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Query: UMLS:C1275122 (TEM)
 21,810 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We characterized a cell line established from bone marrow cells from a child with acute lymphoblastic leukemia. This cell line, TC78, had lymphoblastic morphology and was cytoplasmic peroxidase and esterase negative. The cells did not have T- or B-cell properties such as E- or EAC-rosette forming ability, reactivity with monoclonal T-cell or B2 antibodies, or immunoglobulin synthesis. We concluded that TC78 was a pre-pre B-cell line based on the following monoclonal antibody staining pattern: BA-1+, BA-2+, cALLa+, Ia+, 2H7+ and OKB2+. Growth in 'Dickie' culture and reactivity with 1G10 myeloid antibody suggested coexpression of lymphoid and myeloid characteristics. However, 1G10 expression proved dependent on culture conditions, illustrating one caveat in application of monoclonal antibodies in lineage determination.
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PMID:Immunologic, morphologic and chromosomal characterization of a cell line (TC78) established from a child with acute lymphoblastic leukemia. 386 23

A fucose binding protein was detected in boar spermatozoa by means of a specifically developed modified enzyme-linked-lectin-assay using glycosylated peroxidase derivatives. The distribution of the fucose binding protein was assessed by means of fluorescence microscopy with fluoresceinyl-glycosylated peroxidase. Fucose binding was particularly prominent at the apical region of the sperm head. In order to gain more insight into the precise localization of the carbohydrate binding protein electron microscopical studies were performed using fucosyl peroxidase coupled to colloidal gold. In ultrathin sections as well as in specimens prepared in toto for TEM an intensive binding of fucosylperoxidase-colloidal gold was predominantly found at the apical part of the acrosome appearing as a crescent-like area. In some cases this binding pattern was replaced by a triangle-like intensive labelling at the equatorial segment as revealed clearly by specimens prepared in toto. By SDS-PAGE of the SDS-extractable sperm-proteins, followed by transblotting to nitrocellulose and visualization with the fucosylperoxidase by enzymatic amplification with 4-chloro-1-naphthol mainly one protein with the reduced molecular weight of approximately 53 kdal and some small proteins with apparent molecular weights less than 20 kdal was found to be responsible for the fucose-binding ability of porcine spermatozoa.
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PMID:Evidence for a fucose-binding protein in boar spermatozoa. 390 13

Endothelia lining the 2 surfaces (arterial and ventricular) of the posterior cusp of aortic valves from normocholesterolemic, New Zealand white rabbits were found to display pleomorphic surface features characterized by differences in cellular shape and orientation to the direction of blood flow, microappendage populations (microvilli and blebs), nuclear contours and the surface reactions of cationic dyes (RR, AB) and peroxidase-conjugated lectins (Con A, Limulin, WGA). With the aid of SEM and TEM, the cells lining the arterial surfaces appeared relatively smooth and flattened with a moderate to heavy reaction of the carbohydrate cell coat at the blood interface. By contrast, the contours of the endothelia lining the ventricular surfaces were noticeably raised with numerous plasmalemmal microappendages and only a moderate dye/lectin reaction. Observations of similar endothelial populations from diet-induced, hypercholesterolemic rabbits (500 mg/dl) revealed a variety of dramatic changes in the cells lining the arterial surfaces of the valvular cusps. No severe changes were observed in the endothelia of the ventricular surfaces. Such findings are suggestive further of the importance of the interaction between the environment and the endothelial cell coat as influencing factors in the onset of intramural lipid infiltration.
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PMID:Surface responses of aortic valve endothelia from diet-induced, hypercholesterolemic rabbits. 399 84

Single-cell suspensions from the granulomas of leprosy cases were prepared for an in vitro study of the properties of the infiltrating cells. Biopsies from 44 untreated patients with tuberculoid and lepromatous leprosy were analyzed. The granulomas were found to contain lymphocytes and "large cells" (epithelioid cells and macrophages). The number of lymphocytes was significantly higher in the suspensions from the tuberculoid granulomas in comparison to the suspensions from the lepromatous granulomas. A high percentage of lymphocytes from the tuberculoid granulomas formed rosettes with sheep erythrocytes, and also showed the presence of esterase as dots in the cytoplasm. However, the lymphocytes did not form rosettes with EAC. Most of the "large cells" from both types of granulomas were esterase positive, exhibited peroxidase activity, and did not carry receptors for C3. A high percentage of "large cells" in the tuberculoid granulomas was nonadherent to a plastic surface, while the lepromatous granulomas contained a high proportion of adherent "large cells."
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PMID:In vitro studies on dermal granulomas of human leprosy--cellular characteristics. 399 63

The development of protein bodies in proteinoplasts of tobacco (Nicotiana tabacum L. var. Wis. 38) roots was investigated with TEM, HVEM, and enzyme cytochemistry. These plastids contain a three-dimensional network of fenestrated tubules which originate from invaginations of the inner membrane of the plastid envelope. Elaboration of the network occurs in parallel with cell differentiation: slender tubules common to plastids in meristematic cells undergo dilation as protein accumulates during cell differentiation; proteinoplasts of vacuolate and root cap cells usually contain a large protein body. The contents of the peripheral tubules, originating from the inner membrane, are less electron dense than the tubules making up the central network. Localized dilations within the tubular network result in the formation of dense spheroidal structures, protein bodies, apparently as a result of continued protein accumulation via tubules connecting to the central network. Protein might be imported from segments of rough ER attached to or apposed to the outer membrane of the proteinoplast envelope. The presence of catalase (E.C. 1.11.1.6), peroxidase (E.C. 1.11.1.7), and cytochrome oxidase (E.C. 1.9.3.1) was demonstrated by cytochemistry with diaminobenzidine (DAB) as substrate. Oxidized DAB was found in protein bodies after incubation in each of the specific reaction media. While aminotriazole and sodium azide inhibited oxidation of DAB by catalase and peroxidase, respectively, only potassium cyanide completely inhibited oxidation of DAB in protein bodies. We conclude that protein bodies of proteinoplasts in tobacco roots are not sites for storage of protein, rather protein bodies contain heme protein(s) with strong oxidase activity that may convey a specific function to proteinoplasts.
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PMID:Development and enzyme activity of protein bodies in proteinoplasts of tobacco root cells. 404 99

