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Query: UMLS:C0684249 (lung carcinoma)
 23,830 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In 1984, the author first developed a simple, quick, non-invasive, economical method of detecting cancer in specific internal organs, using the Bi-Digital O-Ring Test (BDORT), with a microscope slide of specific cancer of a specific internal organ as a reference control substance. The detection rate for cancer screening was much greater than with any standard diagnostic tests. When imaging was performed using the BDORT, the area of positive response to the cancer positive slide was often much greater than the actual size of the cancer itself. This was due to the fact that most of the cancer tissue of the lungs or digestive system contained viruses such as HTLV-3 (often found in adenocarcinoma of the lung, stomach, head of pancreas, and colon) or HTLV-1 (often found in small-cell carcinoma of the lung and certain types of leukemia). The extent of the virus positive area was often far greater than that of the cancer tissue itself and was distributed in a much greater area surrounding the cancer. For this reason, the virus alone showed a response which could be mistaken for cancer tissue. The author succeeded in differentiating the exact location of cancer tissue itself from surrounding cancer related virus (with or without other microbes) positive area by using a pair of identical microscope slides with the same cancer tissue. One of the slides was exposed to ultra-violet rays (peak wavelength of 253.7 nm mercury vapor atomic resonance spectral line) for 40 seconds-4 minutes. After this exposure, the BDORT response to the virus (with or without other microbes) associated with the cancer tissue was completely eliminated, while the response to the cancer tissue was maintained. Using an ultraviolet exposed cancer slide, the imaging of the part of the body which responded to this virus-free cancer slide indicated the actual location of the cancer tissue, which was often confirmed by standard X-ray or other imaging methods when the thickness of the tumor was relatively large. These cancers detectable by standard laboratory tests had strikingly weakening response to the BDORT (-3.5 and -4), with ultra-violet exposed cancer slide as well as for antibody of Oncogen C-fos. The smallest size of cancer tissue detected by this method was less than 1mm in diameter in the very early stage of the cancer, which usually cannot be detected by current laboratory tests.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Simple non-invasive early detection and localization of specific cancer tissues of internal organs and differentiation of cancer tissue from surrounding areas infected by cancer related viruses, as well as evaluation of their micro-circulatory condition & drug uptake using the BI-Digital O-Ring Test. 198 44

The tumor targeting properties of murine monoclonal antibodies (MAbs) generated in our laboratory against non-small cell carcinoma of the lung have been investigated in nude mouse xenograft models. The MAbs selected for evaluation, RS5-4H6, RS7-3G11, and R511-51, have pancarcinoma reactivity, as shown by immunoperoxidase staining of the majority of tumors from the lung as well as breast, colon, kidney, and ovary. The localization of the three MAbs which bind to distinct antigens, and exhibit different levels of cross-reactivity with normal human epithelial tissues, are compared. The MAbs are of the IgG1 isotype. Since these MAbs were reactive with Calu-3, a human adenocarcinoma of the lung cell line grown as xenografts in nude mice, this system was selected as our initial tumor target. The MAbs were found to localize preferentially to the heterotransplanted tumors, with from 6.6 to 8.6% of the injected dose per gram accreting in the tumor at 7 days. Tumor/nontumor ratios of up to 9.7 were seen with one MAb at day 14. The targeting of MAb RS11-51 and F(ab')2 fragments of RS11-51 in GW-39, a human colon cancer grown in nude mice, was also studied. Accretion of intact RS11-51 and F(ab')2 fragments into GW-39 was greatly increased compared to Calu-3. In view of the high frequency of antigen expression on a wide variety of tumors, and the ability to target in vivo, these new MAbs may have potential use in the imaging and therapy of cancer.
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PMID:Murine monoclonal antibodies raised against human non-small cell carcinoma of the lung: specificity and tumor targeting. 215 58

The fine-needle aspiration (FNA) cytology of two cases of papillary adenocarcinoma of the lung is reported. Both cases showed psammoma bodies and papillary clusters of tumor cells in FNA specimens. Both tumors were resected and confirmed as primary papillary carcinoma of the lung by histologic examination and by clinicopathologic exclusion of the possibility of metastasis from other organs.
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PMID:Psammoma bodies in fine-needle aspiration cytology of papillary adenocarcinoma of the lung. 220 53

