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Query: UMLS:C0851341 (infestation)
 10,121 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Ergosterol was used as a specific indicator of fungal biomass to determine and assess mould growth on damp building material. The samples were saponified, cleaned up on a silica gel column and the sterols silylated and analysed by gas chromatography and mass spectrometry in MS/MS mode. Ninety-one samples of building materials from damp rooms were analysed including plaster, plaster/paint, paint, wood-chip wallpaper and paper wall covering. The concentrations of ergosterol ranged from 0.1 to 130 microg/g dry mass and depended on carbon content of the material. The highest concentrations were determined for wallpaper, the lowest for plaster and intermediate ones for paint. Based on ergosterol content and inspection of the room during sampling a rough classification of mould infestation is presented. The applicability of the ergosterol method was further tested in two case studies on the spatial distribution of fungi on damp walls and irregular distributions were found. With few exceptions the concentration of ergosterol in building materials was found to be a suitable indicator to estimate fungal biomass.
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PMID:Ergosterol as an indicator of mould growth on building materials. 1547 Nov 2

Ergosterol is considered to be a suitable indicator of mold infestation in barley and malt. In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high-performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program), and applied to 124 samples of barley and malt. Ergosterol was isolated by extraction and saponification, and the quantification was performed using HPLC with diode array detection. The content of ergosterol ranged between the limit of detection (LOD) and 36.3 mg/kg in barley and between the LOD and 131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens, and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.
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PMID:Determination of ergosterol levels in barley and malt varieties in the Czech Republic via HPLC. 1847 97