Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0519030 (Klebsiella)
 21,988 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Fifteen cases of chronic lymphadenitis with massive hemophagocytic sinus histiocytosis were analyzed. Fourteen patients were whites, 13 were Europeans, and 11 were males; 10 patients were under 10, 4 were over 20, and 2 over 60 years old. The oldest patient died; all other either healed without therapy or are in excellent condition. We studied the cytologic features of lymph node imprints. A 5-year-old girl was examined more thoroughly. High antibody titers to Klebsiella antigens were found repeatedly. The patient also had a constant lymphocytopenia. Phytohemagglutinin-induced blast transformation and lymphotoxin production were within normal limits. No serum Epstein-Barr virus-antibodies could be detected.
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PMID:Sinus histiocytosis with massive lymphadenopathy: fifteen new cases. 125 7

Sinus histiocytosis with massive lymphadenopathy (SHML) is a newly recognized disorder. The etiology of this disease is unknown. An exaggerated response to an offending agent such as the Epstein-Barr virus or Klebsiella bacteria has been postulated. Its course is usually benign. Cervical adenopathy is seen in 97% of the patients, while 30% of patients have nodal involvement in other sites, and 30% have extranodal involvement. There is a 7% mortality rate that occurs primarily in patients with immunologic defects. Corticosteroids ameliorate the constitutional symptoms, but cyclophosphamide appears to have the most beneficial effect. This article presents the case of a patient with SHML who demonstrated elevated Epstein-Barr virus titers.
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PMID:Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease) in a patient with elevated Epstein-Barr virus titers. 166 27

A number of human monoclonal antibodies (HmAb) recognizing type-specific determinants expressed by the lipopolysaccharide (LPS) of Pseudomonas aeruginosa and by the capsular polysaccharide (CPS) of Klebsiella were generated for potential treatment of nosocomial infections. The goal is to administer these type-specific HmAb prophylactically as a "cocktail" providing broad coverage. Lymphoblastoid cell lines (LCL) secreting HmAb recognizing P. aeruginosa LPS, toxin A or Klebsiella CPS were obtained by Epstein Barr Virus (EBV) transformation of peripheral blood lymphocytes (PBL) from donors immunized with either a polyvalent Klebsiella CPS or P. aeruginosa O-polysaccharide-toxin A conjugate vaccine. LCL secreting antibodies of the desired specificities were fused to a heteromyeloma cell line. Stable clones were selected by limiting dilution. Hybridomas secreting IgM HmAb which recognized P. aeruginosa Habs serotype 3 and 4 and all 7 Fisher immunotypes were isolated. All were able to prevent fatal experimental P. aeruginosa sepsis in mice when passively transferred. In addition, 4 lines secreting IgG HmAb which neutralize the cytotoxic activity of toxin A were characterized. IgM and IgA secreting hybridoma cells with specificity for Klebsiella CPS of 22 different serotypes were also isolated. Preliminary studies indicate that these HmAb are opsonic.
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PMID:Human monoclonal antibodies to Pseudomonas aeruginosa type-specific lipopolysaccharides, toxin A and Klebsiella capsular polysaccharides. 169 65

Presenting a panel of human hybridomas secreting serospecific antibodies which confer a high degree of protection against fatal infection with Pseudomonas aeruginosa, we report an efficient approach for a systematic generation of antigen specific human monoclonal antibodies with biological activity. This approach is based on active immunization and antigen specific panning. Individuals were immunized with polysaccharides isolated from LPS of Pseudomonas aeruginosa conjugated to toxin A. Specific B cells were isolated and enriched by panning of blood samples taken at the time point with the highest frequency of fuseable cells. These cells were then transformed with Epstein-Barr virus. Arising lymphoblastoid cell lines were screened for the secretion of anti-LPS antibodies by enzyme-linked immunosorbent assay and fused to a murine-human heteromyeloma cell line. Hybridomas were selected for high levels of antibody secretion and binding to intact bacteria as determined by an immunofluorescence microscopy assay. The observation that protective capacity of an antibody was associated with its ability to bind to LPS determinants accessible on the bacterial cell surface allowed for an effective screening for therapeutically interesting human monoclonal antibodies. Out of four immunized individuals, 15 lymphoblastoid cell lines with anti-LPS activity could be isolated, and 8 hybridomas, which cover the majority of the common Pseudomonas aeruginosa serotypes, were characterized further. The generation of monoclonal anti-Pseudomonas aeruginosa toxin A, anti-Klebsiella capsular polysaccharides, and anti-Escherichia coli LPS antibodies suggests that the success of this approach is not limited to the generation of human monoclonal antibodies of a particular specificity or to the use of antigens of a particular character.
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PMID:Systematic generation of antigen specific human monoclonal antibodies with therapeutical activities using active immunization. 212 20

