Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1275122 (TEM)
 21,810 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using peripheral blood lymphocytes separated by a Ficoll method and suspended in saline, means of 77-1% (s.d. 5-2) E rosettes (T lymphocytes) and 20-1% (s.d. 6-7) EAC rosettes (B lymphocytes) have been obtained with normal healthy donors. Poorer E-rosette formation resulted from higher centrifugation speeds during the washing of lymphocytes or erythrocytes, insufficient chilling, or rough handling. The presence of 5% albumin in the final mixture stabilized the rosettes and brought a constant subpopulation of B lymphocytes into rosetting. In patients with bronchial carcinoma who, at the time of diagnosis, had E-rosette percentages below 1 s.d. of the mean for normal donors, the length of survival was significantly shorter than in those with normal or high values. The same was true for those in whom null cells were detected. In each case the correlation effect was mainly found in the group of patients with squamous carcinoma.
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PMID:E and EAC rosetting lymphocytes in patients with carcinoma of bronchus I. Some parameters of the test and of its prognostic significance. 17 86

A system for screening fungal metabolites with cytotoxic activity against tumor cells is described and the results obtained using this system are discussed. It was found that 35.2 per cent of the strains isolated from uranium mines had a cytotoxic effect on the EAC cells in vitro. As for the strains isolated from other sources only 6.85 and 9.87 per cent of them inhibited the EAC cells in vitro. Five substances, i. e. vermiculline, PSX-I, Frequentine, bikaverin and duclauxin isolated from 227 evaluated cultures showed a strong inhibitory effect on the EAC cells and other tumors in vitro.
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PMID:[Screening of antitumor antibiotics, formed by molds]. 17 70

To investigate the nature of non-endemic Burkitt's lymphoma, we examined neoplastic cells from eight American patients for receptors for sheep erythrocytes (E), complement (EAC), and Fc fragment of lgG (igGEA), and for surface immunoglobulins (Slg) and hydrolytic enzymes. In addition, we reviewed 47 biopsies and 17 autopsies from American patients to ascertain patterns of involvement by tumor in lymph nodes, spleens and Peyer's patches. Neoplastic cells in all cases studies bore monoclonal surface immunoglobulins of the igM class. Receptors for EAC and igGEA were identified on a minority of the cells. Little or no hydrolytic enzyme activity was demonstrable. These results indicate that, like Burkitt's lymphoma in Africans, this histologically identical tumor in American patients consists of B lymphocytes. In 10 biopsies and two autopsies, germinal centers were selectively involved by tumor, suggesting that these neoplastic cells may be related to some B lymphocytes of normal germinal centers.
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PMID:Non-endemic Burkitts's lymphoma. A B-cell tumor related to germinal centers. 18 12

Depressed ADCC activity was found in sarcoidosis patients in clinical Stage II when whole blood was used as the effector cell pool. Whole blood in Stage I patients as well as purified peripheral lymphocytes of sarcoidosis patients did not reveal a diminished cytotoxic activity. Stimulation experiments with PHA, Con A, and PPD in two different concentrations resulted in a normal PHA response, a significantly decreased Con A response (regardless of the clinical stage of the patients), and a significantly decreased PPD responsiveness of peripheral lymphocytes in Stage II patients, respectively. Regarding the distribution of peripheral B and T lymphocytes, only a significantly depressed T-cell number in Stage I sarcoidosis patients was observed. Peripheral cells forming EA and EAC rosettes and staining for membrane-bound immunoglobulins were within normal ranges. Serum antibody titers to different herpes viruses, including Epstein-Barr virus, were found not to be elevated. Twenty percent of sarcoidosis patients showed anti-immunoglobulins in their sera specific for the Fc and Fab fragment.
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PMID:Estimation of T- and K-cell activity in the peripheral blood of sarcoidosis patients. 18 96

The isolation and establishment in vitro of a hitherto undescribed type of lymphocyte designated D.G.-75 is reported. The original inoculum was derived from the pleural effusion of a child with a primary abdominal lymphoma, which clinically and histologically resembled Burkitt's lymphoma. In addition to the absence of the EBV genome and EBV receptors, this line possesses a number of other properties which distinguish it from previously described lymphoblastoid cell lines. It has different growth characteristics and morphology; does not form EAC or E rosettes (representative of B and T) cell surface markers, respectively); possesses IgM-kappa immunoglobulins on the cell surface (B lymphocyte), has an unusually high cap-forming ability and low agglutinability with fluorescent concanavalin A. One homologue of the No.14 chromosome pair possesses extra chromatin material as revealed on chromosome banding. This abnormal chromosome marker is similar to that described in biopsies and cultured tumor cells from patients with African Burkitt's lymphoma.
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PMID:Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). 18 69

