Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0699790 (
colon cancer
)
28,837
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
G protein-coupled receptors (GPRs) are highly related to oncogenesis and cancer metastasis.
G protein-coupled receptor 137
(
GPR137
) was initially reported as a novel orphan GPR about 10 years ago. Some orphan GPRs have been implicated in human cancers. The aim of this study is to investigate the role of
GPR137
in human
colon cancer
. Expression levels of GRP137 were analyzed in different
colon cancer
cell lines by quantitative polymerase chain reaction and western blot analysis. Lentivirus-mediated short hairpin RNA was specifically designed to knock down
GPR137
expression in
colon cancer
cells. Cell viability was measured by methylthiazoletetrazolium and colony formation assays. In addition, cell cycle characteristic was investigated by flow cytometry. GRP137 expression was observed in all seven
colon cancer
cell lines at different levels. The mRNA and protein levels of
GPR137
were down-regulated in both HCT116 and RKO cells after lentivirus infection. Lentivirus-mediated silencing of
GPR137
reduced the proliferation rate and colonies numbers. Knockdown of
GPR137
in both cell lines led to cell cycle arrest in the G0/G1 phase. These results indicated that
GPR137
plays an important role in
colon cancer
cell proliferation. A better understanding of
GPR137
's effects on signal transduction pathways in
colon cancer
cells may provide insights into the novel gene therapy of
colon cancer
.
...
PMID:Down-regulation of GPR137 expression inhibits proliferation of colon cancer cells. 2972 88
