Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0598934 (
tumor growth
)
58,965
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The present study investigated
ubiquitin specific peptidase 39
(
USP39
) gene knockdown on SMMC-7721 cells
in vitro
and
in vivo
, and the role of
USP39
in regulating the growth of hepatocellular carcinoma (HCC). Two small interfering RNAs (siRNA) were constructed, which targeted the
USP39
gene and control sequences were synthesized and inserted into a pGCSIL-GFP lentiviral vector. The full length of
USP39
cDNA was amplified by polymerase chain reaction (PCR) and cloned into pEGFP-N2, and the recombinant plasmids were transfected into cells. Knockdown efficiency and upregulation of
USP39
was determined by reverse transcription-quantitative PCR and western blotting. The impact of
USP39
on the growth of SMMC-7721 cells
in vitro
was examined using an MTT assay, colony formation, flow cytometry (FCM) and immunohistochemical staining. The impact of
USP39
on the growth of SMMC-7721 cells
in vivo
was examined by assessing tumorigenicity in nude mice. Western blotting was performed to examine the mechanism of
USP39
regulation on SMMC-7721 cell growth. Recombinant vectors containing specific and scrambled
USP39
siRNA sequences were constructed and transfected into SMMC-7721 cells.
USP39
knockdown inhibited cell proliferation and colony formation in SMMC-7721 cells, while upregulation of
USP39
promoted the growth of tumor cells. FCM indicated that
USP39
knockdown led to G2/M arrest and induced apoptosis in SMMC-7721 cells.
USP39
knockdown inhibited xenograft
tumor growth
in nude mice and led to the downregulation of the transcription factor Forkhead Box M1 (FoxM1). Gene expression of FoxM1 targets, including polo-like kinase 1, cyclin B1 and centromere protein A also decreased following
USP39
knockdown. The results suggest that knockdown of
USP39
inhibits the growth of HCC
in vitro
and
in vivo
, potentially through the induction of G2/M arrest by regulating the pre-mRNA splicing of FoxM1.
...
PMID:USP39 regulates the growth of SMMC-7721 cells via FoxM1. 2841 1
