Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0699790 (
colon cancer
)
28,837
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We previously reported that the over-expression of
hRFI
, a protein preferentially expressed in the digestive tract regions of several cancers, exhibited a tendency to inhibit TNF-alpha induced apoptosis. In this study, we sought to determine the potential effect of
hRFI
expression on the sensitivity to 5-fluorouracil (5-FU) and/or other fluoropyrimidines. For the whole lysates of 8
colon cancer
cell lines, we performed Western blotting with anti-
hRFI
antibody and analyzed the correlations between the expression level of
hRFI
and the cell lines' sensitivity to 5-FU induced apoptosis. Furthermore, for a tissue microarray consisting of 32 xenograft derived human cancer cell lines, we examined the expression levels of
hRFI
and survivin by immunohistochemical staining, and analyzed the correlations between the expression of each protein and the sensitivity to several chemotherapeutic agents in the xenografts examined. Both in
colon cancer
cell lines and in xenografts, the expression level of
hRFI
was correlated with resistance to 5-FU and its derivatives. This evidence suggests that
hRFI
may be a marker predicting the response to fluorouracil derived chemotherapeutic agents and that the reduction of the expression level of
hRFI
might improve the outcome of chemotherapy.
...
PMID:High hRFI expression correlates with resistance to fluoropyrimidines in human colon cancer cell lines and in xenografts. 1627 May 26
