Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0598934 (tumor growth)
 58,965 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of Lysozyme (hen egg-white lysozyme) and of its modified derivative mPEG-Lyso, (Lysozyme coupled with monomethoxypolyethylenglycol) were tested on CBA mice bearing MCa mammary carcinoma. mPEG-Lyso, given by the oral route at a dose comparable to 100 mg/kg/day of native Lysozyme, is at least as active as Lysozyme for the activation of lymphocytes obtained from different districts along the axis GALT-spleen. These effects were evidenced by measuring the in vitro response of lymphocytes of animals treated in vivo with ConA and LPS using the SRB test, and measuring the content of nucleic acids by cytofluorimetric analysis. Lymphocytes obtained from the mesenteric lymph nodes of animals treated with mPEG-Lyso, show a response to ConA and to LPS at early stages of treatment, when tumor growth reduces the response to controls. mPEG-Lyso, was also effective on lung metastasis formation. Considering that mPEG-Lyso,, compared to the native Lysozyme, completely lost its enzymatic action on Micrococcus lysodehycticus cell walls, this data suggest that the effects of lysozyme on immunity and on tumour growth are unrelated to the production of immunoactive peptidoglycans in the gut.
 ...
PMID:Antimetastatic action and lymphocyte activation by the modified lysozyme mPEG-Lyso in mice with MCa mammary carcinoma. 891 51

Lysozyme (hen egg-white lysozyme) and its derivative mPEG-lyso (lysozyme coupled with polyoxyethyleneglycol) were tested in CBA mice bearing MCa mammary carcinoma for their effects on intestinal mucosal immunity (GALT) and mesenteric lymph node lymphocytes (MLNL), after oral administration. Following a cycle of administration of 100 mg/kg/day lysozyme or 350 mg/kg/day mPEG-lyso for 9 consecutive days, GALT was analyzed by using optical histology, and mesenteric lymph node lymphocytes were studied by cytofluorimetric analysis of CD3, CD4 and CD8 antigens, and of DNA and RNA content following in vitro culture with concanavalin A. Both lysozymes significantly increase the number of lymphatic nodules on gut epithelium as determined by histological analysis of sections of small bowel. mPEG-lyso, unlike native lysozyme, gives protection from the decline of the blastogenic activity of MLNL observed at early stages of tumor growth, as shown by the increased nucleic acid content of these cells. On the same cells, both lysozyme and mPEG-lyso also seem to prevent the decline of CD4+ cells observed during tumor growth in control animals. These data confirm the effects of lysozyme on GALT and show that the new lysozyme derivative mPEG-lyso has effects on host immunity greater than those of the native molecule.
 ...
PMID:Stimulation of GALT and activation of mesenteric lymph node lymphocytes by a modified lysozyme in CBA mice with MCa mammary carcinoma. 941 23

The effects of lysozyme (hen egg-white lysozyme) on lymphocytes harvested from mice bearing MCa mammary carcinoma were tested at the daily dose of 100 mg/kg given orally for 10 consecutive days. With this experimental paradigm that consists of a significant reduction of lung metastasis formation, lysozyme was seen to be capable of stimulating the recovery of the response to ConA of mononuclear cells, reduced by tumor growth; this effect was particularly evident with splenocytes and GALT lymphocytes. The effects of lysozyme depended: (i) on the presence of plastic adherent cells, (ii) at least in in vitro experiments, on the amount of lysozyme used, being higher at the dose of 250 mug/ml of incubation mixture, and (iii) on the time of challenge with lysozyme. These effects were shown either by measuring the [H-3]-thymidine incorporation into DNA or by application of the sulphorhodamine test for protein synthesis. After in vivo treatment, lysozyme significantly modified the histological architecture of the mucosal immunity of the gut, by causing a significant reduction of the number of the lymphatic nodules placed immediately under the layer of epithelial cells of villi. At the same time no significant modification of Peyer's patches was noted. Together these data stress the role of lysozyme in the modulation of host immunity and, in particular, point out the relevance of the mucosal immunity of the gut as first target for lysozyme activity.
 ...
PMID:Lysozyme induced recovery of the response to cona on mononuclear-cells harvested from mice bearing mca mammary-carcinoma. 2156 96