:The evaluation of immune status in children with the past history of obstructive bronchitis. 616 21

The effect of two specific placental proteins, trophoblastic beta 1-glycoprotein (TBG) and chorionic alpha 1-microglobulin (CAG), on the immunological reactions was studied in vitro. TBG in physiological doses suppressed the proliferation of lymphocytes induced by plant mitogens and allogenic cells in the unidirectional mixed cultures, strengthened the effect of concanavalin A upon the induction of cells-suppressors in the culture and, in low concentrations, decreased the percentage of E- and EAC-rosette-forming cells. In none of the tests used CAG was effective. But when studying the effect of TBG and CAG mixture on PHA-induced proliferation of lymphocytes the inhibiting effect of TBG was weakened and, in some cases, completely relieved.
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PMID:[Placental proteins as regulators of immunological reactions in pregnancy]. 618 44

Results of the PHA skin test correlate with prognosis of cancer patients (W. Wiktor-Jedrzejczak [17] ). However, its clinical interpretation is faded by not known character of this reaction. In the present study PHA skin test was performed on 50 subjects simultaneously with: 1. sealed capillary migration inhibition test in the presence of various concentrations of PHA; 2. E and EAC rosette tests for quantitation of T and B lymphocytes. Skin reaction to 1 microgram of purified PHA (Wellcome) was found to correlate with migration inhibition performed using suboptimal doses of PHA-P (Difco). -No correlation was found between leukocyte and lymphocyte absolute count and PHA skin test. Neither proportions nor absolute numbers of E+, and EAC+ and null cells correlated with PHA skin test. On several occasions the test persisted positive despite absence of lymphocytes in peripheral blood of patients. -These studies further suggest that PHA skin test has certain characteristics similar to DTH reactions and that changes of the test do not simply reflect changes in PBL subpopulations.
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PMID:PHA skin test-correlation with migration inhibition but not with peripheral blood lymphocytes (PBL) subpopulations. With 1 figure. 621 37

T lymphocytes were isolated from monocyte-depleted mononuclear cells of normal individuals by rosetting them with sheep erythrocytes. These purified T cells were preferentially depleted of cells with receptors for FcG (TG cells), FcM (TM cells), or C'3 (TC cells) by rosette formation with EA(G), EA(M), and EAC, respectively, before or after incubation for 24 hr in medium 199 fortified with fetal calf serum (20%). The unfractionated lymphocytes and the purified and the depleted T cells were analyzed for receptors to FcG, FcM, and C'3 and for cytotoxic activity in the natural killer (NK), antibody dependent cell-mediated cytotoxicity (ADCC), and mitogen-induced cell-mediated cytotoxicity (MICC) assays. The TG and TC cells were detected among the freshly isolated T cells, whereas the TM cells were detected only following 24 hr of incubation. Removal of TC cells from the 24-hr-cultured T cells resulted in removal of all the TC cells and in the concomitant removal of the majority of TM cells. Similarly, removal of TM cells from the 24-hr-cultured T cells resulted in the elimination of all TM cells as well as the majority of TC cells. These results demonstrate the in vitro generation of T cells with receptors for both FcM and C'3 (TM+C cells). Ten percent of the freshly isolated TG cells possessed detectable receptors for C'3 and/or FcM. These cells constitute the TG+C and TG+M lymphocytes. Support for consideration of these receptor-bearing cells as unique and stable cells is provided by the finding that TM and TC cells maintained in culture for up to 72 hr do not generate other receptors but retain the single receptor which characterizes each of these cells. Only a small percentage of cultured TG cells generate receptors for C'3 and FcM. It may therefore be concluded that the TG, TM, and TC cells are stable unireceptor-bearing cells. The TG, TM, TC, TG+C, TG+M, and TM+C lymphocytes account for approximately 50% of the circulating lymphocytes. Whether the remaining cells, the T null or TN cells, constitute the precursors for any or all of the receptor-bearing T cells remains to be determined. Unfractionated freshly isolated T cells were highly cytotoxic in the NK and PWM-mediated MICC assays but were relatively inactive in the ADCC, naturally occurring cell-mediated cytotoxicity (NOCC), and PHA- and Con-A-mediated MICC assays.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Surface receptors and immune activity of purified human circulating mononuclear cells. IV. The demonstration of seven subclasses of T cells in the circulation of the normal individual: the cytotoxic activities of these cells. 622 1

