Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0432222 (SEM)
 47,337 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cardiac troponin T (cTnT) is a highly sensitive and specific serum marker of irreversible cardiomyocyte injury. It is not clear whether cTnT also is a suitable marker of subtle, reversible injury. In the present investigation the relationship between cTnT release and function during the first 30 min of reperfusion after 30 min of global ischaemia, was investigated in isolated, retrogradely perfused rat hearts. Left ventricular systolic (LVSP), end-diastolic (LVEDP) and developed (LVDP) pressures, heart rate (HR), and coronary flow (CF) were measured. In one series of experiments (n=7) the kinetics of cTnT release during 30 min of reperfusion was investigated. An early, short-lasting peak of cTnT release appeared after 30 s of reperfusion. Then cTnT release gradually increased with a maximum after 20 min (from 0.08 +/- 0.03 before ischaemia to 2.16 +/- 0.40 ng min-1) (mean +/- SEM). In a second series of experiments (n=52) the relationship between cTnT release and cardiac function was investigated after 20 min of reperfusion. At this time point LVEDP increased (0 to 62 +/- 3 mmHg) and LVDP decreased (84 +/- 2 to 33 +/- 3 mmHg), but without any correlation with cTnT release. cTnT release was positively correlated to LVSP (P < 0.04, r=0.29), and negatively correlated to HR (P < 0.03, r=-0.31). cTnT concentration in the coronary effluent increased in parallel to increasing CF (P < 0.03, r=0.31). In conclusion, during the early reperfusion period there was no consistent correlation between cTnT release and dysfunction after global ischaemia in the isolated rat heart. Release of cTnT and post-ischaemic function appear to provide supplementary information in this particular model.
 ...
PMID:Post-ischaemic dysfunction does not correlate with release of cardiac troponin T in isolated rat hearts. 1051 73

Cardiac troponin T (cTnT) levels are widely used to assess for evidence of myocardial infarction. We studied the effect of freezing and long-term storage on the stability of cTnT in blood samples from 178 patients with end-stage renal failure. The serum was separated and divided into multiple aliquots. Baseline cTnT levels were measured in the unfrozen aliquots. The remaining aliquots were frozen using standard techniques. The aliquots were thawed after 3, 6, 12, or 24 months and cTnT levels measured. There were no significant changes in the mean +/- SEM cTnT levels up to 12 months (0.111 +/- 0.098 microg/L) compared with baseline (0.114 +/- 0.098 microg/L); after 24 months, cTnT levels were significantly lower (0.107 +/- 0.095 microg/L) than baseline ( P = .004). The cTnT assay is a reliable method of measuring the cTnT level in human serum up to 12 months of frozen storage. However, after 24 months, the cTnT level was 0.007 microg/L lower than baseline, potentially causing erroneous interpretations. The clinical significance of the change in the cTnT level after long-term frozen storage is unclear. Further studies, including prospective analysis of patient outcomes, should be helpful.
 ...
PMID:The effect of freezing and long-term storage on the stability of cardiac troponin T. 1758 Feb 85