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Query: UMLS:C0848237 (acute stress)
 4,619 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In young chicks submitted to acute stress by forced swimming there was a significant increase in the number of the measurable [3H]-flunitrazepam receptors in synaptosomal membranes from forebrain. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]-flunitrazepam receptor number in synaptosomal membranes from non-stressed chicks. However, this Triton X-100 stimulatory effect was not observed when tested in synaptosomal membranes from stressed chicks. In all cases the affinity remained unchanged. This result suggest that: (i) acute stress and Triton X-100 induce receptor recruitment by enhancing [3H]-flunitrazepam accessibility to a pool of receptors which is unmeasurable either before stress or in absence of detergent; (ii) neither recruitment types are additive and they involve receptors coming from the same nonmeasurable pool; (iii) stress induces a maximal recruitment of existing benzodiazepine receptors; (iiii) the pool of nonmeasurable receptors represents about a quarter of the total in control chicks. The recruitment at a short time of stress could be interpreted in terms involving internalization; recycling or modulation of receptors but not its biosynthesis or degradation.
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PMID:Benzodiazepine receptor recruitment after acute stress in synaptosomal membranes from forebrain of young chicks: action of Triton X-100. 131 85

A significant increase in the number of measurable [3H]Ro 5-4864 receptors was found in forebrain membranes of chicks submitted to 15 min of acute swim stress compared to non-stressed chicks. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]Ro 5-4864 receptor number in forebrain membranes from non-stressed chicks. However, this increase caused by Triton X-100 was not observed when tested in forebrain membranes from stressed chicks. In all cases the affinity remained unchanged. These results suggest that: (1) acute stress and Triton X-100 induce receptor increase by enhancing [3H]Ro 5-4864 accessibility to a pool of receptors not detected before stress or in the absence of detergent; (2) the pool of non-measured receptors represents about a third of the total in control chicks; (3) the increments are not additive and could involve receptors coming from the same non-measured pool; (4) the receptor increase during a short time of stress could be explained by recruitment of receptors but not by an increase in the receptor protein biosynthesis; (5) stress induces a maximal recruitment of measurable [-3H]Ro 5-4864 receptors.
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PMID:Recruitment of peripheral-type benzodiazepine receptors after acute stress in chick forebrain membranes: action of Triton X-100. 874 Apr 50

The [3H]-flunitrazepam receptor density, measured ex vivo in synaptosomes at 4 degrees C, increased by about 30% because of acute stress in chicks. This increase was first reported to be a receptor recruitment due to the fact that the increase induced by subsolubilizing concentrations of Triton X-100 was not additive to the receptor increase induced by acute stress [J Neural Transm 87 (1992) 97]. In synaptosomal membranes from stressed chicks, the incorporation of alkaline phosphatase or ATP into the lumen abolished or increased, respectively, the receptor unmasking after incubation at 4 and 37 degrees C, suggesting that phosphorylation plays a role in the recruitment mechanism. Moreover, both colchicine and vinblastine, but not taxol, abolished the recruitment induced by stress at 37 degrees C only in synaptosomes, suggesting that micrutubule depolymerization plays a role in the masking of receptors. Furthermore, both cytochalasins C and D induced an increase of the receptor density, abolished by N-ethylmaleimide, in both the stressed and nonstressed conditions, suggesting that microfilament depolymerization induced the exposure to the radioligand of a cytosolic vesicular receptor pool, which had not fused yet with the postsynaptic membrane.
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PMID:Effects of phosphorylation and cytoskeleton-affecting reagents on GABA(A) receptor recruitment into synaptosomes following acute stress. 1217 45