Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0679427 (myeloblastosis)
 982 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

3'-Azido-2',3'-dideoxythymidine (az-T) inhibited effectively the reproduction of some retroviruses; among these viruses were the four serological subgroups of sarcoma Raus virus in chicken embryo, avian myeloblastosis virus and erythroblastosis virus in chicken. This inhibition was specific towards retroviruses and practically was not observed in the case of infections DNA- and RNA-genome model viruses of vaccinia and influenza, at whose reproduction reverse transcriptase is not involved. Three other 3'-modified nucleosides did not block the above-listed retroviruses. For chickens, az-T showed low toxicity. The molecular mechanisms of the action of az-T are discussed.
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PMID:[The effect of 3'-azido-2',3'-dideoxythymidine on experimental viral infections]. 282 79

The purification procedure for quantitative recovery of the three molecular forms, alpha, alpha beta and beta 2 of avian reverse transcriptase (Ueno, A., Ishihama, A. and Toyoshima, K. (1982) J. Biochem. 91, 311-322) was improved with respect to removal of nucleases. The three enzyme forms were prepared from avian myeloblastosis virus by CsCl centrifugation and poly(G)-agarose column chromatography. The alpha- and alpha beta-forms of the enzyme were further purified to near homogeneity by column chromatography on heparin agarose and DNA cellulose, respectively. The three enzyme forms thus purified were equally active in influenza virus RNA-directed synthesis of single-strand cDNA. By contrast, the alpha-form enzyme was more active in the single-strand cDNA-directed synthesis of double-strand DNA than the other two enzyme forms.
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PMID:Improved purification and enzymatic properties of three forms of reverse transcriptase from avian myeloblastosis virus. 608 85

The capacity of influenza virion 4S RNA for serving as low-molecular primer has been studied in RNA-directed DNA synthesis catalyzed in vitro by reverse transcriptase of avian myeloblastosis virus. 4S RNA purified by 8% polyacrylamide gel electrophoresis has been shown to stimulate the reverse transcription reaction in vitro and to be RNA primer. The stimulating activity of the primers has been shown to decrease as follows: 4S nuclear RNA of the rat liver oligo (dT)12-18 4S RNA of influenza virion. the template activity of high molecular weight genome RNA of influenza virion has been demonstrated to be negligible in vitro both in the presence of synthetic and natural primer.
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PMID:[Study of influenza virus 4S RNA as a primer in reverse transcription]. 615 54

Hybridomas were prepared by fusion of mouse myeloma cell line Sp2/0 with lymphocytes from mice immunized with avian myeloblastosis virus (AMV). The specificity of each monoclonal antibody was characterized by radioimmunoassay (RIA) using purified viral core proteins, immunoprecipitation of radioactively labeled virus (35S-methionine-labeled AMV, 125J-labeled AMV) and immunoblotting. One monoclonal antibody (IC11) which is of IgG1 subclass, and two other monoclonal antibodies (IF9 and IB8), both of IgG3 subclass, were directed against the p19 protein of AMV. The remaining eight monoclonal antibodies (most of them of IgM class) did not precipitate viral proteins under the experimental conditions used, except IIG12 hybridoma antibody which irregularly precipitated glycoprotein gp85. Since most of them (seven, including IIG12) gave positive reactions in RIA with antigenically unrelated influenza virus, these monoclonal antibodies were directed against virus components specified by chick cells (host cell antigen).
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PMID:Production and characterization of monoclonal antibodies against avian myeloblastosis virus. 631 35

This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScript reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme has demonstrated higher thermal stability and is described as suitable to make long cDNA with a complex secondary structure. The product obtained by this method can be cloned, used in later sequencing reactions or nested-PCR with the purpose of achieving a rapid diagnosis and characterization of the equine influenza virus type A. This detection assay might be a valuable tool for diagnosis and screening of field samples as well as for conducting molecular studies.
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PMID:Complete genome amplification of equine influenza virus subtype 2. 2008 82