Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0699790 (colon cancer)
 28,837 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Biliary glycoprotein (BGP), also known as C-CAM-1, has been shown to be down-regulated in colon and prostate tumors. Previously, we demonstrated that BGP mRNA is up-regulated by interferon-gamma (IFN-gamma) in colon cancer cell lines (Takahashi, H., Okai, Y., Paxton, R. J., Hefta, L. J. F., and Shively, J. E. (1993) Cancer Res. 53, 1612-1619). We now show that the BGP promoter contains an interferon-sensitive response element (ISRE) that is specifically protected in in vivo footprints. Interferon regulatory factor-1 (IRF-1) was identified as the ISRE-binding factor by electrophoretic mobility shift assays. The induction of IRF-1 mRNA by IFN-gamma in HT-29 cells reaches a maximum at 6 h and is superinduced by cycloheximide. Four mRNA species for BGP are induced by IFN-gamma, the major band of which is inhibited by cycloheximide. Transfection of HT-29 cells with an IRF-1 expression plasmid (pAct-1) transactivates a BGP promoter reporter gene containing wild-type (but not mutant) ISRE. Electrophoretic mobility shift assay analysis of a second footprint reveals the binding of Sp1, an Sp1-like protein, and upstream stimulatory factor. The Sp1-like complex was also induced by IFN-gamma treatment of HT-29 cells and may be a second point of transcriptional control for the BGP gene.
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PMID:Role of interferon regulatory factor-1 in the induction of biliary glycoprotein (cell CAM-1) by interferon-gamma. 891 Apr 34

Biliary glycoprotein (BGP, CD66a, or C-CAM-1) is a cell adhesion glycoprotein expressed in colon, liver, and hematopoietic tissues. Four major isoforms (a-d) of BGP are expressed in most epithelial tissues by alternative mRNA splicing from a single gene. Since BGP is down regulated in colon cancer and in premalignant colonic adenomas, it has been of interest to study its expression in other tumors. Using immunohistochemistry with a BGP specific antibody, and mRNA analysis by in situ hybridization, RNase protection, and RT-PCR, we show here that BGP is expressed to the same extent in both normal and malignant breast, demonstrating that BGP is not down regulated in breast cancer. In normal breast, BGP expression is confined to the apical surface of ductal and lobular epithelial cells, while in invasive carcinoma of the breast, BGP is expressed throughout the cytoplasm. In situ hybridization shows a specific pattern of BGP expression in both normal and malignant breast epithelium. RNase protection analysis confirms the immunohistochemistry results and shows no quantitative differences between normal and malignant breast. RT-PCR analysis agrees with these results and shows that only 3 of the 4 major isoforms (a, c, d) of BGP are expressed in normal and malignant breast. Since recent studies by Turbide et al (Cancer Res 57: 2781-2788, 1997) have shown that the ratio of murine BGP isoforms may affect tumor suppression in colonic cancer, it is proposed here that the isoform difference between human breast and colon may account for the observed lack of BGP down-regulation in breast vs colon cancer.
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PMID:Expression of biliary glycoprotein (CD66a) in normal and malignant breast epithelial cells. 985 84