Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1275122 (TEM)
 21,810 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

After intraperitoneal implantation into Swiss Silver rabbits, V2 rabbit carcinoma cells invade the mesentery where they form nodules of different size and texture: compact (less than 120 microns in diameter), loose (120-250 microns) and mixed (above 200 microns). Together with tumor development, certain changes take place in the loose connective tissue of the mesentery. Application of TEM, together with use of safranin O, has shown that, in areas free of tumor growth, collagen bundles become thick and heavy and proteoglycan density is increased. Concurrently, the number of fibrocytes, now transformed to fibroblasts, increases. Small, compact nodules are surrounded by a concentrically arranged extracellular matrix. Its overall density is similar to that of nodule-free areas. In the immediate vicinity of large, loose nodules, all constituents of the extracellular matrix disappear. Adjacent connective tissue is partly destroyed but still contains collagen fibers and proteoglycans. These findings suggest the following: The presence of V2 carcinoma cells induces marked alterations in the structured and non-structured components of the extracellular matrix. These changes are, at the same time, progressive and regressive and the occurrence of one or the other depends on local tumor progression. Progressive alterations may result from an increased activity of fibroblasts. Since degradative effects, on the other hand, are only seen in the immediate vicinity of larger tumor aggregates, it is assumed that a minimal number of tumor cells is essential for destruction of extracellular matrix.
Int J Cancer 1984 Oct 15
PMID:Morphology of peritumoral proteoglycan alterations in the rabbit mesentery invaded by V2 carcinoma cells. 649 Feb 6

Lymphocytes of 36 patients with malignant Non-Hodgkin lymphomas (NHL) were characterized by electrophoretic mobility (EPM) and EAC-rosette and E-rosette formation. Unimodal cytopherograms found in patients with low-grade malignant NHL are compatible with a monoclonal origin of the proliferating lymphoid cells. Within the CLL subgroup, deviations from the general mean EPM value in the intermediumrange were not unequivocally related to the B- or T-cell origin of the lymphocytes and remain to be explained. Comparing distinct entities of low-grade malignant NHL characterized by lymphocyte arrest at a certain stage of differentiation (Kiel Classification) we found an increase of the mean EPM in the direction of CLL----lymphoplasmacytoid immunocytoma----polymorphic immunocytoma. This arrest of leukemic cells at a certain EPM level may support a cytogenetically oriented subclassification of NHL. The Limitations of the diagnostic value of cytopherograms and rosette formation in malignant NHL--especially in those of high malignancy--are outlined.
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PMID:Cell electrophoretic characterization of lymphoid cells from various sources in patients with malignant non-Hodgkin lymphomas. 652 83

7-Oxabicyclo(2.2.1)-5-heptene-2,3-dicarboxylic anhydride has been found to possess antitumour activity against Ehrlich ascites carcinoma cells. The tumour cells incubated with the drug showed a decrease in the viable counts and cell proliferation. These effects were confirmed by in vivo studies in Swiss albino mice. The compound has a direct cytotoxic effect on the tumour cells. Vacuolization and disruption of the cytoplasm accompanied by unequal nuclear division and scattered chromosomes were recorded. In addition, 250 and 10 mg/kg were found to be the MTD and MED respectively. A dose of 25 mg/kg injected i.p. for 5 consecutive days in the tumour-transplanted animals caused a significant increase in their survival period. The compound has been shown to have a significant inhibitory effect on the DNA and RNA biosynthesis of EAC cells after 3 hr of administration; the protein biosynthesis was less affected. Meanwhile, the cellular contents of these metabolites were significantly reduced.
Eur J Cancer Clin Oncol 1982 Aug
PMID:A new antitumour substance, 7-oxabicyclo (2.2.1)-5-heptene-2,3-dicarboxylic anhydride. 689 27

Lymphocytes from 22 patients with chronic myeloid leukemia (CML), 13 treated with polychemotherapy, eight by monochemotherapy, and one untreated, were analyzed for the presence of classic T and B cell surface markers (E-rosette, EAC-rosette, surface immunoglobulins) and for their ability to respond to phytohemagglutinin (PHA). The absolute number and percentage of E-rosetting cells (T-cells), EAC-rosetting cells and cells staining for surface immunoglobulins (B cells) were all significantly lower than controls (P less than or equal to 0.025). The response to PHA was also significantly lower in patients than in controls at the smaller concentrations of the mitogen (3.75 micrograms/ml, 30 micrograms/ml) tested (P less than or equal to 0.01); at a higher PHA concentration (120 micrograms/ml) the decrease in PHA stimulation approached significance (P = 0.07). These lymphocyte abnormalities support the concept that CML lymphocytes may be derived from the leukemic clone.
Cancer 1982 Feb 15
PMID:Cell membrane markers and phytohemagglutinin reactivity of circulating lymphocytes from chronic myelocytic leukemia patients. 694 3

The surface properties of blood lymphocytes from treated myeloma patients and healthy controls were studied in vitro. The patients were tested 6 weeks after the last treatment to allow time for cells to recovery from possible drug toxicity. Peripheral-blood lymphocytes were tested for rosette formation with unsensitized sheep erythrocytes (E rosettes) and with complement and antibody-coated erythrocytes (EAC rosettes). The tests were duplicated using lymphocytes pretreated with trypsin. As others have noted, myelomatosis is associated with increased blood levels of EAC-rosette-forming cells and a marked reduction in E-rosette-forming cells. E-rosette formation was significantly increased by pretreatment of myeloma lymphocytes with trypsin. By contrast, enzyme-treated cells showed no significant change in EAC-rosette formation. These results suggest that the absolute number of circulating T cells is probably not reduced in myelomatosis, but that the surface of T cells is somehow modified so that a proportion of them lose the ability to form E rosettes.
Br J Cancer 1980 Feb
PMID:Enzyme-induced modification of the surface properties of lymphoid cells in malignant disease. I. Effect of trypsin on rosette formation by lymphocytes in myelomatosis. 696 55

