Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0847097 (acidity)
 15,165 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Numerous factors seem to affect zinc absorption. Gastric acid secretion has been demonstrated to facilitate iron absorption. The zinc tolerance test (ZTT with ZnSO4 220 mg p.o.) was performed in 11 healthy volunteers to study the effects of administering the acid secretion inhibitor cimetidine (1 g/day p.o. for 3 days) and to evaluate the influence of HCl gastric secretion on zinc absorption in physiological conditions. Zinc absorption was reduced after cimetidine administration (p less than 0.005), suggesting that gastric pH influences zinc absorption. To rule out any direct effect of the drug on zinc absorption in five other healthy adults we further evaluated zinc absorption by using a different H2 antagonist (ranitidine 300 mg/day for 3 days and 300 mg before the test). Cimetidine was also tested in these subjects at half the dosage administered to the first group of subjects. Gastric acidity was monitored at 60-min intervals throughout the test via a nasogastric tube. The areas under the plasma concentration curves for zinc were significantly reduced after ranitidine (p less than 0.01), but not after cimetidine administration. Gastric acid was also reduced after ranitidine, but not after cimetidine (500 mg) administration, suggesting that gastric acid secretion plays a role in the regulation of zinc absorption in man.
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PMID:Inhibition of gastric acid secretion reduces zinc absorption in man. 189 92

Chemical and biological similarities of plutonium(IV) and iron(III) suggested that octadentate ligands containing hydroxamate or catecholate functional groups, which are found in microbial iron chelating agents (siderophores), would be effective and relatively selective complexing agents for actinide(IV) ions. However, their usefulness for in vivo chelation of actinide(IV) is limited, because catechol and hydroxamate are such weak acids that the potential for octadentate binding of actinide(IV) cannot be achieved at physiological pH. The structurally similar monoprotic and more acidic 1-hydroxy-2(1H)-pyridinone (1,2-HOPO) group was, therefore, incorporated into multidentate ligands. Treatment of 1,2-dihydro-1-hydroxy-2-oxopyridine-6-carboxylic acid (5) with phosgene in THF solution gives the active ester poly[1,2-dihydro-1,2-dioxopyridine-6-carboxylate], which upon treatment with excess anhydrous dimethylamine gave a 60% yield of N,N-dimethyl-1,2-dihydro-1-hydroxy-2-oxopyridine-6-carboxamide (6). A similarly reactive intermediate was prepared from 5 and an equimolar amount of phosgene in N,N-dimethylacetamide. Combined in situ with 1,3-propanediamine, benzylamine, spermine, spermidine, 1,3,5-tris(aminomethyl)benzene, or desferrioxamine B and excess triethylamine, the latter intermediate gave the corresponding amides in isolated yields ranging from 16% to 60%. The free ligands, their Zn(II) complexes, and the ferric complex of 3,4,3-LIHOPO were administered to mice [30 mumol/kg intraperitoneally 1 h after Pu(IV)-238 citrate, kill at 24 h]. Net Pu removal [Pu excretion (treated)-PU excretion (control)], expressed as percent of injected Pu, was as follows: Na salts and Zn(II) complexes, respectively, of 3-LIHOPO (54, 56), 3,4-LIHOPO (58, 60), 3,4,3-LIHOPO (73, 76); Na salts of MEHOPO (46), DFO-HOPO (78); Fe(III) complex of 3,4,3-LIHOPO (79). DFO-HOPO and 3,4,3-LIHOPO and its Zn(II) and Fe(III) complexes promoted significantly more Pu excretion than CaNa3-DTPA (61% of injected Pu). Preliminary findings on the acute toxicity of the poly(HOPO) ligands and HOPO monomers are presented in an appendix. The biological data indicate strongly that the aqueous solubility and relatively high acidity of the octadentate HOPO ligands, 3,4,3-LIHOPO and DFO-HOPO allow them to form complete eight-coordinate complexes with Pu(IV) ion.
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PMID:Specific sequestering agents for the actinides. 16. Synthesis and initial biological testing of polydentate oxohydroxypyridinecarboxylate ligands. 282 83

