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Target Concepts:
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Query: UMLS:C0847097 (
acidity
)
15,165
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Previous studies reported that the use of
Metschnikowia pulcherrima
in sequential culture fermentation with
Saccharomyces cerevisiae
mainly induced a reduction of volatile
acidity
in wine. The impact of the presence of this yeast on the metabolic pathway involved in pyruvate dehydrogenase (PDH) bypass and glycerol production in
S. cerevisiae
has never been investigated. In this work, we compared acetic acid and glycerol production kinetics between pure
S. cerevisiae
culture and its sequential culture with
M. pulcherrima
during alcoholic fermentation. In parallel, the expression levels of the principal genes involved in PDH bypass and glyceropyruvic fermentation in
S. cerevisiae
were investigated. A sequential culture of
M. pulcherrima
/
S. cerevisiae
at an inoculation ratio of 10:1 produced 40% less acetic acid than pure
S. cerevisiae
culture and led to the enhancement of glycerol content (12% higher). High expression levels of pyruvate decarboxylase
PDC1
and
PDC5
,
acetaldehyde dehydrogenase
ALD6
, alcohol dehydrogenase
ADH1
and glycerol-3-phosphate dehydrogenase
PDC1
genes during the first 3 days of fermentation in sequential culture conditions are highlighted. Despite the complexity of correlating gene expression levels to acetic acid formation kinetics, we demonstrate that the acetic acid production pathway is altered by sequential culture conditions. Moreover, we show for the first time that the entire acetic acid and glycerol metabolic pathway can be modulated in
S. cerevisiae
by the presence of
M. pulcherrima
at the beginning of fermentation.
...
PMID:
Metschnikowia pulcherrima
Influences the Expression of Genes Involved in PDH Bypass and Glyceropyruvic Fermentation in
Saccharomyces cerevisiae
. 2870 1
