Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0519030 (Klebsiella)
 21,988 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Various uniform salt forms of Klebsiella O3 lipopolysaccharide (KO3 LPS) isolated from culture supernatant were prepared as follows. Basic materials present in KO3 LPS were rigorously removed by electrodialysis and the electrodialyzed KO3 LPS was neutralized with NaOH, KOH, NH4OH, Ca(OH)2, tris(hydroxymethyl)aminomethane, or triethylamine. The ultrastructure of the uniform salt forms of KO3 LPS was examined using preparations stained with uranyl acetate. The sodium, potassium, ammonium, and trisaminomethane salt forms were structurally very similar to the natural form of KO3 LPS which consisted of a mixture of flat ribbon-like structures (average width of 16 nm and average thickness of 7 nm) and spheres with various diameters, both covered with fine hairy structures. When KO3 LPS was converted to the triethylamine salt form, the ribbon-like structures were disrupted into very small granules (7-9 nm X 9-15 nm). The calcium salt form consisted of particles and rods of various sizes and ribbon-like structures which were markedly extended (maximum width of 50 nm) and presented irregular shapes. When converted to the calcium salt form, the ribbon-like structures were extended and eventually divided into particles and rods. For reasons still unknown, the sodium salt of KO3 LPS was mostly stained positively with uranyl acetate in contrast to the natural form and the other uniform salt forms which were always negatively stained. In the positively stained preparation of the sodium salt form, it was clearly shown that the ribbon-like structures consisted of a bilayer.
 ...
PMID:Ultrastructure of Klebsiella O3 lipopolysaccharide isolated from culture supernatant: structure of various uniform salt forms. 647 35

The study of the vaccinating power of ribosomal vaccines against Klebsiella pneumoniae led us to define the chemical nature which supports this protective activity. We tried to separate this support and the ribosomes by sucrose gradient ultracentrifugation. We isolated high protective membrane vesicles by this technique applied to salt-washed ribosomal preparations. When the ribosomal preparations were exposed to SDS, the protective activity was conserved all along the gradient, with no correlation with the ribosome concentration. The addition of bovine serum albumin to the ribosomal preparation focused the protective activity on the ribosomal peak. No correlation was observed between the response to capsular polysaccharide and the vaccinating power of the fractions.
 ...
PMID:An attempt to localize the vaccinating power of Klebsiella pneumoniae ribosomal preparations using saccharose-gradient ultracentrifugation. 676 58

The destruction of amoxycillin by beta-lactamase action represents an important mechanism of bacterial resistance to the drug. Data is presented to illustrate that clavulanic acid used in the form of its potassium salt inhibits the amoxycillin destroying action of many different types of beta-lactamase for example: the staphylococcal enzyme, the clinically important plasmid mediated enzymes of the TEM, SHV, OXA and PSE types and the chromosomally controlled enzymes produced by Proteus mirabilis and Klebsiella pneumoniae. The mechanisms by which clavulanic acid inhibits beta-lactamases and potentiates the antibacterial action of amoxycillin are discussed.
 ...
PMID:Biochemistry and action of clavulanic acid. 676 60

The two pathogenic species Streptococcus pneumoniae and Klebsiella pneumoniae were used to analyze the immunogenic role of proteins in ribosomal preparations. The protective activity of ribosomes prepared from either strain and further purified by washing with high-salt concentrations, followed or not by sucrose gradient separation of the particles, was identical to that of crude unwashed ribosomes. Similarly, no substantial alteration of the level of protection was observed after treatment with the antibiotic puromycin. Therefore, the immunizing efficacy of ribosomes does not appear to be due either to the nonribosomal proteins adsorbed at the surface of organelles or to the growing polypeptide chain. It seems rather to be attributable to the structural ribosomal proteins themselves, which were indeed shown to induce alone a significant level of protection.
 ...
PMID:On the immunogenicity of ribosomes and ribosomal proteins isolated from Klebsiella pneumoniae and Streptococcus pneumoniae. 701 78

Dinitrogen-fixing (acetylene-reducing) bacteria may be readily isolated from soils but extensive biochemical or immunobiological testing, or both, are required to identify them absolutely. A computer-assisted scheme for identification of nine genera of dinitrogen-fixing bacteria was developed and tested. The computer program is based on interpretation of the 70 biochemical tests of the API 20E and 50E, supplemented with tests for acetylene reduction, nitrate and nitrite reduction, catalase, oxidase, motility, and growth on MacConkey's bile salt medium. Dinitrogen-fixing Enterobacteriaceae (Klebsiella pneumoniae, Enterobacter cloacae, and Erwinia herbicola) were accurately identified using the data base in the API analytical profile index. Nonenteric dinitrogen-fixing bacteria (Azotobacter spp., Azospirillum spp., Derxia sp., Rhodospirillum sp., Clostridium sp., and Bacillus spp.) were subjected to these tests to form a new data base for these bacteria. The API tests agreed with standard biochemical tests commonly used to identify these bacteria, were reproducible with time, and were sufficiently unique to permit accurate identification of each species. The use of the API 20E and 50E tests plus the additional seven tests with these known data bases permitted rapid and precise identification of acetylene reducing bacteria from various agricultural ecosystems.
 ...
PMID:Dinitrogen-fixing bacteria: computer-assisted identification of soil isolates. 721 18

