Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0598934 (tumor growth)
 58,965 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The efficacy of vaccines consisting of (1) Ig idiopeptides of a murine B-lymphoblastoma, 2C3, and (2) a corresponding scFV-expressing DNA construct was determined on the basis of their ability to induce anti-tumor immune response and protection against tumor growth. Peptide-KLH complexes and a plasmid expressing single-chain variable fragment (scFv) corresponding to 2C3 Ig VH and VL chains were used separately as vaccines. Immunized BALB/c mice were bled and then challenged with live 2C3 tumor. Survival of various challenged groups was also determined. The results indicate that CDR3-H (VH3) peptides, and the plasmid DNA when given with GM-CSF, only moderately retarded tumor growth, whereas killed 2C3 tumor vaccine induced lasting protection, as shown earlier. Both peptides and scFv-plasmid induced circulating anti-Id antibodies, but no specific cytotoxic T-cell (CTL) activity was detected. However, in spite of their inability to induce CTL activity, both idiopeptides and the scFv-expressing plasmid DNA displayed epitopes recognized by an Id-specific long-term splenic CTL line (A102) obtained from mice vaccinated with killed 2C3 tumor.
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PMID:Anti-lymphoma immunity: relative efficacy of peptide and recombinant DNA vaccine. 1171 53

Microvesicles (MVs) play a pivotal role in cell-to-cell communication. Recent studies demonstrated that MVs may transfer genetic information between cells. Here, we show that MVs derived from human adult liver stem cells (HLSC) may reprogram in vitro HepG2 hepatoma and primary hepatocellular carcinoma cells by inhibiting their growth and survival. In vivo intratumor administration of MVs induced regression of ectopic tumors developed in SCID mice. We suggest that the mechanism of action is related to the delivery of microRNAs (miRNAs) from HLSC-derived MVs (MV-HLSC) to tumor cells on the basis of the following evidence: (a) the rapid, CD29-mediated internalization of MV-HLSC in HepG2 and the inhibition of tumor cell growth after MV uptake; (b) the transfer by MV-HLSC of miRNAs with potential antitumor activity that was downregulated in HepG2 cells with respect to normal hepatocytes; (c) the abrogation of the MV-HLSC antitumor effect after MV pretreatment with RNase or generation of MVs depleted of miRNAs; (d) the relevance of selected miRNAs was proven by transfecting HepG2 with miRNA mimics. The antitumor effect of MV-HLSC was also observed in tumors other than liver such as lymphoblastoma and glioblastoma. These results suggest that the delivery of selected miRNAs by MVs derived from stem cells may inhibit tumor growth and stimulate apoptosis.
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PMID:Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs. 2273 96