A review of the effect on metabolism of antacids, anticholinergics and histamine H2-antagonists is given. It is stated that long-term treatment with antacids by a variety of mechanisms can cause severe metabolic complications such as the milk-alkali syndrome and phosphorus depletion. The interaction with other drugs can also be marked. There is very little known of the effect of anticholinergics on metabolism. Clinical experience has not indicated that any serious consequences will occur. The authors have investigated the effect continuous treatment with cimetidine 400 mg at night or twice daily for three years on weight, haemoglobin, plasma iron, plasma folate, plasma vitamin B12, albumin and plasma calcium. The only significant difference that occurred was a slight decrease of plasma calcium within the reference values. In conclusion it is stated that there is very little indication that the moderate and inconstant reduction of acidity over 24 hours which can be achieved by anticholinergics or twice daily administration of presently available H2-antagonists will result in metabolic consequences arising from reduced acidity. Higher doses given more frequently might give a risk for bacterial overgrowth with metabolic consequences.
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PMID:Metabolic consequences of reduced gastric acidity. 286 50

This report summarizes a study of the chemical and biological characteristics of three oligotrophic lakes located in a region that receives a moderately acidic precipitation (mean annual pH 4.5-4.6), and a sulfate deposition of about 20 kg/ha/yr. The two brownwater lakes are relatively acidic (pH 4.5 and 4.8), and much of their acidity is attributable to organic anions. The brownwater lakes also have a large concentration of aluminum and iron, but these are bound to dissolved organic matter and are relatively non-toxic to biota. Average phytoplankton production was largest in the clearwater lake. This was due to its relatively deep euphotic zone, since the average unit-volume productivity did not differ much among the lakes. In fact, productivity at light optimum was largest in the most acidic brownwater lake, probably because of its larger phosphorus concentration. The clearwater lake had extensive macrophyte vegetation, which covered its bottom to a depth of 6.5 m. In the brownwater lakes, macrophytes were confined to shallow nearshore water because of the limited water transparency. Zooplankton density and biomass were largest in the most acidic brownwater lake, probably because of allochthonous organic particulates and little fish predation. Benthic invertebrates were abundant in all three lakes, and were dominated by insects, especially Chironomids. Lakes in the study area appear to be sustaining fish populations at more acidic pHs than elsewhere. This may be due to the large concentration of dissolved organic matter in many lakes, which complexes and partially detoxifies metals such as aluminum.
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PMID:Characteristics of three acidic lakes in Kejimkujik National Park, Nova Scotia, Canada. 292 90

Conflicting data have been reported on "sports anaemia" and anaemia during physical training. Most of these results are of studies at rest before or after training. The aim of this investigation was to further study the profiles of serum iron (Se Fe) and transferrin (Se Tr), in 14 physically trained men (28 +/- 6 years) during an exhaustive interval training session. The 45 min Square-Wave Endurance Exercise Test (SWEET) was performed on a cycle ergometer. To the SWEET base, established as a % of individual VO2max, a peak of 1 min at VO2max was added every 5 minutes. Arterial blood samples were taken at rest, during the SWEET at the 14th, 15th, 29th, 30th, 44th and 45th minutes, just before and after the peaks, and at the 15th min of recovery. Lactate, acidity [H+], PaCO2, PaO2, Haematocrit (Hct), Haemoglobin (Hb), Se Fe and Se Tr were measured. After the SWEET, weight loss was 0.89 +/- 0.15 kg. Lactate and serum iron rose progressively at the base levels and at the peaks, while PaCO2 and bicarbonate fell progressively. Hct, [Hb], serum transferrin and [H+] increased significantly at the 14th min of SWEET and thereafter no change was observed. At the 45th min with respect to the value at rest, Se Fe increased as much as +32%, Se Tr +13% and [Hb] +8%. Haemoconcentration could explain the changes in Se Tr but not the total significant increase in Se, Fe, which moreover is not explained by acidosis [H+].(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Serum iron and transferrin during an exhaustive session of interval training. 334 80