This paper describes an immunoglobulin G1 mouse monoclonal antibody (MCA) 44-3A6 directed against a human adenocarcinoma of the lung, cell line A549. This hybrid is a fusion product of the mouse myeloma SP 2/0.Ag14 and spleen cells from a BALB/c mouse which had been hyperimmunized with A549. Live cell radioimmunoassays, immunofluorescences, and fluorescent activated cell sorter analysis indicate that MCA 44-3A6 reacts with a cell surface antigen. Western blot analysis identifies a major antigen band with the apparent molecular weight of 40,000. Enzyme treatment of A549 target plates shows that the antigen is sensitive to proteases. This MCA does not react with carcinoembryonic antigen. Patients having a variety of different lung carcinomas do not appear to have detectable antigen in their serum, nor does the antigen appear to be shed into culture supernatants by human lung carcinoma cell lines. The antigen is preserved in formalin-fixed, paraffin-embedded tissue sections and shows a cell surface and/or cytoplasmic staining pattern. Immunohistochemical staining of various bronchopulmonary carcinomas demonstrated binding to be restricted to tumors with features of "glandular" differentiation. This MCA may have clinical and diagnostic utility due to its selective binding for a subset of carcinomas of the lung.
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PMID:Monoclonal antibody 44-3A6 as a probe for a novel antigen found on human lung carcinomas with glandular differentiation. 241 99

A previously described epitope (designated 43-9F) with high specificity for human squamous carcinoma and adenocarcinoma of the lung is here reported to be associated with the proliferative and tumorigenic activity of a cloned human squamous lung carcinoma (SLC) cell line (SLC-L11) and a number of cloned sublines. Lines with appreciable 43-9F epitope density (43-9F+) had a short population doubling time and were tumorigenic in athymic nude mice, whereas clones with low 43-9F epitope density (43-9F-) of the same line had longer population doubling times and did not result in 43-9F- tumors in athymic nude mice. Some of the 43-9F- clones reverted to the 43-9F+ phenotype and then became tumorigenic. Two other SLC lines (SLC-L12 and SLC-L13), positive and negative, respectively, for 43-9F also expressed tumorigenic and proliferative behavior concordant with the data for SLC-L11 and its sublines. Cell lines of human squamous cell carcinomas had a pronounced intratumoral heterogeneity in respect to 43-9F epitope expression. Fluorescence-activated cell sorting of cells with high and low 43-9F density and subsequent cloning demonstrated that the cloning capacity was confined almost exclusively to cells with high antigen density. Immunoaffinity chromatography and gel filtration showed that the epitope was expressed in glycoproteins with molecular weights in the range of 5 X 10(6)-5 X 10(4). Mild alkaline hydrolysis reduced the binding of 43-9F antibody to SLC-glycoproteins while neuraminidase treatment augmented the 43-9F antibody binding. It is concluded that expression of 43-9F+ glycoproteins seems linked to the proliferative and tumorigenic features of human squamous lung carcinoma cell lines.
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PMID:Association between expression of a tumor-associated carbohydrate epitope and the proliferative and tumorigenic activity of a human squamous lung cancer cell line and epitope-positive and -negative sublines. 244 77

Murine monoclonal antibodies (MAbs) reactive with human non-small cell carcinoma of the lung (NSCCL) were produced following immunization with a membrane preparation of Calu-3, a human adenocarcinoma of the lung cell line grown in nude mice. Positive hybrids unreactive with normal liver membranes and human peripheral white blood cells were selected for further testing. One MAb (RS5-4H6) recognized an antigen expressed in a variety of lung and other carcinoma cell lines as detected by flow cytometry. Immunohistology showed a selectivity for normal and neoplastic lung epithelium, as well as other cancers. The antigen was detected by immunohistology in 87% of tumor specimens derived from the lung, breast, colon, kidney, and ovary. Most other normal human tissues were not stained but occasional cells of the stomach, salivary and other glands, as well as kidney tubules were reactive. This MAb is an IgG1. Western blot analysis indicated that the antibody reacts selectively with an antigen greater than 300 kD. The antigen is resistant to neuraminidase treatment and periodate oxidation, but sensitive to pronase treatment, suggesting that the epitope is peptide in nature. This antibody may be potentially useful as a targeting agent for radioimmunodetection and immunoconjugate therapy.
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PMID:A murine monoclonal antibody raised against human non-small cell carcinoma of the lung. 285 33

A clinical phase I-II evaluation of 2-amino-1,3,4-thiadiazole (A-TDA) administered daily, twice a week, or weekly was undertaken, in which 71 patients were treated with a range of doses from 2 mg/m2 to 200 mg/m2. Pharmacokinetic studies employing high-performance liquid chromatography (HPLC) demonstrated a terminal (beta) serum half-life of 2.19 h. Stomatitis, dermatitis, nausea, vomiting, and lethargy were observed. No significant leukopenia or thrombocytopenia, however, was noted. A-TDA administration led to hyperuricemia, which was adequately controlled with concurrent administration of allopurinol. Antitumor responses included one partial response in a patient with large cell carcinoma of the lung and three objective responses (2 non-small cell lung and 1 squamous cell carcinoma of the esophagus). Two patients with adenocarcinoma of the lung had a marked improvement of psoriasis during A-TDA therapy. Further phase II studies in patients with cancer and trials in patients with psoriasis are recommended.
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PMID:Clinical and clinical pharmacologic studies of 2-amino-1,3,4-thiadiazole (A-TDA:NSC 4728). 293 41

