Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0740441 (acute diarrhea)
 2,275 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Five selective media were evaluated for their effectiveness in the primary isolation of Aeromonas spp. from human patients with acute diarrhea and from healthy domestic animals. Sheep blood agar with 30 mg of ampicillin per liter (ASBA30) yielded a significantly higher percentage of positive specimens as compared with the four other media. The effective combination of two selective media with which 98% of all isolates were detected and with which all of the three human-associated Aeromonas spp. could be isolated was ASBA30-DNase-toluidine blue agar. ASBA30 was the most sensitive medium since it permitted more growth of Aeromonas colonies and effectively suppressed competing microflora. We recommend the use of ASBA30-DNase-toluidine blue agar for investigations in which an attempt is made to assess the significance of Aeromonas spp. in the etiology of human diarrhea.
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PMID:Comparison of selective media for primary isolation of Aeromonas species from human and animal feces. 369 36

The present study describes detection of picobirnavirus (PBV) in faecal samples from bovine and buffalo calves employing the polyacrylamide gel electrophoresis (PAGE). A total of 136 faecal samples from buffalo (n = 122) and cow calves (n = 14) exhibiting clinical signs of diarrhoea and from healthy calves were collected during 2007-2010 from subtropical (central India) and tarai area of western temperate Himalayan foothills (Uttarakhand). The dsRNA nature of the virus was confirmed by nuclease treatment (RNase A, RNaseT1 and DNase 1). PAGE results confirmed 3.67% (5/136) positivity for PBV, showing a typical genomic migration pattern with two discrete bands with size of approximately 2.4 and 1.7 kbps for the larger and smaller segments, respectively. Among the five PBV samples identified, three were from buffalo calves and one from cow calf exhibiting clinical signs of acute diarrhoea, while one sample from non-diarrhoeic buffalo calf also showed the presence of PBV. None of the samples showed dual infection of rotavirus and PBV. The preliminary findings indicate sporadic incidences of PBV in bovine calves and emphasize the need for the development of better diagnostics for early detection and genetic characterization of these emerging isolates of farm animals of economic significance.
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PMID:Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India. 2147 44