Cord blood B cells were immortalized in vitro with Epstein-Barr virus (EBV). Supernatants containing greater than 500 ng/ml IgM from clones/lines were tested for expression of anti-DNA-associated 16/6 and PR4 idiotypes (id) by ELISA. Four of 70 lines, but no clones, were positive for 16/6 id and none expressed the PR4 id. The presence of 16/6 id on four cell lines was associated with specificity for ssDNA, cardiolipin and Fc of IgG. No association was seen with binding to the K30 polysaccharide of Klebsiella. One clone binding this antigen also had anti-ssDNA, anti-Fc and anti-cardiolipin activity but did not express 16/6 id. Our data support the germ-line nature of the 16/6 id and are consistent with the notion that IgM autoantibody-producing B cells use VH genes which are part of the normal B cell repertoire.
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PMID:The antibody repertoire of early human B cells. II: Expression of anti-DNA-related idiotypes. 215 85

Immunosufficiency can be evaluated by Ig secretion subsequent to mitogenic stimulation of human mononuclear cells (MNC). It seems that there are significant differences in immunoglobulin class secreted by these cells when stimulated with various polyclonal activators. The aim of the current study was to analyse these differences. MNC cells was randomly obtained from nine healthy blood donors and were activated by Epstein-Barr virus (EBV), group-A streptococcus (A-ScM), Staphylococcus aureus (SAC), Klebsiella pneumonia (Kleb-M) and pokeweed mitogen (PWM). Significantly increased levels of IgM were recorded after a 7 day incubation followed by stimulation with Kleb-M (6.2 +/- 2.9) and EBV (5.9 +/- 4.5) compared to inactivated MNC (1.6 +/- 1.4), and following 10 days incubation then stimulation by EBV (13.4 +/- 5.5) and Kleb-M (9.9 +/- 4.2) compared to unstimulated cells (2.9 +/- 1.8). Significantly greater IgG levels were achieved following incubation with EBV (3.0 +/- 4.0) and PWM (2.4 +/- 1.3) after 7 days (vs 0.6 +/- 0.4 in unstimulated cells) and by PWM (11.7 +/- 5.3) and Kleb-M (8.8 +/- 3.9, vs 2.3 +/- 2.2) after 10 days. The present data emphasize the significance of merging both mitogen selection and culture duration for acquiring information and high fidelity results of immunoglobulin secretion by polyclonal activators.
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PMID:Immunoglobulin secretion of mononuclear cells induced by various mitogens. 284 83

Peripheral blood mononuclear cells derived from 6 normal subjects were incubated with 5 polyclonal activators (pokeweed mitogen, Epstein-Barr virus, group-A Streptococcus, Staphylococcus aureus and Klebsiella pneumoniae. After 7 days of incubation, the supernatants were examined for immunoglobulin (IgG and IgM) production, the level of a common anti-DNA idiotype (16/6 Id) and autoantibody activity against ssDNA, dsDNA, poly(I), poly(dT) and cardiolipin. Significantly increased levels of the 16/6 Id were recorded only with the Klebsiella stimulated MNC. Increased immunoglobulin levels and autoantibody reactivity were noted with all 5 polyclonal activators. The Klebsiella cell membrane preparation induced the highest values. No correlation was found between the 16/6 levels, autoantibody activity and the absolute concentrations of IgG or IgM. This study, together with previous reports, suggests a role for Klebsiella in the etiology of autoimmune diseases. We suggest that Klebsiella can induce anti-DNA-like autoantibodies not only by polyclonal activation, but also by a more specific stimulus.
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PMID:Preferential secretion of a common anti-DNA idiotype (16/6 Id) and anti-polynucleotide antibodies by normal mononuclear cells following stimulation with Klebsiella pneumoniae. 352 98

