Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0740441 (acute diarrhea)
 2,275 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In vitro uptake of 14C-labelled amino acids by segments of small intestine was determined in sucking (2-4-d-old) Wistar rats. Intragastric injections of heat-stable (ST) toxin of enterotoxigenic Escherichia coli (ETEC) were given to produce fluid accumulation, defined as a gut weight: carcass weight value of > 0.085. Continued active uptake of the prototypic amino acids, leucine (by active transport system 1 for monoamino monocarboxylic (neutral) amino acids), lysine (by active transport system 2 for dibasic amino acids), and proline (by active transport system 3 for N-substituted amino acids), persisted during the active fluid accumulation response to ETEC ST toxin. The mean Kt and mean Vmax of the amino acid transport systems were similar in control (non-injected) and ST toxin-injected rats. The present study provides a scientific basis for the use of amino acids in oral rehydration solutions utilizing amino acid transport systems which are linked to absorption of Na (and water) so that reduction in diarrhoeal stools can be achieved, and emphasizes the importance of maintaining feeding during acute diarrhoea to prevent development of malnutrition.
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PMID:The effect of Escherichia coli heat-stable (STA) enterotoxin on in vitro uptake of amino acids by small intestine of sucking rats during fluid accumulation. 832 51

Enteroinvasive Escherichia coli (EIEC) share important features with Shigella spp., but EIEC strains are difficult to identify because their biochemical reactions are variable, and Sereny tests or other biological and molecular assays are expensive or hard to perform. The aim of this work was to detect probable enteroinvasive E. coli strains by using four biochemical tests, in children under 5 years of age with and without acute diarrhea. 330 strains of E. coli isolated from children with diarrhea, and 660 strains from children without diarrhea were studied. All strains were tested with the following tests: mucus , lysine and ornithine decarboxylase and motility. The strains which were negative to the four tests were tested by Sereny assay. Twelve strains (3.6%) isolated from children with diarrhea were negative to the tests proposed; eleven were lactose positive and only one was lactose negative. Three strains (0.5%) from children without diarrhea were negative to the tests proposed and were lactose positive. All the 15 strains (100%) were positive in Sereny assay. We recommend the use of these four biochemical tests for initial detection of EIEC strains, because their cost is very low and it is feasible carry out them in small diagnostic laboratories.
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PMID:Four biochemical tests for identification of probable enteroinvasive Escherichia coli strains. 1093 66