Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0595921 (
intraocular pressure
)
11,750
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The rabbit retina has been utilized as a model for the study of abnormal cellular proliferation on the retinal surface and into the vitreous, a process commonly initiated by trauma and generally leading to retinal detachment. This study characterizes the ability of alpha-difluoromethylornithine (alpha-DFMO), a suicide inactivator of L-
ornithine decarboxylase
(
EC 4.1.1.17
) to inactivate normal retinal
ornithine decarboxylase
(
ODC
) activity in the crude supernatant fraction after incubation with different concentrations of alpha-DFMO and at various times after intraocular administration. Partial inactivation of
ODC
activity occurred following preincubation of crude retinal supernatant fraction with 10(-5) M alpha-DFMO (N = 3; 34 +/- 6.9% of control), whereas preincubation with 10(-8) M alpha-DFMO did not alter
ODC
activity significantly (N = 3; 94 +/- 2% of control). Different concentrations of alpha-DFMO administered intraocularly inactivated retinal
ODC
activity to varying degrees with different rates of recovery. No gross toxicity occurred with ocular tissues following intravitreal administration of alpha-DFMO as determined by electrophysiologic measurements, by indirect examination of the retina, and by measurement of
intraocular pressure
. These results suggest that alpha-DFMO may be a useful tool in which to define the physiologic role of
ODC
and polyamines in intraocular cellular proliferative diseases.
...
PMID:Ornithine decarboxylase activity in rabbit retina following treatment with alpha-difluoromethylornithine. 312 Jul 37
