Gene/Protein
Disease
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Drug
Enzyme
Compound
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Target Concepts:
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Query: UMLS:C0920646 (
renal ischemia
)
2,515
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Oxidative stress has been considered as the possible mechanism of
renal ischemia
/reperfusion injury.
L-carnitine
is an endogenous mitochondrial membrane compound and could effectively protect ischemia-reperfusion injury in the kidney. To elucidate the nephroprotective effects of
L-carnitine
, here we assessed the effect of
L-carnitine
on hydrogen peroxide (H(2)O(2))-mediated oxidative stress in the human proximal tubule epithelial cell line, HK-2 cells. The results showed that pretreatment with
L-carnitine
12h inhibited H(2)O(2)-induced cell viability loss, intracellular reactive oxygen species generation and lipid peroxidation in a concentration-dependent manner. Also
L-carnitine
promoted endogenous antioxidant defense components including total antioxidative capacity, glutathione peroxidase, catalase and superoxide dismutase. In parallel, cell apoptosis triggered by H(2)O(2) characterized with the DNA fragment and caspase-3 activity were also inhibited by
L-carnitine
. Furthermore, mitochondrial dysfunction associated with cell apoptosis including membrane potential loss, down-regulation of Bcl-2 and up-regulation of Bax and the release of cytochrome c were abrogated in the presence of
L-carnitine
. These results suggested that
L-carnitine
could protect HK-2 cells from H(2)O(2)-induced injury through the inhibition of oxidative damage, mitochondria dysfunction and ultimately inhibition of cell apoptosis, which indicates that
L-carnitine
may be a promising approach for the treatment of oxidative stress in renal diseases.
...
PMID:L-carnitine attenuates oxidant injury in HK-2 cells via ROS-mitochondria pathway. 2009 44