Experiments were performed to analyze the characteristics of the effector cells involved in cell-mediated killing of herpes-infected fibroblasts. Effector cells were fractionated according to adherence and phagocytic properties, and lymphocytes were depleted of T-cells, and EA- and EAC- (antibody-coated bovine erythrocytes and bovine erythrocytes coated with rabbit anti-bovine antibodies and C5-deficient mouse serum, respectively) rosette-forming cells by differential centrifugation. Column passage was used as an alternative to depletion of B-cells and F(c)-receptor-positive cells. Antibody-dependent, cell-mediated cytotoxicity was found to be associated with nonphagocytic cells carrying low-avidity F(c) receptors. Some of these had surface immunoglobulin; others lacked both B- and T-cell markers. Low levels of killing were found without addition of anti-herpes antibody to the test system, and this killing seemed to depend on cells belonging to the same subpopulation as the antibody-dependent effector cells.
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PMID:Effector cell involved in cell-mediated cytotoxicity to cells infected with herpes simplex virus type 1. 19 39

The effect of acute and prolonged three week administration of prednisolone and ACTH on the numbers and function of T- and B-lymphocyte subpopulations in patients requiring corticosteroid therapy was studied. Prednisolone caused severe reduction in E-rosette-forming lymphocytes, phytohaemagglutinin response, EAC-rosette-forming lymphocytes and surface-membrane mu-positive B-lymphocytes maximal at 4--6 hr after administration with reversal sometimes to supernormal levels by 24 hr. Prolonged administration resulted in a similar pattern of response. Acute but not prolonged prednisolone administration caused a reduction in the percentage of E-rosette-forming lymphocytes maximal at 4 hr. ACTH caused moderate reduction in these parameters at 4 and 6 hr which remained low at 24 hr after prolonged administration.
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PMID:The effect of acute and prolonged administration of prednisolone and ACTH on lymphocyte subpopulations. 19 99

Eighteen patients with nasopharyngeal carcinoma (NPC) were compared to matched controls, before or after cobalt therapy, for the ability of their peripheral blood lymphocytes to: (1) form E and EAC rosettes and (2) mount a proliferative response with PHA, Con A and ALG. A slight decrease in the percentage of E rosettes and a moderate hyporesponsiveness to PHA and Con A were observed before treatment. The statistical significance of these alterations was borderline. Within the group of treated patients a much greater depression, including the response to ALG, was found, although a few long-term survivors responded to mitogens as well as the controls. These findings stress the difficulty of interpreting the results of a longitudinal study of cell-mediated immunity, specific or non-specific, in cancer patients. Finally, by comparing the proliferative response to the three mitogens before and after radiotherapy, it is suggested that their differential effect on these responses might be used in man, as it was in mice, to delineate lymphocyte subpopulations.
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PMID:Lymphocyte subpopulations and mitogenic responses in nasopharyngeal carcinoma, prior to and after radiotherapy. 20 May 72

The effect of halothane on rosette formation and cyclic adenosine monophosphate (cyclic AMP) level of human lymphocytes was investigated. A 4- or 24-hour incubation with 2% halothane had no significant effect on "early" or "late" E, EA and EAC rosette formation or intracellular cyclic AMP levels of human lymphocytes whereas cytochalasin B, which affects microfilament action, reversibly inhibited both E and EA but not EAC rosette formation. It is suggested that halothane in clinical concentrations neither disturbs the function of microfilaments, essential for E and EA rosette formation, nor affects the metabolism of cyclic AMP in unstimulated human lymphocytes.
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PMID:Lack of effect of halothane on E, EA, and EAC rosette formation and cyclic AMP level of human lymphocytes. 21 Mar 35

Forty-five nervous system tumors (9 glioblastomas, 9 meningiomas, 15 assorted primary neural tumors including 3 medulloblastomas, and 12 brain tumors metastatic to the brain were analyzed for their content of lymphocytes, granulocytes, and macrophages. Cell suspensions were prepared by enzymatic digestion; lymphocytes and granulocytes were quantitated by morphology following cytocentrifugation, and macrophages were quantitated by IgG EAC (erythrocyte-antibody-complement) rosette formation. EA (erythrocyte-antibody) adsorption to sections of tumor was employed to determine the distribution of the IgG Fc receptor-positive cells within the tumors and to serve as quality control for selective release of Fc receptor-positive cells by enzyme digestion. The 9 glioblastomas had a mean macrophage content of 41% (range: 5-78%); the 9 meningiomas, 42% (range: 5-80%); the 3 medulloblastomas, 6% (range: 2-15%); and the metastatic tumors, 21% (range: 2-50%). Lymphocyte contents were variable but generally less than 10%. Most tumors contained less than 10% granulocytes. EA adsorption demonstrated that Fc receptor-positive cells were distributed throughout the tumor mass, although different types of patterns were observed. There was an excellent correlation between the percent EAC rosette-positive cells in suspensions and the extent of EA adsorption to the tumor sections. The significance of the study primarily rests in the demonstration that most nervous system tumors contain high numbers of infiltrating host cells, primarily macrophages.
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PMID:Immunohistologic evaluation of the lymphoreticular infiltrate of human central nervous system tumors. 21 40


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