Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0848237 (acute stress)
 4,619 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An automated spectrophotometric method for total esterase activity (TEA) measurement in porcine saliva has been developed and validated, using 4-nitrophenyl acetate (4-NA) as substrate. The method was precise and accurate, with low limit of detection, and was able to measure samples with TEA activities up to 400IU/L without any dilution. In addition, the different enzymes contributing to TEA were characterized, being identified carbonic anhydrase VI (CA-VI), lipase, cholinesterase (ChE) and cholesterol esterase (CEL). TEA significantly increased (1.49-fold, P<0.01) in healthy pigs just after applying an acute stress stimulus consisting of nasal restraint, being lipase and cholinesterase the main responsible of this increase. TEA was significantly increased (1.83-fold, P<0.001) in a group of pigs with lameness; in this case, in addition to lipase and ChE, CA-VI also increased. The results found in this report indicate that TEA can be easily measured in porcine saliva with an accurate and highly reproducible automated method. Salivary TEA is mainly due to the activity of four enzymes: CA-VI, lipase, ChE and CEL, and these enzymes can change in a different way in situations of stress or disease.
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PMID:Total esterase measurement in saliva of pigs: Validation of an automated assay, characterization and changes in stress and disease conditions. 2844 10

Acute stress induces an array of behavioural reactions in horses that vary between individuals. Attempts to relate behavioural patterns and physiological responses have not always given clear-cut results. Here, we measured the changes in a panel of salivary components: salivary alpha-amylase (sAA), lipase, total esterase (TEA), butyrylcholinesterase (BChE), adenosine deaminase (ADA), and cortisol, and their potential link with horses' behaviours after acute stress. Saliva samples were collected in nine riding horses subjected to a test consisting of opening an umbrella. Saliva sampling was obtained at a basal time point in the stall (T1), in the test indoor arena (T2), at a time of stress (T3), and 30 min (T4) and 60 min (T5) later. The horses' behaviour was recorded at T3 for 1 minute. sAA, lipase, TEA, and BChE showed significant changes along time, increasing at T3 for BChE, and decreasing at T4 for sAA and BChE. Butyrylcholinesterase appeared to be the most reliable predictor of behavioural responses, as it correlated with the index of emotionality, of laterality, and the occurrence of alarm signals, while sAA decreased when horses expressed quieter behaviours. These first results bring promising lines for novel, more precise physiological markers of acute stress in horses that can bridge the gap between behaviour and physiology.
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PMID:Changes in Saliva Analytes Correlate with Horses' Behavioural Reactions to An Acute Stressor: A Pilot Study. 3175 94