Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0848332 (
Spots
)
453
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Epigallocatechin gallate
(
EGCG
) is the major active polyphenol in green tea. Protein interaction with
EGCG
is a critical step in the effects of
EGCG
on the regulation of various key proteins involved in signal transduction. We have identified a novel molecular target of
EGCG
using affinity chromatography, two-dimensional electrophoresis, and mass spectrometry for protein identification.
Spots
of interest were identified as the intermediate filament, vimentin. The identification was confirmed by Western blot analysis using an anti-vimentin antibody. Experiments using a pull-down assay with [3H]
EGCG
demonstrate binding of
EGCG
to vimentin with a Kd of 3.3 nm.
EGCG
inhibited phosphorylation of vimentin at serines 50 and 55 and phosphorylation of vimentin by cyclin-dependent kinase 2 and cAMP-dependent protein kinase.
EGCG
specifically inhibits cell proliferation by binding to vimentin. Because vimentin is important for maintaining cellular functions and is essential in maintaining the structure and mechanical integration of the cellular space, the inhibitory effect of
EGCG
on vimentin may further explain its anti-tumor-promoting effect.
...
PMID:The intermediate filament protein vimentin is a new target for epigallocatechin gallate. 1571 70