Membrane complement receptors have been identified on a subpopulation of normal lymphocytes containing cytoplasmic inclusions called parallel tubular arrays (PTA) using two different rosetting techniques. The first technique utilizes as indicator cells erythrocytes that were coated with complement by the classic pathway of complement activation (EAC rosettes). The second technique utilizes as indicator cells Salmonella typhi, which were coated with complement by the alternate pathway of complement activation (FBC rosettes). In the latter technique, lipopolysaccharide material in the bacterial cell wall directly activates complement without the use of a sensitizing antibody. This eliminates binding of marker particles by lymphocytes having Fc receptors. The presence of PTA lymphocytes at the center of EAC rosettes and FBC rosettes was demonstrated by electron microscopy, indicating that the PTA lymphocyte has a complement receptor. Examination of FBC rosettes revealed that the adherent complement-coated bacteria were usually partially surrounded by pseudopodal extensions of the PTA lymphocyte. In addition, some PTA lymphocytes phagocytized the complement-coated bacteria but not the complement-inactivated bacteria. These phagocytic cells were placed in the lymphocytic series instead of the monocytic series by virtue of complete lack of endogenous peroxidase activity.
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PMID:Complement receptors on normal human lymphocytes containing parallel tubular arrays. 624 18

The pericytes of capillaries are interesting cells which resemble the smooth muscle cells of larger vessels in some aspects of their morphology and behavior. In this report, their relationship to the underlying endothelium has been investigated in some detail. Using indirect, fluorescent immunocytochemical techniques on fresh and fixed tissues, it was found that fibronectin (an adhesive protein in many tissue culture systems) is concentrated in spots along vessels and is only faintly visible in the basement membranes of exhaustively perfused preparations. By electron microscopy, using a peroxidase immunocytochemical marker, these concentrations of fibronectin were seen to be localized to the pericyte-endothelial interstitia. Examination by TEM using a new fixation procedure demonstrated the organization of microfilaments and dense plaques along the pericyte membrane with fibrous and basement membrane-like material within this interstitial space. The arrangements of these elements suggest a mechanical linkage between the two cells. Such a linkage would allow contractions or relaxation of the pericyte to affect vessel diameter.
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PMID:Fibronectin in the microvasculature: localization in the pericyte-endothelial interstitium. 634 2

The occurrence and distribution of renin was investigated in meso- and metanephric kidneys of pig embryos in various gestational stages. The immunohistochemical peroxidase-antiperoxidase-method (PAP) was used on paraffin sections after application of an antiserum against mouse renin which cross reacts with pig renin. Renin immunoreactivity was already found in the mesonephros of 21 day pig embryos (crown-rump(CR)-length 12 mm) with the strongest reaction in the media of the juxtaglomerular afferent arteriole. Efferent vessels, mesonephric arteries, and the aortic wall also contained scattered renin-positive cells. In the definitive kidney, renin was not detected prior to the 25 mm CR-length-stage. In 45 mm embryos, immunocytochemical staining was observed not only in the media of kidney arteries and arterioles, but also in proximal tubules after pinocytic absorption of filtered renin. TEM-studies revealed that the media of both the mesonephric and the developing metanephric arteries and arterioles contains epithelioid cells whose ultrastructure is very similar to that of renin-producing cells in the adult organ. The observed distribution of renin-producing cells along the entire renal arterial tree points to the possibility that the major function of the renin-angiotensin system in the fetal animal is to participate in the stabilization of renal perfusion pressure.
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PMID:Renin immunohistochemistry in the mesonephros and metanephros of the pig embryo. 639 18

Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes: the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also visualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.
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PMID:Microtubules and microfilaments in ageing hamster embryo fibroblasts in vitro. 668 52

We used concanavalin A (con A)-peroxidase-iron dextran-diaminobenzidine (DAB) technique for the electron microscopic detection of con A binding sites on cell membranes. Normal bladder mucosa showed a sparse distribution of con A binding sites with both transmission (TEM) and scanning (SEM) electron microscopy, but bladder tumors showed a higher concentration in the distribution of con A binding sites in proportion to the histopathological grade of transitional cell carcinoma. Quantitative estimation of the con A binding sites was attempted using scanning X-ray pulse analysis of iron elements contained in the reaction complexes. Con A binding sites were quantitatively the smallest in normal mucosa, increasing proportionate to the grade of the bladder tumor. Some specimens were compared by the ferritin-labelled method and the pattern of ferritin conjugates distribution was similar to that seen with the con A-peroxidase-iron dextran method.
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PMID:Distribution of concanavalin A binding sites on normal human urinary bladder mucosa and bladder tumors by transmission and scanning electron microscopy and X-ray microanalysis. 674 Aug 37


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