Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0519030 (
Klebsiella
)
21,988
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The Cht gene encoding cyanide hydratase (
CHT
, EC 4.2.1.66), which detoxifies HCN and is thought to be important in fungal infection of cyanogenic plants, has been cloned from the phytopathogenic fungus Gloeocercospora sorghi. The gene was isolated by screening an expression library of G. sorghi using a
CHT
-specific antibody and using one of the positive cDNA clones as a probe in Southern hybridization to identify a 3.1 kb PstI genomic fragment. This PstI fragment expressed
CHT
activity when transformed into Aspergillus nidulans, a fungus that normally lacks
CHT
activity. Sequence analysis identified a single open reading frame of 1,107 base pairs which encodes a polypeptide of 40,904 daltons. The deduced amino acid sequence of
CHT
shares 36.5% identity to a nitrilase from the bacterium
Klebsiella
pneumoniae subsp. ozaenae.
...
PMID:Cloning and properties of a cyanide hydratase gene from the phytopathogenic fungus Gloeocercospora sorghi. 138 13
