Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0679427 (myeloblastosis)
 982 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A three-step sandwich enzyme-linked immunosorbent assay was developed for the detection of avian oncovirus group-specific (gs) antigens. The assay procedure was to coat the wells of microtitre plates with hamster anti-gs IgG, react with crude or purified antigen and finally with hamster anti-gs IgG linked to horseradish peroxidase. The sensitivity was 8 picograms (pg) of input avian myeloblastosis virus (AMV) protein, with negligible background. As the ELISA takes less than 2 h to perform, large-scale screening for infected birds is feasible. A blocking assay was also developed for detecting anti-gs antibodies by adding unlabelled antiserrum after the antigen step.
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PMID:Detection of avian oncovirus group-specific antigens by the enzyme-linked immunosorbent assay. 624 72

Conjugates of horseradish peroxidase with rabbit IgG antibody against gs antigen (p27) of avian myeloblastosis virus were prepared using glutaraldehyde and periodate methods of coupling. Conjugates were evaluated for the detection of gs antigen of avian leukosis and sarcoma viruses in the ELISA system and the periodate conjugate was found to be superior. With an ELISA based on a periodate conjugate it was possible to detect 5 x 10(3) IU of leukosis virus propagated in cell culture. Comparisons between the PM test and ELISA on biological samples showed the PM test to be 2 to 2,000 times more sensitive than ELISA for vaginal swabs and embryo extracts. ELISA was 16 to 64 times more sensitive than the CF test when comparisons were made on albumens and embryo extracts. When the ELISA was used for testing vaginal swabs for the prevalence of LLV infection in commercial laying flocks, it appeared that the reliability of ELISA varied. In all five flocks studied, ELISA detected a higher percentage of gs+ [virus+] hens than did the PM test, the proportion of ELISA(+) PM(-) samples ranging from 3 to 53%. In some flocks ELISA failed to detect a low proportion [ 1 to 5%] of infected hens when vaginal swabs were used for screening. The suitability of ELISA for use in screening of commercial flocks for LLV infection is discussed.
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PMID:Detection of avian leukosis virus with the ELISA system: evaluation of conjugation methodology and comparison of sensitivity with the phenotypic mixing test in commercial layer flocks. 1877 Feb 24

Increasing evidence suggests that long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) have roles during biotic and abiotic stress, though their exact contributions remain unclear. To explore their biological functions in response to chilling in bell pepper, we examined their accumulation profiles by deep sequencing and identified 380 lncRNAs, 36 circRNAs, 18 miRNAs, and 4128 differentially expressed mRNAs in the chilled versus the non-chilled fruit. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed differentially expressed genes and putative ncRNA targets, including transcription factors of multiple classes, such as myeloblastosis (MYB), basic helix-loop-helix (bHLH), and ethylene response factor (ERF) transcription factors (TFs), enzymes involved in bio-oxidation and oxidative phosphorylation (serine/threonine-protein kinase, polyphenol oxidase, catalase, peroxidase, lipoxygenase, and ATPase), and cell wall metabolism-related enzymes (beta-galactosidase, pectate lyase, pectinesterase, and polygalacturonase). On the basis of the accumulation profiles, a network of putatively interacting RNAs associated with bell pepper chilling was developed, which pointed to ncRNAs that could provide the foundation for further developing a more refined understanding of the molecular response to chilling injury.
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PMID:Analysis of the Coding and Non-Coding RNA Transcriptomes in Response to Bell Pepper Chilling. 2998 49