In this study enzyme activities involved in purine metabolism were measured in T and B lymphocytes separated by E and EAC rosetting methods. Adenosine deaminase, purine nucleoside phosphorylase and HGPRTase activities were significantly elevated in T cells, compared to the activities in B cells. There were no significant differences in adenosine kinase and APRTase activities between T and B lymphocytes. In contrast, PRPPsynthetase activities were higher in B cells than in T cells. The uptake of various radiolabeled precursors by mitogen stimulated lymphocytes was studied. The uptake of 14C-formate by the mitogen stimulated lymphocytes was markedly lower, compared to that of 14C-adenosine and or 14C-purine bases. The uptake of 14C-adenosine by PHA stimulated lymphocytes was considerably higher than that of Con A or PWM stimulated lymphocytes. However, the uptake of 14C-hypoxanthine into lymphocytes stimulated with PWM was increased by comparison with unstimulated lymphocytes. From these results it seems that adenosine plays a central role in the metabolism of T cells, and that purine bases are preferentially utilized in B cells.
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PMID:The differences in purine metabolism between T and B lymphocytes. 625 35

Thirty-two cases of hydatidiform mole without endometrial invasion of chorial tissue and 17 of invasive mole were studied to ascertain whether these two diseases can be differentiated by various immune parameters. All of hydatidiform 11 of invasive mole were sampled at a state of "mole in utero" (pre-evacuation), but 6 of invasive mole were at a post-evacuation state, respectively. The results were summarized in the following items; 1. Serum total protein, especially albumin decreased with no significant difference in both groups. Globulin fractionation (alpha 1, alpha 2, beta, gamma-globulin) showed no characteristic changes. 2. A variation of immunoglobin (IgG, IgM, IgA) was similar in both. 3. Beta 1 C (a component of complement) increased in two groups, but no difference was revealed. 4. Leucocytosis was observed, but lymphocytosis was not detected in both groups. 5. Active E-RFC slightly increased, although total E-RFC and EAC-RFC were within normal range. But these findings were not statistically different in both. 6. In vitro lymphocyte-PHA-response was fairly depressed without a significant difference in two groups. 7. Suppressive effect of serum on lymphocyte-response was also slightly strengthened, but a difference in both was not significant. 8. The results of immune skin tests (PHA, PPD, SK-SD, Candida) could not be debated with liquid numbers studied.
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PMID:[Immunological analysis of hydatidiform mole and invasive mole (author's transl)]. 626 1

The effect of postoperative irradiation on the immune functions of 13 patients with breast carcinoma is reported, using as parameters the peripheral blood lymphocyte count, percentages of E and EAC rosette forming cells, and lymphocyte proliferative responses to PHA, Con A and PPD. After irradiation the number of peripheral blood lymphocytes was reduced during 8 months. The percentages of E and EAC rosette forming cells were not altered during the observation time of 7 to 36 months. In the proliferative responses of lymphocytes to PHA, Con A and PPD, the postoperative irradiation caused a decrease which, regarding PHA and Con A, lasted up to 8 months and then recovered. The decrease in the proliferative responses to PPD was stronger and lasted during the whole observation time. In the mitogenic responses of patients with recurrent or disseminating disease no difference could be demonstrated as compared with the patients living recurrence-free.
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PMID:Long-term effect of postoperative irradiation on the immune functions in patients with mammary carcinoma. 627 Sep 63

The paper is a continuation of the previous experiments. The results show that the rabbit and sheep antisera specific for monocytes after absorption with leukocytes deprived of phagocytosing cells do not affect E-rosetting cells and do not impair the blast transformation of normal leukocytes stimulated by PHA in vitro. However, they reduce the number of EAC rosettes formed by leukocytes and they decrease the ability of leukocytes to produce antibodies for SRBC.
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PMID:Antimonocyte serum. The effect on blast transformation, RFC and PFC formation. 636 99

27 cases of ornithosis were observed during an epidemia in 1980 in Kielce and subsequently followed with respect to immunological characteristics of peripheral blood lymphocytes. Blastic transformation of these cells was tested after stimulation in vitro with three different mitogens. Identification of peripheral blood T and B lymphocytes was done using rosette tests (E,EA,EAC) and the occurrence of surface immunoglobulins was determined by the immunofluorescent method with polyvalent anti-immunoglobulin serum. The counts of T and B lymphocytes in the peripheral blood were normal throughout the whole period of the observation, but from the 3rd week on a significant impairment of 3H-thymidine incorporation into the cells stimulated with Con A was observed, and from the 10th week on, this impairment appeared also in cells stimulated with PHA and PWM. These observations revealed considerable disturbances in cell-mediated reactivity in patients with ornithosis and seem to be connected with chronic infection with Chlamydia psittaci.
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PMID:Disturbances of cell-mediated immunity in ornithosis. 638 19


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