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Query: UMLS:C0519030 (Klebsiella)
 21,988 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Capsular and somatic antigens were determined in 100 Kl. oxytoca strains isolated from patients with the respiratory tract inflammations. K antigens were assigned by the capsula swelling test using 77 specific anti-capsular sera. Most frequent were: K14, K2, K55, K8 and K16 antigens. Positive reaction was noted with 64 strains in 2 or more sera. Somatic antigens of Klebsiella oxytoca bacilli were tested by the test tube agglutination reaction. Of 63 strains tested with anti-01 Kl. pneumoniae and Kl. oxytoca sera, all reacted positively in anti-0 Kl. oxytoca serum and 77% strains in anti-01 Kl. pneumoniae serum. Of 29 strains agglutinating in anti-03 sera, 65.5% agglutinated with anti-0 Kl. oxytoca serum and 76% with anti-03 Kl. pneumoniae. The results have revealed that Kl. oxytoca strains investigated have more complicated capsular antigens and different frequency of their occurrence. The most commonly encountered somatic antigen is antigen 01, next in turn is antigen 03, these antigens proved nonidentical with their 01 and 03 counterparts in Kl. pneumoniae strains.
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PMID:Capsular and somatic antigens of Klebsiella bacilli. 171 35

An investigation, including environmental sampling, was undertaken after four leukaemic patients on the same hospital ward developed serious infections with Klebsiella aerogenes, capsular type K14. The source of this organism, common to all four patients, was found to be a food blender used for preparing milk-based drinks on the ward.
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PMID:An outbreak of serious Klebsiella infections related to food blenders. 288 28

Carumonam, a new monobactam, was found to have an anti-microbial spectrum similar to aztreonam. Its spectrum includes Enterobacteriaceae, Haemophilus influenzae, pathogenic Neisseria species, Pseudomonas aeruginosa, and some streptococci. Staphylococcus species, enterococci, and many other nonenteric gram-negative bacilli were not inhibited. Enterobacteriaceae resistant to cefoperazone (minimum inhibitory concentrations [MICs] greater than or equal to 32 mg/L) were more likely inhibited by carumonam (52% at less than or equal to 8.0 mg/L) than aztreonam (39%) or ceftazidime (35%). Dilution test methods on agar or in Mueller-Hinton broth produced similar results. Carumonam minimum bactericidal concentrations were usually the same or one dilution above the MIC. Carumonam and aztreonam were very stable to most chromosomal (P99, K1, K14) and plasmid-mediated beta-lactamases (TEM, OXA, PSE). The Klebsiella oxytoca enzymes hydrolyzed aztreonam at rates greater than or equal to fivefold higher than carumonam but at a rate less than 1% that of cephaloridine. The aztreonam MICs for these Klebsiella stains were greater than or equal to 32 mg/L, but the hydrolysis rates do not fully explain the high-grade resistance to aztreonam. In vitro susceptibility tests with 30-micrograms carumonam disks were found to be very predictive. Similar regression statistics were observed for aztreonam and cefotaxime. Recommendations for carumonam susceptibility testing are susceptible greater than or equal to 21 mm (less than or equal to 8.0 mg/L) and resistant less than or equal to 14 mm (greater than or equal to 32 mg/L). Cross-resistance analysis favors the independent testing of carumonam or aztreonam against gram-negative species other than Enterobacteriaceae and P. aeruginosa.
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PMID:The anti-microbial activity, beta-lactamase stability, and disk diffusion susceptibility testing of carumonam (RO 17-2301, AMA-1080), a new monobactam. 309 30

Klebsiella K14 capsular polysaccharide was degraded by a bacteriophage-borne enzyme to afford oligosaccharides A-C which were studied by one- and two-dimensional n.m.r. spectroscopy. A and B were the repeating-unit hexasaccharide and pyruvylated hexasaccharide, respectively, while C was a dodecasaccharide. Each oligomer was terminated by a reducing mannose and a non-reducing 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid residue, indicating that the phage enzyme had cleaved the beta-D-Manp-(1----4)-beta-D-GlcpA linkages in the polysaccharide by a lyase, rather than the more common glycosidase, activity found with other Klebsiella bacteriophages. In this respect, the depolymerisation resembles those reported for the capsular polysaccharides of Klebsiella K5 and K64
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PMID:Preparation of branched hexasaccharides by the action of a viral lyase on Klebsiella K14 polysaccharide. 324 1

The structure of the capsular polysaccharide isolated from Klebsiella serotype K14 has been investigated employing a combination of chemical and spectroscopic methods. The repeating structure is shown to be of the "4 + 1 + 1" type, and it carries a 1-carboxyethylidene acetal substituent at positions 4 and 6 of a terminal glucose residue. The polysaccharide is one of a group of only three Klebsiella polysaccharides that have been found to contain a galactofuranose residue in the repeating unit. The repeating unit has the following structure: (Formula: see text).
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PMID:A structural investigation of the capsular polysaccharide of Klebsiella K14. 405

