Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0851184 (
thinning
)
11,252
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The emergence of 3D bioprinting is expected to solve the present puzzle in the field of regenerative medicine. However, the appropriate bioink was lacking due to the rigorous requirement of high printability and biocompatibility, which was often contradictory. In this study, a novel thixotropic magnesium phosphate-based gel (TMP-BG) was prepared and its application in 3D printing was explored. The stable gel could be synthesized by adjusting the ratio of ternary reactants (NaOH, Mg(OH)
2
, and H
3
PO
4
). Moreover, the structure, morphology, particle size and composition of
TMP
-BG were characterized. Furthermore, the rheological and thixotropic behaviors and degradation of
TMP
-BG were investigated. The printability of
TMP
-BG was tested by using the extrusion-based 3D printer. The biocompatibility of
TMP
-BG was evaluated in vitro. The composition of
TMP
-BG was MgNa
3
H(PO
4
)
2
, which was of nanometer and sub-micro scale and easily formed a complex three-dimensional porous structure. Rheological results showed that the gel had notable shear
thinning
behavior and good thixotropy, which could provide the
TMP
-BG with injectability and formability simultaneously. In addition, the thixotropic mechanisms of
TMP
-BG were speculated to be a model of "house of cards". Finally,
TMP
-BG could be printed into large-sized and different complex three-dimensional structures. Results of the MG-63 cell viability and cell proliferation confirmed the biocompatibility of TMG-BG. The present newly developed
TMP
-BG has the potential to be used as 3D printing bioink involving living cells for future applications in regenerative medicine.
...
PMID:A novel thixotropic magnesium phosphate-based bioink with excellent printability for application in 3D printing. 3225 67
3D Bioprinting is expected to become a strong tool for regenerative medicine, but satisfactory bioinks for the printing of constructs containing living cells are lacking due to the rigorous requirement of high printability and biocompatibility, which are often contradictory. Here, we have reported the development of a novel hybrid bioink by combining rigid gellan gum (GG), flexible sodium alginate (SA), and a bioactive substance thixotropic magnesium phosphate-based gel (TMP-BG). The ratio of these components was first optimized to obtain satisfactory gelating, mechanical, rheological, and printing properties. The formulated hybrid GG-SA/
TMP
-BG bioink had a good printability due to the shear-
thinning
and its multiple cross-linking by Mg
2+
and Ca
2+
. The tunable mechanical performance of the hybrid bioink could simulate various extracellular matrices of the different tissues and support integrity of 3D printing constructs. Moreover, the hybrid bioink induced apatite deposition during immersion in simulated body fluids, and also promoted cell proliferation in vitro. MG-63 osteosarcoma cells were dispersed in the bioink and printed into 3D constructs. The cells exhibited good cell survival due to the shear-
thinning
property of the bioink and the ion concentration used for cross-linking. The proliferation rate of the cells also significantly exceeded those in non-printed samples. Confocal microscopy revealed a homogeneous distribution of cells in the printed constructs, and survival for more than 7 d. In vivo animal experiments showed that the hybrid bioink without cells could induce osteochondral repair. Therefore, this hybrid bioink has good printability, biocompatibility, mechanical support, and bioactivity, which is expected to have promising applications in 3D bioprinting.
...
PMID:3D Bioprinting of shear-thinning hybrid bioinks with excellent bioactivity derived from gellan/alginate and thixotropic magnesium phosphate-based gels. 3248 94
