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Query: UMLS:C0849640 (skin damage)
 1,516 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Few studies have indicated the use of antioxidants in the protection against cancers. The goal of this study was to develop a tissue culture model using MRC-5 cells, which would allow for rapid testing of treatments for skin damage, mediated by exposure to ultraviolet radiation (UVR). Overall the specific aims were to evaluate the morphological and pathophysiological responses of MRC-5 fibroblast cells exposed to UVR and antioxidant supplementation. In the first phase, MRC-5 cells were exposed to UVR for 30, 45 or 60 minute time intervals, and cellular damage was assessed biochemically and morphologically. In the second phase, cells were treated for 45 minutes in the presence or absences of physiological levels of vitamin E. The cells were evaluated after 24, 48, and 72 hours for biochemical and morphological changes. Structural changes were noted throughout the experimental phase among the UVR exposed groups compared with native control. However, treatment with vitamin E pre and post exposure provided a protective role by demonstrating lower levels of MDA (p < 0.05) as well as normal structural appearance.
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PMID:The effects of vitamin E on the proliferation and viability of irradiated fibroblast-like cells. 1208 62

The goal of this study was to develop a tissue culture model with and without cellular protection (anti-oxidants) in an attempt to prevent or treat squamous damage as a result of free radical generation. The specific aim was to evaluate the morphological responses of MRC-5 fibroblast cells exposed to UV-radiation and vitamin E supplementation. The criteria for the morphological evaluation was as follows; cell and nucleus shapes, size, N/C ratio, nucleus pleomorphism, hydropic swelling, as well as other features or characteristics of membrane and cytoplasmic alterations. The experimental design was divided into two phases. In the first phase the cells were exposed to radiation for 30, 45 and 60-minute intervals, the morphological evaluation was assessed using Image Pro Digital Analysis technique. In the second phase, vitamin E was administered to the cells before and after exposure to 45 min of radiation. Data obtained demonstrated that, forty-five minutes of radiation exposure caused traumatic stress to the cells, swelling, cellular debris and fragmentation. However, treatment with vitamin E pre and post exposure provided protection for the cells. Vitamin E treatment resulted in less cellular aggregation, more abundant eosinophilic cytoplasm and an overall healthy appearance. The data suggest that the use of vitamin E may minimize skin damage by protecting the integrity of the fibroblast cells for up to 72 hours in culture.
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PMID:Morphometric analysis of MRC-5 fibroblast like cells exposed to intermittent UV radiation. 1272 29