Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0546837 (esophageal cancer)
 8,907 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We aimed to establish the first monoclonal antibody (MAb) against the human EMILIN-5 protein (elastin microfibril interface located protein-5) and to investigate its distribution in normal human esophageal tissues and esophageal carcinomas. The bacterially expressed 6 His-EMILIN-5 fusion protein was induced and purified. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6x His-EMILIN-5 fusion proteins or purified 6x His-ZNRD1 fusion protein as a control. The EMILIN-5 protein-specific MAb was further identified by Western immunoblot analysis. The expression of EMILIN-5 was investigated in 63 cases of esophageal cancer specimens and 60 cases of normal esophageal specimens using immunohistochemical analysis. The expression of 6x His-EMILIN-5 fusion proteins was successfully induced. One MAb, H7 (IgG1), effective in detecting the recombinant and the cellular protein, was characterized. H7 bound to native EMILIN-5 protein and should be useful in studies of EMILIN-5 protein function and expression. EMILIN-5 staining was found positive in 37 cases of esophageal cancer tissues (59%) and 7 cases of normal esophageal tissues (12%). The higher-grade frequency of expression of EMILIN-5 in normal esophageal tissues was significantly lower (p < 0.01) than that in tumor tissues. We concluded that EMILIN-5 might play important roles in carcinogenesis of esophageal cancer.
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PMID:Preparation and characterization of a novel monoclonal antibody specific to human EMILIN-5 protein. 1815 87

The expression of ZNRD1 in esophageal cancer was first investigated by immunohistochemical analysis, RT-PCR and real-time PCR. The ZNRD1 antibody produced a consistently cytoplasmic staining pattern in all epithelial cells. The expression of ZNRD1 was statistically correlated with differentiation, depth of invasion, lymph node metastasis, pathological stage, lymphatic invasion, and vascular invasion. The survival rates of patients with ZNRD1-negative tumors tended to be statistically lower than those with ZNRD1-positive tumors. ZNRD1 expression was also confirmed to be down-regulated in esophageal cancer tissues compared to adjacent non-neoplastic tissues. The results showed that ZNRD1 might play an important role in esophageal carcinogenesis.
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PMID:Expression and prognostic value of ZNRD1 in esophageal squamous cell carcinoma. 1859 68