Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0476089 (endometrial cancer)
 11,379 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A new monoclonal antibody, 1C5, was produced by fusion of spleen cells obtained from mice immunized with CAC-1, a human cell line of cervical adenocarcinoma of the uterus, and NS-1 myeloma cell. The objectives of this study were to obtain moAb that can be used for routine histology and cytology, and to examine the histogenesis of cervical adenocarcinoma. 1. 1C5 reacted with 88% of cervical adenocarcinoma of the uterus, but did not react with cervical squamous cell carcinoma of the uterus and other squamous cell carcinoma. However, 1C5 reacted with some adenocarcinomas, such as endometrial carcinoma of the uterus and ovarial carcinoma. 2. The staining pattern by 1C5 was different, in cervical adenocarcinoma from that in endometrial carcinoma of the uterus, and also different in the endocervical type from that in the endometrioid type of cervical adenocarcinoma. Therefore, 1C5 is useful in distinguishing between two types of adenocarcinoma of the uterus. 3. 1C5 did not react with normal squamous cells or normal columnar cells of the uterine cervix, or with normal endometrial cells of the uterus. However, the columnar cells in a limited area of the squamocolumnar junction were strongly stained with 1C5. 4. 1C5 reacted with ethanol-fixed, and routine formalin-fixed and paraffin-embedded tissue. Thus, 1C5 may be used for clinical diagnosis. 5. 1C5 was found to be IgG1. 6. The molecular weight of the 1C5-defined antigen was 26,000 daltons, and the epitope of the 1C5-defined antigen was carbohydrate moiety. 7. We examined the histogenesis of cervical adenocarcinoma of the uterus by utilizing the reactivity of 1C5.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[The production and characterization of monoclonal antibody, 1C5, reactive with cervical adenocarcinoma of the uterus]. 247 40

A murine monoclonal antibody, 1C5, was produced by fusion of spleen cells obtained from mice immunized with CAC-1, a human cell line of adenocarcinoma derived from uterine cervix, and NS/1 myeloma cells. 1C5 can be used for the staining of routine formalin-fixed and paraffin-embedded tissue sections. 1C5-defined antigen was found to have a molecular weight of 26,000. The 1C5-defined antigen was resistant to neuraminidase and trypsin treatment, but sensitive to periodate treatment, indicating that an epitope of the 1C5-defined antigen is a carbohydrate moiety. Immunohistochemical study using immunoperoxidase staining demonstrated that 1C5 reacted with 87% of adenocarcinomas of the uterine cervix, 39% of endometrial carcinomas of the uterus, 100% of ovarian mucinous cystadenocarcinomas, 43% of ovarian serous cystadenocarcinomas, 45% of adenocarcinomas of the colon, and 40% of gastric adenocarcinomas, thus showing the broad reactivity to adenocarcinoma cells of various origins. However, 1C5 did not show any reactivity to ectocervix epithelium, cervical intraepithelial neoplasia, or squamous cell carcinoma of the uterine cervix. In addition, adenocarcinoma of the uterine cervix exhibited strong cytoplasmic reactivity with 1C5, whereas endometrial carcinoma of the uterus showed the luminal reactivity. 1C5 also reacts with 95% ethanol-fixed malignant cells in cervical smears.
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PMID:New monoclonal antibody, 1C5, reactive with human cervical adenocarcinoma of the uterus, with immunodiagnostic potential. 305 7

Voltage-gated potassium (Kv) channels play important roles in differentiation and growth of non-excitable cells. Inhibition of these channels is also known to suppress proliferation of various cancer cells. Here we examine expression of K+ channel subunit genes in various uterine cancer cells and their roles in cell proliferation. RT-PCR analysis reveals that cervical squamous cell carcinoma (C-33A, MS-751 and QG-U), and endometrial adenocarcinoma (AN3-CA, KLE and Ishikawa), but not cervical adenocarcinoma (CAC-1 and OMC-4), expresses both or either one of the two human eag-related genes (HERG2 and 3, or KCNH6 and 7). In addition, mRNAs for one-transmembrane auxiliary subunits (KCNE1-3) are significant in these cells. Moreover, the two cervical adenocarcinoma cell lines, as well as some of the squamous and endometrial cancer cell lines, express mRNAs for Kv2.1 and the silent regulatory subunit for Kv2.1 channels, Kv9.3. Thus, squamous/endometrial cancer cells contain HERG-KCNE channel complexes, whereas Kv2.1-Kv9.3 channels may be major components of Kv channels in cervical adenocarcinoma cells. To evaluate the involvement of these channels in cell proliferation, we used the specific blockers for HERG and Kv2.1-containing channels, E-4031 and hanatoxin-1. E-4031 significantly reduced proliferation of C-33A, MS-751 and QG-U by 15-30%. Similarly, hanatoxin-1 suppressed growth of Kv2.x-expressing cells (25-40%). Finally, FACS analysis indicates that inhibition of HERG channels reduces a population of cells in the G2/M phase. These results suggest that HERG-KCNE and Kv2.1-Kv9.3 channels are selectively involved in proliferation of distinct uterine cancer cells.
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PMID:Selective expression of HERG and Kv2 channels influences proliferation of uterine cancer cells. 1520