Gene/Protein Disease Symptom Drug Enzyme Compound
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The biological value of spring wheat Mironovskaya-808 grown with the use of simazine which was introduced into soil prior to appearance of shoots at doses of 0.5 and 1.0 kg/ha has been studied. The organoleptic parameters evaluated by the method of closed testing and physicochemical parameters characterizing the corn quality (protein fat, gluten and ash content, moisture, acidity) of experimental samples did not differ from those of the reference sample. Relative biological value of wheat grown with the use of simazine at a dose of 1.0 kg/ha evaluated by the test-object Tetrahymena pyriformis was slightly higher as compared to the control. The effect of test wheat samples on some parameters of protein metabolism was also studied in male rats (the content of protein and its fractions in blood serum, liver and brain as well as activity of enzymes participating in protein metabolism regulation: aminotransferases of blood serum and liver, catepsins, histidase and tryptophan oxygenase of the liver). According to the data obtained no adverse effect of spring wheat Mironovskaya-808 grown with the use of simazine on the above parameters of protein metabolism was recorded in test animals.
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PMID:[Biological value of Mironovskaya-808 winter wheat grown using the herbicide simazine]. 51 1

Escherichia coli O157:H7 causes hemorrhagic colitis and hemolytic-uremic syndrome in humans, and its major reservoir is healthy cattle. An F-like 92-kb plasmid, pO157, is found in most E. coli O157:H7 clinical isolates, and pO157 shares sequence similarities with plasmids present in other enterohemorrhagic E. coli serotypes. We compared wild-type (WT) E. coli O157:H7 and an isogenic DeltapO157 mutant for (i) growth rates and antibiotic susceptibilities, (ii) survival in environments with various acidity, salt, or heat conditions, (iii) protein expression, and (iv) survival and persistence in cattle following oral challenge. Growth, metabolic reactions, and antibiotic resistance of the DeltapO157 mutant were indistinguishable from those of its complement and the WT. However, in cell competition assays, the WT was more abundant than the DeltapO157 mutant. The DeltapO157 mutant was more resistant to acidic synthetic bovine gastric fluid and bile than the WT. In vivo, the DeltapO157 mutant survived passage through the bovine gastrointestinal tract better than the WT but, interestingly, did not colonize the bovine rectoanal junction mucosa as well as the WT. Many proteins were differentially expressed between the DeltapO157 mutant and the WT. Proteins from whole-cell lysates and membrane fractions of cell lysates were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. Ten differentially expressed approximately 50-kDa proteins were identified by quadrupole-time of flight mass spectrometry and sequence matching with the peptide fragment database. Most of these proteins, including tryptophanase and glutamate decarboxylase isozymes, were related to survival under salvage conditions, and expression was increased by the deletion of pO157. This suggested that the genes on pO157 regulate some chromosomal genes.
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PMID:Characterization of an Escherichia coli O157:H7 plasmid O157 deletion mutant and its survival and persistence in cattle. 1727 24