Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0519030 (Klebsiella)
 21,988 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Epstein-Barr virus transformed lymphoblastoid cell lines from HLA-B27 positive individuals with ankylosing spondylitis (B27+AS+) release, into the culture medium, a factor capable of specifically modifying the HLA-B27 positive lymphocytes of normal individuals (B27+AS-); this modification results in a phenotypic change similar to that seen on B27+AS+ lymphocytes. This lymphoblastoid cell line derived factor appears to be physically and functionally similar to a factor present in the culture filtrate of certain Klebsiella isolates. Biogel P-100 chromatography of the material released from the cell line indicated a mol.wt of 25,000-30,000, similar to that of the Klebsiella derived factor. Chromatofocusing on a PBE 94 column revealed that cell line derived factor had an isoelectric point of 5.5 (cf. pI 5.4 for the Klebsiella derived factor). Immunoadsorption experiments suggest that the factor from the B27+AS+ cell line shares antigenic determinants with a cell surface component present on certain Klebsiella isolates. These results will form the basis for future studies on the nature of the interaction between HLA-B27 and certain enteric organisms and their products. A better understanding of this association should elucidate some of the early events in the pathogenesis of the seronegative arthropathies.
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PMID:A factor shed by lymphoblastoid cell lines of HLA-B27 positive patients with ankylosing spondylitis, specifically modifies the cells of HLA-B27 positive normal individuals. 619 93

We have evaluated claims that impaired peripheral blood lymphocyte (PBL) transformation can occur with Klebsiella spp. in patients with ankylosing spondylitis (AS). PBL of four AS patients were cultured in vitro with autogenous faecal klebsiella, as were the PBL of age (+/- 3 years) and sex-matched pairs of 15-20 AS and normal controls cultured with heterogeneous AS-derived klebsiella and control bacterial isolates. Three of four AS patients responded to their own isolates, and no significant differences were found between the matched pairs in response to heterogeneous klebsiella isolates, including K21. Our studies did not show impaired PBL transformation with klebsiella in AS and therefore do not support claims of antigenic cross-reactivity between klebsiella and HLA-B27.
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PMID:Absence of impaired lymphocyte transformation to Klebsiella spp. in ankylosing spondylitis. 633 81

Faecal samples obtained from patients with acute anterior uveitis (AAU) and healthy controls unconnected with hospitals were examined for the presence of Gram-negative micro-organisms reported to be associated with AAU. There was an increased recovery of Klebsiella spp. from AAU patients seen in the first and in the second week after onset of symptoms when compared with patients presenting three to four weeks after onset of symptoms (p less than 0.025 and p less than 0.05 respectively) or with healthy control subjects (p less than 0.005 and p less than 0.05 respectively). By the third to fourth week there was no difference in klebsiella recovery between AAU patients and controls. The increased faecal recovery of klebsiella in the first two weeks was predominantly in HLA-B27 positive patients or patients possessing antigens which cross-react with B27, namely, HLA-B7 CREG. The patients with faecal cultures positive for klebsiella had a higher mean ESR than patients with negative faecal cultures (p less than 0.05). Although there was an increase in recovery of faecal klebsiella from AAU patients with spondylarthritis when compared with AAU patients without arthritis, the difference was not statistically significant. These results suggest that some klebsiella micro-organisms may play a role in the aetiopathogenesis of AAU associated with HLA-B27 or HLA-B7 CREG.
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PMID:A search for gram-negative enteric micro-organisms in acute anterior uveitis: association of klebsiella with recent onset of disease, HLA-B27, and B7 CREG. 633 44

The possibility that plasmid genes, carried by enteric organisms previously indirectly implicated as disease agents, play a role in the pathogenesis of Ankylosing Spondylitis (AS) was explored. A particular Klebsiella isolate (K21) previously found to cross-react with cells from HLA-B27 positive (B27+) patients with AS, but not with cells from normal individuals, was found to contain a plasmid(s). This coded for the organism's ability to produce a factor which could modify B27+ normal cells (AS-) rendering them lysable by the anti-Klebsiella serum. Curing of this isolate resulted in the loss of the plasmid concerned and a loss of ability of its culture filtrate to modify B27+ lymphocytes of clinically healthy subjects. When plasmids from K21 were transferred to a plasmid free laboratory strain, E. coli JP995, the recipient strain acquired the ability to elaborate modifying factor. These data suggest that plasmids, harboured by some enteric bacteria, and their products, may be implicated in modifying cells bearing certain Major Histocompatibility Complex genes, and that such modification may be an important factor in the pathogenesis of a number of diseases including the seronegative arthropathies.
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PMID:Is a Klebsiella plasmid involved in the aetiology of Ankylosing Spondylitis in HLA-B27-positive individuals? 634 14