RU 41.740, a glycoprotein extract from Klebsiella pneumoniae, was seen to activate human B cells to immunoglobulin secretion in vitro. The effects of RU 41.740 on human B cells were compared to those induced by pokeweed mitogen, a T-cell-dependent polyclonal B-cell activator, and Epstein-Barr virus, a T-cell-independent polyclonal B-cell activator. Exposure of human B cells to all of these agents resulted in increased immunoglobulin M (IgM) and immunoglobulin G (IgG) secretion. IgM and IgG secretion induced by RU 41.740 appeared to be T cell dependent when B cells were isolated from human peripheral blood. However, this activity may have been T cell independent when B cells were isolated from human spleen. RU 41.740-induced IgM secretion by peripheral blood B cells was seen to peak after 6 days in culture; IgG secretion peaked after 7 days in culture. The optimal concentration of RU 41.740 for the induction of IgM and IgG secretion by human B cells in vitro was seen to be 200 micrograms/ml.
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PMID:Immunoglobulin M and immunoglobulin G secretion by human B cells exposed to RU 41.740, a glycoprotein extract from Klebsiella pneumoniae. 388 Nov 93

In this paper, we report the presence on Epstein-Barr virus-transformed lymphoblastoid cell lines on platelets and on fibroblasts of an HLA-B27-associated cell surface complex (antigenically related to some antigens of Klebsiella K43 and K21) which is identical to or cross-reactive with the determinant present on the peripheral blood lymphocytes (PBL) of B27-positive patients with ankylosing spondylitis (AS). By contrast, no Klebsiella K43 markers could be demonstrated on the spermatozoa of B27+ AS+ individuals even though these cells expressed the HLA-B27 alloantigen. No B27-associated K43 antigen was detected on the erythrocytes of patients or of normal controls. The B27-associated membrane marker is still detectable on lymphoblastoid cell lines after 20 generations and on fibroblasts after about 10 generations. This finding implies that the continued expression of Klebsiella-modified B27 structure is generally determined and does not require the repeated exposure of the cell surface to Klebsiella antigen. These data suggest that certain non-lymphoid as well as lymphoid cells may be involved in the complex sequence of events leading to the clinical manifestation of AS.
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PMID:The distribution of a specific HLA-B27-associated cell surface component on the tissues of patients with ankylosing spondylitis. 617 70

Epstein-Barr virus transformed lymphoblastoid cell lines from HLA-B27 positive individuals with ankylosing spondylitis (B27+AS+) release, into the culture medium, a factor capable of specifically modifying the HLA-B27 positive lymphocytes of normal individuals (B27+AS-); this modification results in a phenotypic change similar to that seen on B27+AS+ lymphocytes. This lymphoblastoid cell line derived factor appears to be physically and functionally similar to a factor present in the culture filtrate of certain Klebsiella isolates. Biogel P-100 chromatography of the material released from the cell line indicated a mol.wt of 25,000-30,000, similar to that of the Klebsiella derived factor. Chromatofocusing on a PBE 94 column revealed that cell line derived factor had an isoelectric point of 5.5 (cf. pI 5.4 for the Klebsiella derived factor). Immunoadsorption experiments suggest that the factor from the B27+AS+ cell line shares antigenic determinants with a cell surface component present on certain Klebsiella isolates. These results will form the basis for future studies on the nature of the interaction between HLA-B27 and certain enteric organisms and their products. A better understanding of this association should elucidate some of the early events in the pathogenesis of the seronegative arthropathies.
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PMID:A factor shed by lymphoblastoid cell lines of HLA-B27 positive patients with ankylosing spondylitis, specifically modifies the cells of HLA-B27 positive normal individuals. 619 93


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