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Target Concepts:
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Query: UMLS:C0848237 (
acute stress
)
4,619
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Although many studies have investigated the function of cellular prion protein (
PrPc
), its physiologic role remains elusive.
PrPc
null mice have been reported to develop normally and to show normal performance in most behavioural tests. In the present study we investigated whether this also holds true after episodes of
acute stress
.
PrPc
gene ablated (Prnp0/0) and wild-type mice were subjected to restraint stress, electric foot shock, or swimming and compared with non-stressed animals. Immediately after the stressful situation, the anxiety levels and locomotion of the animals were measured using plus-maze and open-field tests. Among non-stressed animals, there was no significant difference in performance between Prnp0/0 and wild type animals in either test. However, after
acute stress
provoked by a foot shock or a swimming trial, Prnp0/0 animals showed a significant decrease in anxiety levels when compared with control animals. Moreover, after the swimming test, knockout mice presented decreased locomotion when compared to wild-type mice. Because of this observation, we also assessed both types of mice in a forced swimming test with the objective of better evaluating muscle function and found that Prnp0/0 animals presented reduced forced swimming capacity when compared to controls. As far as we know, this is the first report suggesting that cellular prion protein is involved in modulation of anxiety or muscular activity after acute psychic or physical stress.
...
PMID:Altered behavioural response to acute stress in mice lacking cellular prion protein. 1597 Feb 15
As manifestations of prion diseases include disturbances of hypothalamic and pituitary functions, we tested the hypothesis that the cellular prion protein (PrPC) has a role as modulator of the hypothalamic-pituitary-adrenal axis. The level of corticosterone and adrenocorticotropic hormone were compared in PrPC null (
PrP
0/0) and wild-type (PrP+/+) mice.
PrP
0/0 showed hypercorticism during the dark part of day. After
acute stress
, corticosterone and adrenocorticotropic hormone increased similarly in PrP+/+ and
PrP
0/0 mice. Adrenocorticotropic hormone, however, remained elevated in PrP+/+ 0/0 mice at corticosterone levels that are inhibitory in
PrP
mice. Pretreatment with corticosterone or dexamethasone inhibited stress-induced elevation of adrenocorticotropic hormone in PrP+/+ but not in
PrP
0/0 mice. Thus, PrPC may play a role in the negative feedback regulation of axis.
...
PMID:Hypothalamic-pituitary-adrenal axis disregulation in PrPC-null mice. 1879
The cellular prion protein PrP
C
is highly expressed in neurons, but also present in non-neuronal tissues, including the testicles and spermatozoa. Most immune cells and their bone marrow precursors also express
PrP
C
. Clearly, this protein operates in highly diverse cellular contexts. Investigations into putative stress-protective roles for
PrP
C
have resulted in an array of functions, such as inhibition of apoptosis, stimulation of anti-oxidant enzymes, scavenging roles, and a role in nuclear DNA repair. We have studied stress resilience of spermatozoa and peripheral blood mononuclear cells (PBMCs) derived from non-transgenic goats that lack
PrP
C
(
PRNP
Ter/Ter
) compared with cells from normal (
PRNP
+/+
) goats. Spermatozoa were analyzed for freeze tolerance, DNA integrity, viability, motility, ATP levels, and acrosome intactness at rest and after
acute stress
, induced by Cu
2+
ions, as well as levels of reactive oxygen species (ROS) after exposure to FeSO
4
and H
2
O
2
. Surprisingly,
PrP
C
-negative spermatozoa reacted similarly to normal spermatozoa in all read-outs. Moreover,
in vitro
exposure of PBMCs to Doxorubicin, H
2
O
2
and methyl methanesulfonate (MMS), revealed no effect of
PrP
C
on cellular survival or global accumulation of DNA damage. Similar results were obtained with human neuroblastoma (SH-SY5Y) cell lines stably expressing varying levels of
PrP
C
. RNA sequencing of PBMCs (
n
= 8 of
PRNP
+/+
and
PRNP
Ter/Ter
) showed that basal level expression of genes encoding DNA repair enzymes, ROS scavenging, and antioxidant enzymes were unaffected by the absence of
PrP
C
. Data presented here questions the
in vitro
cytoprotective roles previously attributed to
PrP
C
, although not excluding such functions in other cell types or tissues during inflammatory stress.
...
PMID:Stress Resilience of Spermatozoa and Blood Mononuclear Cells without Prion Protein. 2941 49