Twenty foods were cooked in iron and non-iron utensils. Also, three foods were cooked in two iron skillets. Three replications were made, and cooking time and pH for each food were determined. Duplicate samples of the raw and the cooked foods were dried, ashed, and analyzed for moisture and iron content. Iron content was determined by atomic absorption spectrophotometry. Most of the foods (90%) contained significantly more iron when cooked in iron utensils than when cooked in non-iron utensils. Acidity, moisture content, and cooking time of food significantly affected the iron content of food cooked in iron utensils. Perhaps because of differing amounts of previous use, cooking in different iron skillets resulted in some variation in the iron content of food.
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PMID:Iron content of food cooked in iron utensils. 372 54

The binding of thiol, thiolate, thioether, and disulfide sulfur donor ligands to ferric cytochrome P-450-CAM and myoglobin has been investigated by UV-visible absorption, magnetic circular dichroism (MCD), and EPR spectroscopy. For ferric P-450, the binding of all sulfur donors is competitive with substrate binding. Addition of thiols to P-450 leads to interconvertible thiol or thiolate-bound species depending on the thiol acidity (pKa) and the solution ph; ligation of thiols lowers their pKa by about 4 units. In contrast, only the thiolate-bound form is seen for myoglobin regardless of thiol acidity or solution pH (5.5-11.0), indicating that the heme iron of myoglobin is less electron-rich than that of P-450. Thiolate ligands show much higher affinity (Kd approximately 10(-6) M) for ferric P-450 than do thiols (Kd approximately 10(-3) M). The affinity of thioethers for P-450 (Kd approximately 10(-3) M) is pH-independent (pH 5.5-9.0). The observed disulfide coordination to P-450 represents the first example of disulfide ligation to heme iron; no significant evidence for thioether or disulfide binding to myoglobin is seen. Except for the thiolate complexes, the UV-visible and MCD spectral properties of the other sulfur donor . P-450 complexes are similar to, although distinguishable from, those of native P-450. The ferric P-450 . thiolate complexes exhibit MCD spectra resembling that of ferrous P-450 . CO; both also exhibit unique hyperporphyrin (split Soret) UV-visible spectra. The EPR spectra of all P-450 complexes examined display very narrow spread g-values such as are characteristic of native P-450, indicating that the endogenous cysteinate axial ligand is retained upon complex formation. The dissimilarities observed between P-450 and myoglobin in their reactivity toward sulfur donor ligands at least partly reflect the variation in heme iron electron density resulting from their different endogenous axial ligands and may, in turn, help to explain their respective physiological functions of oxygen activation and reversible oxygen binding.
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PMID:Sulfur donor ligand binding to ferric cytochrome P-450-CAM and myoglobin. Ultraviolet-visible absorption, magnetic circular dichroism, and electron paramagnetic resonance spectroscopic investigation of the complexes. 628 78

The biochemical profile [levels of calcium, phosphorus, magnesium, chlorides and iron, the activities of alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and the concentrations of total protein, albumin, cholesterol, urea, glucose, and vitamins A and E] was studied in the blood serum of 40 anoestrous and 40 control inseminated animals in a production herd with an increased occurrence of anoestrus in gilts. The anoestrous gilts showed significantly lower levels of albumin (P less than 0.01) and glucose (P less than 0.01) and ALP activity (P less than 0.05), and significantly higher concentrations of urea (P less than 0.01), vitamin A (P less than 0.01) and vitamin E (P less than 0.05) and ALT activity (P less than 0.05), as compared with the inseminated controls. An extended enzymatological examination consisting of the evaluation of the activities of ALP, AST, ALT and gamma-glutamyl transferase (GMT) was performed in another set of 22 anoestrous and 20 mated gilts. The anoestrous gilts showed a statistically significant increase in the activities of AST (P less than 0.01), GMT (P less than 0.01) and ALT (P less than 0.05) and an insignificant increase in the activity of ALP in comparison with the control animals. The comparison of the obtained values of the studied biochemical criteria with literary data indicated a lower concentration of magnesium and a higher ALP and ALT activities in the anoestrous and inseminated gilts in both groups under study. A high acidity of fat and a medium to high fungus infestation (Mucor sp., Aspergillus sp.) were found by chemical and mycological examination of the administered feed mixtures. The histological examination of the ovaries of anoestrous animals showed cystically degenerative changes, proliferations of fibrous elements, and partial atrophy of ovarial cortex. It has been inferred from the observations that mycotoxins may be involved in the increase in the occurrence of anoestrus, either by a direct effect on sexual organs or by impairing the function of liver which, secondarily contributes to the rise of ovarial dysfunctions.
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PMID:[Changes in selected biochemical indicators in blood serum in anestrous gilts]. 640 28

