Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0740441 (acute diarrhea)
 2,275 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The objective of the present investigation is the study and interpretation of the role played by the immunoglobulins, especially IgA, during acute diarrhea in children. IgA, IGG and IgM values in serum and IgA in intestinal secretions were studied in a group of children (between 3 months and 5 years of age) during diarrhea, convalescence and in normals. The method of simple radial immunodiffusion according to Mancini was employed. IgA is the immunoglobulin which suffers the greastest alteration in acute diarrhea. The precipitation halos (the average values), were lower during the diarrhea than in convalescence and in normals.
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PMID:Immunoglobulin in intestinal secretions. 61 59

Twenty-five children with cows' milk protein intolerance were studied. Twenty had presented with an illness clinically indistinguishable from infantile gastroenteritis; an enteropathogenic Escherichia coli was isolated from the stools in two children, and in six another member of the family simultaneously developed acute diarrhoea and vomiting. Twenty-three children had lactose intolerance secondary to cows' milk protein intolerance. Eight out of 20 children were found to be partially IgA deficient. An acute attack of gastroenteritis, in damaging the small mucosa, may act as a triggering mechanism in cows' milk protein intolerance, and a deficiency in IgA may be a predisposing factor in so far as it allows the patient to become sensitised to foreign protein.
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PMID:Cows' milk protein intolerance: a possible association with gastroenteritis, lactose intolerance, and IgA deficiency. 77 36

Rotavirus infection in the Dar es Salaam area of Tanzania was studied in 99 hospitalized children with acute diarrhoea and 99 hospitalized non-diarrhoea referents matched for sex and age. Of the diarrhoea cases 43.4% had rotavirus in the stools as opposed to 15.2% of the referents. The high carrier rate among the referents represents a serious risk of nosocomial transmission. More referents than cases had serum IgG antibodies to rotavirus, 52.5% and 35.4%, respectively (P < 0.02), while there was no correlation with serum IgM and IgA or faecal IgA antibodies. The latex agglutination test had a sensitivity comparable to that of electron microscopy (100%) and a specificity of 93.8%. The Slidex test appeared to be superior to the Rotalex test in that it gives very few false-positive reactions. The SDS-PAGE patterns of 11 RNA segments were compatible with the presence of group A strains with considerable heterogeneity among the strains. Symptoms and signs and some environmental data were recorded. None of them was clearly associated with rotavirus infection among the diarrhoea cases. It is concluded that rotavirus is a major cause of acute infectious diarrhoea in Tanzania.
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PMID:Rotavirus infection in Tanzania: a virological, epidemiological and clinical study among young children. 132 4

Intestinal secretory IgA (sIgA) response or lack of response among adults in Mexico with diarrhea was used as an indicator of enteropathogenicity of Aeromonas species and Plesiomonas shigelloides. sIgA was extracted from stool specimens obtained at day of presentation and 5 days later. Total sIgA was standardized, and specific sIgA titer against the organism being shed by each patient was determined. Western blotting was used to determine which microbial antigens elicited an intestinal sIgA response. Of 12 subjects shedding Aeromonas sobria or Aeromonas hydrophila, 11 had a fourfold or greater sIgA titer rise against the infecting strain. Western blotting showed that somatic lipopolysaccharides were the immunodominant antigens. No sIgA titer rises were detected among two patients shedding Aeromonas caviae or in 14 shedding P. shigelloides. This study provides further evidence of the significance of A. sobria and A. hydrophila as pathogens in acute diarrhea but raises additional questions about the role of P. shigelloides, at least in US adults with travelers' diarrhea.
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PMID:Intestinal secretory immune response to infection with Aeromonas species and Plesiomonas shigelloides among students from the United States in Mexico. 194 Apr 78

Cryptosporidium was detected in 2 (1.5%) out of 132 children under 2 years with acute diarrhea; in 2 (3.2%) out of 63 patients under 2 years with persistent diarrhea; in 1 (3.9%) out of 26 malnourished patients younger than 2 years with an episode of acute diarrhea and in 7 (1.4%) out of 516 pediatric ambulatory patients who consulted for acute or chronic diarrhea or recurrent abdominal pain. The clinical histories of the 5 infants with cryptosporidiosis who belonged to the first 3 studies, are presented. All they had prolonged diarrhea (more than 15 days long), and one of them showed low IgG and IgA serum concentrations, but normal proportions of T lymphocyte populations.
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PMID:[Cryptosporidiosis: studies in children in communities of low socioeconomic level]. 208 94

In 233 patients with acute diarrhoea in paired sera Campylobacter antibodies classes IgG and IgA were assessed by the ELISA method. As antigen the external membrane protein of the strain Campylobacter jejuni was used. Raised IgG levels (greater than 50 u.) and/or IgA (greater than 80 u.) were found in 15% of all examined patients. A quadruple increase of values in one or both classes was recorded in 12.4% of the patients. The incidence of antibodies against Campylobacter jejuni provides evidence that this infectious agent is frequent in this country. Antibodies class IgA suggest by their dynamics acute contact with the Campylobacter antigen. On the other hand, IgG antibodies are not of major importance in newly diagnosed disease.
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PMID:[Antibodies to Campylobacter jejuni in patients with acute enteritis]. 279 Sep 81