In 166 patients with lung carcinoma peripheral blood lymphocytes and their subpopulastions (AE-RFC, TE-RFC, EAC-RFC) were examined. In comparison with control--the mean values of the absolute counts of the blood lymphocytes did not show statistically significant changes in various stages of lung cancer. However, the count of active T lymphocytes as well as total T lymphocytes were slightly lowered in the stage I of the disease, and significantly decreased in stages II and III. The lowest decrease of T lymphocytes was found in adenocarcinoma and the greatest--in microcellular cancer.
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PMID:Peripheral blood lymphocytes and their subpopulations in lung carcinoma. 697 27

Several markers of humoral and cellular immunity were studied in 49 untreated patients with malignancies of different anatomical locations, histological types and clinical stages. The proportion of EAC rosettes, levels of immunoglobulins as well as levels of the C3 and C4 components of the complement system were within the normal range (p.n.s.). The proportion of T lymphocytes, measured by the classical E rosette method at 4 degrees C, was significantly decreased in the whole group when compared with a control population (mean 46,27 +/- 12,34, p less than 0.0005), regardless of the anatomical origin of the tumor. In patients with lung cancer (45,52 +/- 10,37), those with the epidermoid type (51 +/- 9,45) had a reduced number of E rosettes when compared with controls (58,87 +/- 5,53 p less than 0.005) but they were still greater in number than in the other histological types (43,16 +/ 9,97 p less than 0.025). We found no relationship between the proportion of E rosettes and the clinical stage. The number of T lymphocytes with E rosettes after incubation at 30 degrees C was within normal limits in most patients, except in those cases with lung cancer at an early clinical stage (23,94 +/- 5,85 p less than 0.005). Our findings would support the hypothesis of a deficiency in cellular immunity in patients with malignancies, which could favour the progression of the disease.
Bull Cancer 1981
PMID:[Lymphocytes subpopulation in cancer's patients. Relationship with primitive anatomical location, histological type and clinical stage (author's transl)]. 697 68

The measurement of enzymatic activity in the blast cell is a recent investigational technique which seems to complement immunologic measurement of cell membrane receptors in childhood lymphoproliferative malignancies. We have identified a patient with non-Hodgkin's lymphoma, not readily classifiable on the basis of either morphology or cell surface markers, i.e., E rosette and SIg negative, EAC positive. Analysis of enzymatic function, however, revealed markedly elevated ADA activity and essentially normal TdT activity. ADA positive lymphoma can arise from a primitive T-cell line. It is, thus, apparent that the morphological description of malignant lymphoid lines must be accompanied by surface marker and enzymatic data in order to properly classify cell lineage and identify appropriate therapeutic modalities.
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PMID:Adenosine deaminase positive, E receptor negative lymphoma. 698 56

Cell populations in afferent lymph of human leg were defined by surface characteristics and cytotoxic activity in 7 normal men and 9 patients with localized cancer. A higher percentage of E-rosette forming cells was found in lymph (78.5 and 83.0) than in blood (60.0 and 63.0 p less than 0.05). The percentages of lymph EA-RFC were 10.3 and 18.0, of EAC-RFC 13.1 and 8.0, of surface immunoglobulin carrying cells 3.0 and 3.1. In blood 20.6 and 18.0 percent of cells formed EA-rosettes, 23.0 and 15.6 EAC-rosettes, 5 and 9.5 contained surface immunoglobulins. The differences between lymph and blood EA- and EAC-RFC in normals were statistically significant (p less than 0.05). In cancer patients only lymph-blood differences for S Ig+ were significant (p less than 0.05). No significant differences were found between normals and cancer patients. In both groups, the natural cytotoxicity against K 562 cells was 6 times lower in lymph as compared to blood (p less than 0.05), the cytotoxicity in those with cancer was higher than in normals (p less than 0.05). The study indicates that B cells have a limited tendency toward leaving the blood circulation and migrating through tissues. Moreover natural killer cells do not seem to belong to the recirculating pool of lymphocytes.
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PMID:Immunologic characteristics of human peripheral lymph cell populations. 720 86

Incubation of peripheral blood lymphocytes from patients with bladder cancer in bestatin (specific inhibitor of aminopeptidase B and leucine aminopeptidase) solution resulted in a significant increase in the proportion of E-rosette-forming lymphocytes. The increase in the proportion of E-rosette-forming lymphocytes was abolished after reincubation with 100% autochthonous serum. The fraction of E-rosette-forming lymphocytes from patients with bladder cancer which had restored receptors for sheep erythrocytes (SRBC) by incubation with bestatin also expressed receptors for the Fc portion of IgG and IgM. The proportion of EAC-forming lymphocytes remained unchanged after the incubation with bestatin. When 600 mg of bestatin was administered for 1 day to patients with bladder cancer, restorative effect on the proportion of E-rosette forming lymphocytes was also demonstrated. The proportion of EAC-forming lymphocytes, however, did not change significantly.
J Cancer Res Clin Oncol 1980
PMID:Restoration of E-rosette formation by bestatin in patients with bladder cancer. 721 83


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