Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0519030 (
Klebsiella
)
21,988
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background:
Parkinson's disease (PD) is characterized by loss of dopaminergic neurons and intraneuronal accumulation of alpha-synuclein, both in the basal ganglia and in peripheral sites, such as the gut. Peripheral immune activation and reactive oxygen species (ROS) production are important pathogenetic features of PD. In this context, the present study focused on the assessment of
in vitro
effects of probiotic bacterial strains in PBMCs isolated from PD patients vs. healthy controls.
Methods:
40 PD patients and 40 matched controls have been enrolled. Peripheral blood mononuclear cells (PBMCs) were isolated and co-cultured with a selection of probiotics microorganisms belonging to the
lactobacillus
and
bifidobacterium
genus.
In vitro
release of the major pro- (Tumor Necrosis Factor-alpha and Interleukin-17A and 6) and anti-inflammatory (
Interleukin 4
and 10) cytokines by PBMCs, as well as the production of ROS was investigated. Furthermore, we assessed the ability of probiotics to influence membrane integrity, antagonize the growth of potential pathogen bacteria, such as
Escherichia coli
and
Klebsiella
pneumoniae
and encode tyrosine decarboxylase genes (
tdc
).
Results:
All probiotic strains were able to inhibit inflammatory cytokines and ROS production in both patients and controls. The most striking results were obtained in PD subjects with
L. salivarius
LS01 and
L. acidophilus
which significantly reduced pro-inflammatory and increased the anti-inflammatory cytokines (
p
< 0.05). Furthermore, most strains determined restoration of membrane integrity and inhibition of
E. coli
and
K. pneumoniae
. Finally, we also showed that all the strains do not carry
tdc
gene, which is known to decrease levodopa bioavailability in PD patients under treatment.
Conclusions:
Probiotics exert promising
in vitro
results in decreasing pro-inflammatory cytokines, oxidative stress and potentially pathogenic bacterial overgrowth.
In vivo
longitudinal data are mandatory to support the use of bacteriotherapy in PD.
...
PMID:Probiotics May Have Beneficial Effects in Parkinson's Disease:
In vitro
Evidence. 3113 68