The development of mucosal immunity is presumed to be the most important marker of rotavirus infection. The practical difficulties of obtaining small-bowel secretions stimulated this study of the antibody response to acute rotavirus infection at other sites. Forty-four infants admitted to the hospital with rotavirus gastroenteritis had serum, saliva, and feces collected at the acute phase (median, 5.5 days), during convalescence (median, 33.5 days), and 4 months later (median, 12.2 weeks). A subgroup of 19 children also had duodenal juice collected in parallel. Rotavirus-specific immunoglobulin G (IgG), IgA, secretory immunoglobulin, and IgM were measured and compared in all samples. The results showed that the estimation of antirotavirus serum IgM, serum IgG, duodenal juice IgA, and duodenal juice IgM by an enzyme immunoassay indicated an immune response to severe primary rotavirus infection in all children. Four months later, the levels of serum IgG and IgA served as the most sensitive markers of the preceding rotavirus infection. The predictive accuracies of immune responses at different sites in relation to a positive IgA immune response in the duodenum were calculated. Fecal IgA predicted duodenal IgA rotavirus antibodies with accuracies of 86% at 1 month and 92% at 4 months. The high sensitivity of serum IgM and IgG in detecting rotavirus infection and the high predictive accuracy of fecal IgA as an indicator of duodenal IgA abrogates the need for duodenal intubation to detect (or monitor) an immune response to rotavirus infection. This finding has important practical implications for epidemiological studies of acute diarrhea in children and in rotavirus vaccine trials.
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PMID:Comparison of serum and mucosal antibody responses following severe acute rotavirus gastroenteritis in young children. 283 91

We studied the immune response to acute diarrhea by examining antibody-secreting cells among peripheral blood lymphocytes, which are believed to be derived from the intestinal mucosa and to be on their way back there. In 23 of 24 patients, a dramatic increase in the total number of cells actively secreting immunoglobulins was detected one week after onset of diarrhea, and most of the cells were secreting IgA. Cells secreting antibody specific to the pathogen (Campylobacter jejuni or Salmonella spp.) also appeared at this time but accounted for only a part of the total response. The data suggest that diarrhea induces a vigorous, apparently polyclonal response, including antibodies to normal intestinal flora. The response to the infective agent was outstanding and suggests that this method can be used to identify the causative agent of an infection.
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PMID:Immune response to acute diarrhea seen as circulating antibody-secreting cells. 305 18

It is obvious from this conference that although there are many more unresolved problems and questions than answers, the definition of the problems associated with pediatric diarrhea which face the medical/scientific community has been painstakingly, persistently, and frequently brilliantly illuminated by the efforts of many investigators. The presentations in this session will address and define areas for future productive investigation, particularly as they relate to the specific challenges of early feeding, nutrition, immunobiology, and the application of present knowledge to underdeveloped areas of the world. The lack of effectiveness of the many vaccines available for prophylaxis against gastrointestinal infection illustrates the need for fundamental research into the immunobiology of the intestine. It is apparent that humoral immunoglobulin cannot adequately forecast resistance to enteric challenge. Yet, while the newly appreciated role of surface IgA as a protector of intestinal surfaces against enteric invasion significantly updates understanding of intestinal immunology, it fails to adequately explain observations on the behavior and function of either the cellular elements of the intestinal immune mechanism or the function of other immunoglobulins present in the intestine. Dr. Harry R. Hill will discuss immunologic factors associated with the gastrointestinal tract. Dr. Jose Santos will present an update on one of the more complex issues associated with pediatric diarrhea to arise during the last decade, i.e., the influence of nutrition and the various states of malnutrition on the severity and outcome of diarrheal disease. Dr. Richard Guerrant will review the most serious problems in diarrheal control and research to be faced in the next decade. Areas of concern include the impact of polymicrobial diarrheal disease on morbidity, chronic vs. acute diarrhea, new etiologies of diarrheal disease, and the intraepidemic epidemiology of etiologic agents. Dr. John M. Masten will conclude the conference with a synopsis of the presentations.
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PMID:Research in pediatric diarrhea. 308 Jul 40

In 70 children 0-4 years of age with acute diarrhoea, the shedding of rotavirus and the excretion of rotavirus-specific IgA antibody in the stools were examined throughout the period of clinical symptoms. Quantitative detection of rotavirus and IgA was performed by an ELISA technique. The maximal rotavirus shedding was found between the second and fifth day and the maximal excretion of IgA antibody about the seventh day, which marked the clinical recovery of most children. Throat swabs were examined for both virus particles and specific IgA antibody to try to document the respiratory spread of rotavirus infection. Rotavirus antigen could not be demonstrated in the throat swabs, but specific IgA antibody was detected at levels comparable to the faecal specimens obtained at clinical recovery. The observations indicate that the presence of rotavirus secretory IgA limits the duration of diarrhoea and plays a major role in the intestinal resistance to infection.
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PMID:Faecal and pharyngeal shedding of rotavirus and rotavirus IgA in children with diarrhoea. 609 2


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