Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0546837 (esophageal cancer)
 8,907 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The objective of this study was to investigate the role of ErbB3-binding protein 1 (Ebp1) in the growth of esophageal squamous cell carcinoma (ESCC) cells and the underlying mechanism. Eca109 and KYSE150 cells were transfected with lentiviral vector carrying Ebp1 gene. The mRNA levels of Ebp1 in esophageal cancer tissues and paired adjacent normal tissues were examined by real-time PCR. The growth and viability of esophageal carcinoma cells were assessed using MTT and crystal violet assays, respectively. Clone-forming abilities of Eca109 and KYSE150 cells were analyzed by soft agar assay. Apoptotic rates of esophageal carcinoma cells were detected by flow cytometry, and expression levels of the proteins involved in apoptosis were assessed by Western blot. Tumorigenicity of Eca109 cells were detected by nude mouse transplantation tumor experiment. The results indicated that the mRNA levels of Ebp1 in esophageal cancer tissues was down-regulated compared with paired adjacent normal tissues. The growth and viability of Eca109 and KYSE150 cells were all suppressed by Ebp1 overexpression. Besides, Ebp1 overexpression induced apoptosis, increased Rb and P53 expressions, and decreased CyclinD1 expression in Eca109 and KYSE150 cells. In addition, Ebp1 overexpression inhibited the tumorigenesis of Eca109 cells in vivo. These results suggest that Ebp1 may suppress the growth of esophageal carcinoma cells in vitro and in vivo by inducing apoptosis.
 ...
PMID:[Up-regulation of ErbB3-binding protein 1 inhibits the growth of esophageal carcinoma cells]. 2800 68

Esophageal cnacer is one of the common malignant tumors with high fatality rate and morbidity, ErbB3-binding protein 1 (EBP1) is the intracellular binding protein of ErbB3. This study aims to investigate the effect of EBP1 on esophageal cancer biological behavior and the underlying mechanism. In our study, esophageal cancer tissues and adjacent non-tumor issues were collected and the relative expression of EBP1 mRNA were examined by real-time PCR. EBP1 mimics is synthesised and transfected onto Eca109 cells. The effects of EBP1 on biological behaviors of Eca109 cells were detected by colony formation assay and flow cytometry, the expression of bcl, p53 proteins were detected by western-blot. The results showed that EBP1 was down-regulation in NSCLC. The number of colony-forming units, and the tumor size and volume in vivo in the Eca109/EBP1 group was obviously lower and the apoptosis rate was significantly higher than the Eca109/NC group and Normal group (P<0.05). The expression of p53 protein in Eca109/EBP1 group were significantly lower, and the expression of bcl-2 were dramatically increased compare with Eca109/NC group and Normal group (P<0.05). Therefore, we concluded that that E-cad may inhibit cell proliferation of esophageal cancer in vitro and in vivo by inducing apoptosis. EBP1 may play a anti-oncogene role in esophageal cancer.
 ...
PMID:ErbB3-binding protein 1 inhibits tumor proliferation in esophageal cancer by targeting bcl-2 and p53 gene. 3196 54