In spite of numerous spectroscopic similarities between chloroperoxidase and cytochrome P-450 (P-450) which suggest endogenous cysteinate axial ligation in chloroperoxidase as has been established for P-450, assignment of the endogenous axial ligand of chloroperoxidase has remained controversial since no available free sulfhydryl groups have been detected in chemical studies of chloroperoxidase. To help clarify this problem, we have carried out extensive studies of thiol-binding properties of native ferric chloroperoxidase and have compared our new results with those previously obtained in our laboratory on thiol adducts of P-450. We have found that the ligation of exogenous thiols to the heme iron of chloroperoxidase generates hyperporphyrin (split Soret) spectra (lambda max = approximately 372 and approximately 455 nm), consistent with the formation of bisthiolate low-spin ferric heme adducts as has been established for P-450 and its heme models. However, in contrast to the results with P-450, thiols not only coordinate to the ferric heme iron of chloroperoxidase in the thiolate form in competition with cyanide but also bind, presumably in the thiol form, to a site in the heme vicinity other than the heme iron without competition with cyanide. The thiol acidity (pK alpha greater than 7) or medium pH (pH 3-7) have little effect on the spectral and equilibrium properties of the adducts. Thiol binding to the latter site, which is presumably the organic substrate-binding site, causes a red shift of the Soret peak (339 vector approximately 420 nm) of the high-spin ferric enzyme; the resulting thiol adducts are still predominantly high spin. Similar spectral changes are also observed upon binding of other neutral sulfur (sulfides and disulfide) and oxygen (alcohols and ketones) donor ligands to native ferric chloroperoxidase. In conclusion, the generation of hyperporphyrin spectra upon exogenous thiol binding to native ferric chloroperoxidase provides considerable support for the presence of an endogenous thiolate ligand to the heme of the enzyme in its ferric state.
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PMID:The generation of a hyperporphyrin spectrum upon thiol binding to ferric chloroperoxidase. Further evidence of endogenous thiolate ligation to the ferric enzyme. 654 51

1. The proportion of iron liberated from twenty foods was measured after incubation in vitro with human gastric juice. 2. Results with samples of gastric juice obtained from one subject on ten occasions and from sixteen subjects on a single occasion showed good agreement, the proportions of Fe liberated covering the entire range from zero for egg to 1.0 for wine. 3. Where there was small variations between the results obtained with different samples of gastric juice attempts were made to correlate these with peptic activity, various proteins and mucoproteins, pH and total and free acid; the only factors that varied with the proportions of Fe solubilized were the various measures of acidity. 4. The results correlated highly with those of Layrisse et al. (1969) for the in vivo absorption of Fe from similar foods, although the samples used were not the same. 5. The amounts of Fe solubilized when the foods were mixed with bran, oats or egg (known to reduce the in vivo absorption of Fe from foods) were less than calculated from the sum of each, with two exceptions. 6. The results obtained with human gastric juice differed from those obtained with in vitro methods previously reported, namely treatment with dilute hydrochloric acid and double incubation with pepsin plus HCl at pH 2.5 followed by adjustment to pH 7.5. 7. The amount of Fe solubilized from soya-bean flour by gastric juice was compared with that solubilized by pepsin plus HCl; both systems were shown to be pH dependent but the amount of Fe liberated with pepsin-HCl was much greater than that known to be absorbed by man in invo. 8. It is suggested that incubation with human gastric juice may permit in vitro analyses of 'chemically-available' Fe in foods and thus be of value in food composition tables of terms of 'Fe equivalents' even though the amounts absorbed in vivo are subsequently influenced by other ingredients of the diet and by the Fe status of the individual.
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PMID:Measurement of chemically-available iron in foods by incubation with human gastric juice in vitro. 677 43


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