One-hundred eighty-five clinical isolates of Salmonella sp., Shigella sp., Escherichia coli, and Campylobacter sp. were tested for their ability to absorb the lymphocytotoxic activity of an antiserum (anti-Klebsiella sp. K43) directed against a specific HLA-B27-associated cell surface determinant on the lymphocytes of patients with ankylosing spondylitis (AS). Seven of these isolates (three Salmonella sp., two Shigella sp., one E. coli, and one Campylobacter sp.) were found to cross-react with the B27-positive cells of AS patients (B27+ AS+); an E. coli organism isolated from the rectal swab of an HLA-B27-negative clinically normal individual also cross-reacted with B27+ AS+ cells. These cross-reactive enteric organisms elaborate a factor (modifying factor) which specifically modifies the B27-positive lymphocytes of normal individuals; this factor is structurally and antigenically related to a functionally similar factor secreted by certain isolates of Klebsiella sp. These data suggest that certain enteric organisms share a common determinant which cross-reacts with B27+ AS+ cells. It is suggested that this cross-reactivity is somehow related to an early event in the pathogenesis of AS and possibly of other seronegative arthropathies.
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PMID:Possible role of enteric organisms in the pathogenesis of ankylosing spondylitis and other seronegative arthropathies. 635 Jan 89

Lymphocyte transformation and leucocyte migration inhibition was studied in Klebsiella-sensitized patients, ankylosing spondylitis patients and healthy controls. The results show that mononuclear cells from ankylosing spondylitis patients respond more vigorously to Klebsiella, one of the 'trigger' bacteria, than do mononuclear cells from healthy persons, either with or without HLA-B27.
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PMID:Klebsiella-induced LIF response in Klebsiella infection and ankylosing spondylitis. 636 Feb 92

The association between HLA-B27 and ankylosing spondylitis could be explained by a theory of crossed tolerance, which proposes that Gram negative bacteria have antigens similar to HLA-B27. Experimental studies with human sera with anti-HLA specificity and rabbit sera with anti-Klebsiella specificity demonstrate a partial crossed reactivity between HLA-B27 and Klebsiella. Clinical studies have shown that Klebsiella can be isolated from patients with ankylosing spondylitis during relapses or active phases of the disease. The theory of crossed tolerance proposes that ankylosing spondylitis is a reactive arthritis which develops after infection with Gram negative bacteria. The agent directly involved in causing the disease is an anti-bacterial antibody which binds to self antigens with partial crossed reactivity, such as HLA-B27, and the inflammation develops as a result of triggering of the complement cascade by the antigen-antibody complex.
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PMID:[Ankylosing spondylarthritis, HLA B27 and the theory of crossed tolerance]. 636 15

Samples of the faeces of 153 consecutive patients presenting with acute anterior uveitis (AAU), and of 47 controls were examined for the presence of Klebsiella pneumoniae. No increase in the carriage rate of klebsiella was found in the AAU patients as compared with the controls. Furthermore no increase was found in any group of patients whether subdivided by HLA-B27 status, sex, or presence of ankylosing spondylitis (AS). No difference was found between patients having their first attack of AAU and those with recurrences.
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PMID:Klebsiella and acute anterior uveitis. 639 34

The possibility that a plasmid carried by Klebsiella pneumoniae plays a role in the pathogenesis of ankylosing spondylitis was explored. K. pneumoniae K21 contains a congruent to 25-kb plasmid, but this plasmid is not present in lymphocyte DNAs of ankylosing spondylitis HLA-B27 patients, as demonstrated by molecular hybridization experiments.
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PMID:Klebsiella plasmid K21 is not involved in the aetiology of ankylosing spondylitis. 640 Nov 43

The association of HLA-B27 with ankylosing spondylitis (AS) can be explained by a cross-tolerance hypothesis, which suggests that Gram-negative bacteria, such as Klebsiella, possess antigens which resemble HLA-B27. Experimental studies with human tissue-typing sera and rabbit anti-Klebsiella sera would appear to be compatible with this hypothesis. Clinical studies indicate that faecal Klebsiella can be isolated more readily from AS patients during active phases of the disease, when this is defined either clinically, with or without uveitis, or biochemically by elevation in ESR and C-reactive protein levels. The cross-tolerance hypothesis proposes that ankylosing spondylitis is a reactive arthritis following infection by Gram-negative bacteria and tissue damage is produced by antibacterial antibody binding to cross-reacting self-antigens.
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PMID:The cross-tolerance hypothesis, HLA-B27 and ankylosing spondylitis. 641 59


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