The aims of this study were to develop a protocol for the identification and enrichment of cancer cells from sputum obtained from patients with adenocarcinoma of the lung (n = 6) and large-cell undifferentiated carcinoma of the lung (n = 2), and to compare these findings with the results from our previous studies on other cell types from lung cancer. The hypotheses tested were: Cancer cells in sputum can be preserved following flow sorting. Enrichment for cancer cells from acridine orange (AO)-stained specimens can be achieved. Discrimination of cancer cells from noncancer cells is by AO green fluorescence and discrimination of lymphocytes from other cell types is by AO red fluorescence. Cancer cells are consistently enriched in the AO high green and red fluorescence region, although, for a given cell type, maximal enrichment is patient-dependent. Finally, cancer cell enrichment and lymphocyte exclusion can be done simultaneously. Cells from sputum were initially fixed, stained with AO, sorted on a dual parameter flow sorter, and classified into six groups corresponding to two ranges of green and three ranges of red fluorescence intensities. Cells of each region were stained by the method of Papanicolaou and differential counts were performed to determine the relative frequencies (i.e., purities) of leukocytes, macrophages, squamous cells, and cancer cells, in sorted and unsorted (i.e., control) samples. The average purity of leukocytes (81%), macrophages (6%), squamous cells (11%), and cancer cells (2%) varied markedly from sample to sample. However, the largest enrichment values (i.e., ratio of purity of a cell type in a sorted sample to its purity in the unsorted control sample) achieved for cancer cells consistently occurred for each patient sample in the region corresponding to high green and high red fluorescence intensities. Experimentally, a cancer cell average enrichment of sixteen-fold was obtained by this method. Additionally, fluorescence intensity ranges which increased the enrichment for macrophages by cell sorting typically excluded leukocytes and squamous cells, and vice versa. Finally, red fluorescence intensity was the primary discriminatory parameter for all cell types studied, although the additional use of green fluorescence intensity significantly increased cancer cell enrichment rates.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Automatic cell identification and enrichment in lung cancer: V. Adenocarcinoma and large cell undifferentiated carcinoma. 298 59

Cardiac tamponade due to malignant effusion, though rarely the initial manifestation of malignancy, is usually secondary to adenocarcinoma of the lung. Two cases are reported. One patient presented with cardiac tamponade; the other had diffuse cutaneous involvement of the left neck and shoulder two months before he presented with cardiac tamponade. Cytologic examination of both fluids revealed adenocarcinoma. Ultrastructural examination showed poorly differentiated adenocarcinoma in the first patient and bronchioloalveolar carcinoma in the second; carcinoembryonic antigen levels in the fluids were 9.4 ng/mL and over 60 ng/mL, respectively. The computed tomographic (CT) scans of both patients revealed mediastinal fullness with no lung involvement. Even in the absence of a pulmonary mass, lung carcinoma may be the likely primary in patients with malignant pericardial effusions.
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PMID:Malignant pericardial effusion and cardiac tamponade. 302 32

The staging and histologic cell type of patients in the Lung Cancer Study Group (LCSG) clinical trials program are reviewed and confirmed or resolved at the reference center for anatomic and pathologic classification of lung cancer. A high level of consistency in classification has been achieved through the use of criteria that minimize intraobserver variability. The data obtained from the review project have been used to characterize the relationship of disease extent and cell type to survival in the clinical trials population. Survival characteristics were generated for 1,121 patients who underwent apparent complete resection of nonsmall cell lung cancer and were subsequently entered into various protocols to receive either adjuvant treatment or no further therapy. The end results study provides some insight regarding the biological behavior of squamous cell carcinoma and adenocarcinoma of the lung in terms of the anatomic extent of disease at the time of apparent complete resection. Patients with squamous cell carcinoma had an outcome superior to that of patients with adenocarcinoma in every TNM subset. The differences in survival according to these major cell types were significant overall and in the T1 N0, T1 N1, and T2 N1 subsets but not in the TNM subsets in stage III disease. Histologic cell type and extent of disease are important factors in survival expectations; thus the accuracy and reproducibility of these classifications plays a significant role in the evaluation of differing modalities of treatment.
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PMID:Lung cancer classification: the relationship of disease extent and cell type to survival in a clinical trials population. 303 95


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