Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0851184 (thinning)
 11,252 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Chemical destruction of the olfactory mucosa leads to a neuronal regeneration. A new organotypic culture model is perfected to improve the regenerating processes study. Explants of neuroepithelium attached to olfactory bulbs were removed from adult mice and cultured, 12 h after ZnSO4 intranasal application. After 3 days in culture, explants showed a necrosis in the olfactory epithelium and a thinning of the olfactory bulb nervous layer. From the fifth day of culture, and mostly the tenth, new cells showed positive immunoreactivity with the olfactory marker protein (OMP), meaning they were regenerating olfactory neurons. Simultaneously, OMP immunoreactivity increased in the nervous and glomerular layers of the olfactory bulb, indicating epithelio-bulbar reconnection. This organotypic culture model could allow further investigations on the regenerating process kinetic.
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PMID:Organotypic culture of neuroepithelium attached to olfactory bulb from adult mouse as a tool to study neuronal regeneration after ZnSO4 neuroepithelial trauma. 1050 2

Previous studies showed that uptake of the lectin conjugate, wheat germ agglutinin-horseradish peroxidase (WGA-HRP) by olfactory receptor cells results in a thinning of the olfactory epithelium (OE) and increased turnover of globose basal cells. To ascertain the cell-type lost as well as the time course and mechanism of the loss, the current study measured changes in the number of dendritic knobs, olfactory marker protein (OMP) expression and assessed TUNEL labeling as an indicator of apoptosis. Electron microscopic analysis of the number of dendritic knobs showed that the largest reduction occurred at 1 week after intranasal irrigation with WGA-HRP. This data in conjunction with decreased OMP staining provided evidence for a loss of mature receptor neurons. TUNEL labeling, especially in more superficial aspects of the OE, peaked at 18 hr after WGA-HRP application suggesting that the lectin-conjugate produced a rapid induction of apoptotic cell death that was complete by 3 days. Measurement of tyrosine hydroxylase (TH) activity in the olfactory bulb, a sensitive measure of deafferentation, showed that innervation reached a nadir at about 1 week and that reinnervation was complete by 4 weeks. These findings demonstrate that internalization of WGA-HRP by some receptor cells results in their death by apoptosis and a subsequent deafferentation of the olfactory bulb.
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PMID:Lectin-induced apoptosis of mature olfactory receptor cells. 1199 66