Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0451641 (urolithiasis)
 3,973 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The prevalence of urolithiasis in the general population has been increasing recently. The inflammatory responses may play an important role in the development of urolithiasis. We aimed to investigate whether the urine inflammatory cytokine and chemokine profiles from patients with urolithiasis can be used as prognostic markers for urolithiasis. Multiplex immunoassays were used to simultaneously detect five inflammatory cytokines and five inflammatory chemokines in urine collected from 29 patients and 38 sex and age-matched healthy volunteers. After adjusting for urinary creatinine, urinary levels of interleukin-8 (IL-8), regulated on activation, normal T cell expressed and secreted, monocyte chemoattractant protein-1, interferon-gamma (IFN-gamma)-inducible 10-kDa protein, monokine induced by IFN-gamma and IL-6 were significantly increased in patients compared with healthy controls. However, concentrations of urinary IL-1beta, IL-10, IL-12, and tumor necrosis factor-alpha were not significantly different between those of patients and healthy controls. Using receiver operating characteristics curve analysis, we found that the adjusted IL-8 level of 6.2 pg/mg creatinine can reach a sensitivity of 90% and specificity of 68% to detect urolithiasis. Our data showed that urinary stones are associated with a cascade of inflammatory responses, including chemokine secretion, and urinary IL-8 levels. In addition, the elevation of urinary IL-8 could be a useful biomarker in healthy screening and clinical follow-up of urolithiasis.
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PMID:Urinary chemokines/cytokines are elevated in patients with urolithiasis. 2020 39

The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and stone-forming group. 24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks. MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles. To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed. A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group. Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway. Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination. The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism.
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PMID:Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model. 2939 39