The ability to form gas in lactose bile-salt broth at 44.5 degrees C (the "faecal coliform" or FC test), growth in nutrient broth at 10 degrees C, indole production and pectin liquefaction were studied in 480 strains of Klebsiella isolated from human and animal infections, from various sites in the hospital environment and hospital food, and from river water and flowers. A positive FC response was correlated inversely with the ability to grow at 10 degrees C. Most strains of human and animal clinical origin were FC positive, whereas strains from water and flowers were mainly FC negative. The frequency of a positive FC response in strains from the hospital environment fell between these two extremes. The production of indole and liquefaction of pectin by klebsiellas was correlated directly with the ability to grow at 10 degrees C and a negative FC response. Nearly all of the strains could be allocated to one of four groups on the basis of these tests. The capsular serotype, bacteriocine-inhibition patterns and antibiotic sensitivities of the strains were examined. No correlation was evident between the first two properties and klebsiellas from any particular source. Strains of clinical origin were more often resistant to five or more antibiotics than were strains from the hospital environment, which in turn showed a greater frequency of antibiotic resistance than did strains from river water and flowers.
 ...
PMID:A comparison of the properties of Klebsiella strains isolated from different sources. 743 73

An unusual Klebsiella strain was isolated from a deep periodontal pocket of a diabetic patient. According to its biochemical reactions the new strain differed from Klebsiella pneumoniae and other described biotypes. In addition, the new isolate was very salt tolerant; in the presence of 7.5% sodium chloride the bacterium changed into a spirillum-like form, with highly pleomorphic filaments, and reverted immediately to the short rod form at lower sodium chloride concentrations or in the absence of sodium chloride. Optimum growth was observed in the presence of 2% sodium chloride. A wide range of carbohydrates was fermented with a large amount of gas production. It appears that the adaptation of the isolated Klebsiella strain to sodium chloride allows it to enter the ecological niche of periodontal lesions.
 ...
PMID:Isolation of a salt tolerant pleomorphic Klebsiella strain from a case of diabetic periodontitis. 743 88

Cultures of Klebsiella pneumoniae fermenting sodium alginate produce an extracellular guluronate-specific alginate lyase. This enzyme production was studied in stirred-tank fermentors. Different alginate substrates gave moderate differences in growth and enzyme yield. Alginates with low guluronic content gave reduced biomass but favored enzyme production. Low molecular weight (down to DPn approximately 270) also favored enzyme production. Excessive depolymerization of substrates occurred during heat sterilization of culture media. The enzyme was characterized by its specificity and sensitivity to pH, salt, and calcium. Improved yields of viable protoplasts were documented for Laminaria digitata (Huds.) Lamour.
 ...
PMID:Production and characterization of guluronate lyase from Klebsiella pneumoniae for applications in seaweed biotechnology. 776 7

The activity of ceftriaxone plus metronidazole against pathogens usually involved in intra-abdominal infections was studied. Metronidazole 1 g and ceftriaxone 1 g (as the sodium salt) were simultaneously administered i.v. over 30 minutes every 24 hours to 12 healthy volunteers for three doses. Serum samples were collected at baseline, just before the last dose, and 12, 16, 20, 22, and 24 hours after the start of infusion of the last dose. Serum bactericidal titers (SBTs) were performed in duplicate for each serum sample from 12 hours on. Serum ceftriaxone and metronidazole concentrations were determined by high-performance liquid chromatography, and the elimination rate and half-life were calculated for each antimicrobial in each volunteer. The minimum inhibitory concentrations (MICs) of each antibiotic for two strains each of Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, and Bacteroides fragilis were determined by microdilution. Eleven volunteers completed the study. Ceftriaxone and metronidazole maintained SBTs of at least 1:4. Serum ceftriaxone concentrations remained above the MICs for E. coli, P. mirabilis, and K. pneumoniae, and serum metronidazole concentrations remained above the MIC for B. fragilis throughout the study. Ceftriaxone combined with metronidazole resulted in intense and prolonged activity against E. coli, P. mirabilis, K. pneumoniae, and B. fragilis.
 ...
PMID:Serum bactericidal activity of ceftriaxone plus metronidazole against common intra-abdominal pathogens. 794 6

Assays were performed with a Malthus AT Microbiological Analyzer to define an analytical procedure to estimate Escherichia coli counts in live bivalve shellfish by conductance measurement. The growth conditions used (Malthus Coliform Broth at 44 degrees C) were selective for E. coli, and interference was noted only when Klebsiella pneumoniae were at least 100 times as numerous as E. coli. Different sample preparation procedures and seeding conditions were tested to obtain good quality conductance curves. The best results were observed when: (a) meat and shell liquor were diluted 1:3 with tryptone salt water and homogenized in a Waring blender for 1 min at 15,000 rev min-1; and (b) the inoculum was taken from the liquid phase of the homogenate 20 min after blending and mixed immediately with the culture medium. Detection parameter threshold values were adjusted (first difference 1.5 microS for the baseline and 3.5 microS for detection, second difference 0.2 microS) to improve detection time reliability. The repeatability of conductance measurements was very good (S.D. as % response mean ranged from 1.9 to 3.3) with the protocol used.
 ...
PMID:Analytical procedure for use of conductance measurement to estimate Escherichia coli in shellfish. 798 55


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>