The present study reports on the results of capsular serotyping of Klebsiella strains isolated between 1971 and 1972, carried out in view of the subdivision of the genus. Serologic typing was performed with K1--K80 sera on 156 Klebsiella strains, isolated from in-hospital cases of extraenteral klebsiella infections. The 140 (90%) strains typed were listed in 34 capsular serotypes. Another 16 strains (10%) could not be typed because of their insufficient capsular coating. The following 13 serotypes were predominant: K4 (13.57%), K2 (7.85%), K16 (6.42%), K18 (6.42%), K27 (5%), K20 (4.28%), K24 (4.28%), K55 (4.28%), K68 (4.28%), K48 (3.55%), K15 (2.85%), K17 (2.85%), representing 70.71%. In the feces samples from the healthy controls serotypes K7, K14, K30, K1, K2, K24, K27, K47 etc. may be considered omnipresent. It has been demonstrated that in periods of minimal epidemiological survey, these serotypes from healthy carriers may initiate nosocomial infections. The present findings emphasize the accessibility and value of serologic typing of klebsiellas according to clinical and epidemiologic indications. The serologic type is a marker that can be included on the programme of systematic typing of in-hospital strains.
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PMID:[Evaluation of the effectiveness of serological typing in subdifferentiation of strains of Klebsiella of extraenteral origin]. 699 88

The method for serological typing of Klebsiella pneumoniae making use of Russian commercial K-sera manufactured by the Ilya Metchnikoff firm has been used to characterize 85 strains isolated from newborns at an obstetrical hospital and department of newborn diseases and from children with acute enteric infections hospitalized at the hospital for infectious diseases. The authors emphasize that their methods of serotyping are to be accurately performed, specifically, the selection of capsular forms and identification of serovars in strains which can be agglutinated by several sera. Serovars were identified by the proposed serotyping method in 89.4% of the studied strains. A wide spectrum of K-serovars typical of this or that hospital has been defined for each institution. K. pneumoniae K2 predominated in the obstetrical hospital, K. pneumoniae K24 and K25 prevailed in department for newborn diseases, and the K14 variant in the infectious diseases hospital.
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PMID:[Methodological aspects of Klebsiella pneumoniae serotyping and its use for characterization of clinical isolates]. 937 9

Interaction of Klebsiella K14 capsular polysaccharide with cationic dyes pinacyanol chloride, acridine orange and phenosafranin has been studied by spectrophotometric and spectrofluorometric techniques. The polymer containing both glucuronic acid and pyruvic acid in its repeating unit behaved as a unique polyelectrolyte. It induced blue shift of the absorption band of pinacyanol chloride indicating strong metachromasy. Stoichiometry of the polyanion and the dye cations in the polymer-dye compound (1:2) indicated that both glucuronic acid and pyruvic acid acted as potential anionic sites for interaction with the cationic dye molecules. The stoichiometry of anionic site (of polyanion): cationic site (of dye) in the polymer dye compound was calculated as 1:1. Interaction of the polymer with acridine orange and phenosafranin dyes studied by fluorescence measurements demonstrated Stern-Volmer type of quenching. Equivalent weight of the polymer was determined by spectrophotometric and spectrofluorometric titrations. From the present studies chromotropic property of the polymer was established.
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PMID:Spectrophotometric and spectrofluorometric studies on interaction of cationic dyes with bacterial capsular polysaccharide. 985 5

A total of 92 clinical isolates of Klebsiella planticola from man was examined with respect to the production of haemagglutinins and siderophores, serum resistance and distribution of capsular types. For comparison, a group of 207 clinical isolates of K. pneumoniae was also studied. The percentages of K. planticola strains able to express mannose-sensitive haemagglutination, indicating type 1 fimbriae (83%) and mannose-resistant and Klebsiella-like agglutination, indicating type 3 fimbriae (69%), as well as to produce the siderophores enterobactin (100%) and aerobactin (2.2%) were almost identical to those of the K. pneumoniae strains. Similarly, the proportion of serum-resistant strains (30%) was comparable to that of K. pneumoniae (25%). The capsule types most often detected in K. planticola were K14 (13%), K2 (9%) and K70 (9%). The incidence of K2, which is the predominant capsular type in K. pneumoniae, was similar in both species. These findings show that K. planticola, which is being detected with increasing frequency in clinical specimens from man, has the ability to express similar putative virulence factors to K. pneumoniae, suggesting that they may have similar pathogenicity.
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PMID:Expression of putative virulence factors by clinical isolates of Klebsiella planticola. 1067 May 61

Klebsiella pneumoniae is an enteric pathogen causing community-acquired and hospital-acquired infections in humans. Epidemiological studies have revealed significant diversity in capsular polysaccharide (CPS) type and clinical manifestation of K. pneumoniae infection in different geographical areas of the world. We have sequenced the capsular polysaccharide synthesis (cps) region of seven clinical isolates and compared the sequences with the publicly available cps sequence data of five strains: NTUH-K2044 (K1 serotype), Chedid (K2 serotype), MGH78578 (K52 serotype), A1142 (K57 serotype) and A1517. Among all strains, six genes at the 5' end of the cps clusters that encode proteins for CPS transportation and processing at the bacterial surface are highly similar to each other. The central region of the cps gene clusters, which encodes proteins for polymerization and assembly of the CPS subunits, is highly divergent. Based on the collected sequence, we found that either the wbaP gene or the wcaJ gene exists in a given K. pneumoniae strain, suggesting that there is a major difference in the CPS biosynthesis pathway and that the K. pneumoniae strains can be classified into at least two distinct groups. All isolates contain gnd, encoding gluconate-6-phosphate dehydrogenase, at the 3' end of the cps gene clusters. The rmlBADC genes were found in CPS K9-positive, K14-positive and K52-positive strains, while manC and manB were found in K1, K2, K5, K14, K62 and two undefined strains. Our data indicate that, while overall genomic organization is similar between different pathogenic K. pneumoniae strains, the genetic variation of the sugar moiety and polysaccharide linkage generate the diversity in CPS molecules that could help evade host immune attack.
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PMID:Genetic diversity of capsular polysaccharide biosynthesis in Klebsiella pneumoniae clinical isolates. 1974 90


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