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 47,337 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this study, rat periosteal cells and bone marrow stromal cells (BMSC) were examined when cultured on octacalcium phosphate (OCP) crystals to determine the effects of crystal morphology on their adhesion, spreading, and growth. Three type of OCP crystals with different morphology were prepared by different precipitation methods. OCPI was prepared by homogeneous precipitation; while OCPII and III were prepared by heterogeneous precipitation dripping a calcium ion containing solution into a phosphate ion solution or vice versa, respectively. The effects of crystal morphology on the attachment and spreading of cultured cells on OCP crystals were observed using SEM. The effects of crystal morphology on the growth of periosteal cell or BMSC were evaluated using the MTT assay, while OCP crystal cytotoxicity was determined by measuring lactic dehydrogenase (LDH) released from 2.12 x 10(5) cells incubated with 60 mg of the different OCPs. BMSCs and periosteal cells attached to or between the crystals of OCPI and the cell number increased over the 17 days of culture. In contrast, cell numbers could not be measured for either periosteal cells or BMSC on OCPII and III. BMSC incubated with OCPII and III showed a higher rate of LDH release, compared with the cells incubated with OCPI. This study demonstrated that the morphology of OCP crystals influenced the attachment, spreading and growth of periosteal cells and BMSC.
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PMID:Influence of calcium phosphate crystal morphology on the adhesion, spreading, and growth of bone derived cells. 1865 45

Bioactive glasses (BaG) can bind to human bone tissues and have been used in many biomedical applications for the last 30 years. However they usually are weak and brittle. On the other hand, composites that combine polymers and BaG are of particular interest, since they often show an excellent balance between stiffness and toughness. Bioactive glass-poly(vinyl alcohol) foams to be used in tissue engineering applications were previously developed by our group, using the sol-gel route. Since bioactive glass-polymer composite derived from the sol-gel process cannot be submitted to thermal treatments at high temperatures (above 400 degrees C), they usually have unreacted species that can cause cytotoxicity. This work reports a technique for stabilizing the sol-gel derived bioactive glass/poly(vinyl alcohol) hybrids by using glutaraldehyde (GA), NH(4)OH solutions and a blocking solution containing bovine serum albumin. PVA/BaG/GA hybrids were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM/EDX) analyses. Moreover, MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) biocompatibility and cytotoxicity assays were also conducted. The hybrids exhibited pore size varying from 80 to 820 mum. After treatments, no major changes in the pore structure were observed and high levels of cell viability were obtained.
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PMID:Synthesis, neutralization and blocking procedures of organic/inorganic hybrid scaffolds for bone tissue engineering applications. 1880 51

In the present work we report the synthesis, characterization, and preliminary biocompatibility of polymer blends based on Chitosan and poly(vinyl alcohol) (PVA) with low degree of hydrolysis and chemically crosslinked by glutaraldehyde for potential application on skin tissue repairing. The microstructure and morphology of the blended hydrogels were characterized through Fourier Transform Infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM/EDX) analysis. Hydrogels were investigated by swelling as preliminary in vitro test using simulated body fluid. In addition, biocompatibility, cytotoxicity, and cell viability were assessed via MTT assay with VERO cell culture and cell spreading-adhesion analysis. It was found that by increasing the chitosan to PVA ratio, simulated body fluid uptake of the material was significantly altered. All the tested hydrogels have clearly presented adequate cell viability, non-toxicity, and suitable properties which can be tailored for prospective use in skin tissue engineering.
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PMID:Properties and biocompatibility of chitosan films modified by blending with PVA and chemically crosslinked. 1898 49

The fabrication of biodegradable 3-D scaffolds enriched with multipotent stem cells seems to be a promising strategy for the repair of irreversibly injured tissues. The fine mechanisms of the interaction of rat mesenchymal stem cells (rMSCs) with a hyaluronan-based scaffold, i.e. HYAFF(R)11, were investigated to evaluate the potential clinical application of this kind of engineered construct. rMSCs were seeded (2 x 10(6) cells cm(-2)) on the scaffold, cultured up to 21 days and analysed using appropriate techniques. Light (LM), scanning (SEM) and transmission (TEM) electron microscopy of untreated scaffold samples showed that scaffolds have a highly porous structure and are composed of 15-microm-thick microfibres having a rough surface. As detected by trypan blue stain, cell adhesion was high at day 1. rMSCs were viable up to 14 days as shown by CFDA assay and proliferated steadily on the scaffold as revealed by MTT assay. LM showed rMSCs in the innermost portions of the scaffold at day 3. SEM revealed a subconfluent cell monolayer covering 40 +/- 10% of the scaffold surface at day 21. TEM of early culture showed rMSCs wrapping individual fibres with regularly spaced focal contacts, whereas confocal microscopy showed polarized expression of CD44 hyaluronan receptor; TEM of 14-day cultures evidenced fibronexus formation. Immunohistochemistry of 21-day cultures showed that fibronectin was the main matrix protein secreted in the extracellular space; decorin and versican were seen in the cell cytoplasm only and type IV collagen was minimally expressed. The expression of CD90, a marker of mesenchymal stemness, was found unaffected at the end of cell culture. Our results show that HYAFF(R)11 scaffolds support the adhesion, migration and proliferation of rMSCs, as well as the synthesis and delivery of extracellular matrix components under static culture conditions without any chemical induction. The high retention rate and viability of the seeded cells as well as their fine modality of interaction with the substrate suggest that such scaffolds could be potentially useful when wide tissue defects are to be repaired as in the case of cartilage repair, wound healing and large vessel replacement.
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PMID:Mesenchymal stem cell interaction with a non-woven hyaluronan-based scaffold suitable for tissue repair. 1901 59

Bioactive borosilicate glass scaffolds with the pores of several hundred micrometers and a competent compressive strength were prepared through replication method. The in vitro degradation and bioactivity behaviors of the scaffolds have been investigated by immersing the scaffolds statically in diluted phosphate solution at 37 degrees C, up to 360 h. To monitor the degradation progress of the scaffolds, the amount of leaching elements from the scaffolds were determined by ICP-AES. The XRD and SEM results reveal that, during the degradation of scaffolds, the borosilicate scaffolds converted to hydroxyapatite. The compressive strength of the scaffolds decreased during degradation, in the way that can be well predicted by the degradation products, or the leachates, from the scaffolds. MTT assay results demonstrate that the degradation products have little, if any, inhibition effect on the cell proliferation, when diluted to a certain concentration ([B] <2.690 and pH value at neutral level). The study shows that borosilicate glass scaffold could be a promising candidate for bone tissue engineering material.
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PMID:Bioactive borosilicate glass scaffolds: in vitro degradation and bioactivity behaviors. 1918 71

A polyethersulfone (PES) membrane was modified by blending with a co-polymer of acrylic acid (AA) and N-vinyl pyrrolidone (VP), followed by immobilization of bovine serum albumin (BSA) onto the surface. The scanning electron microscopy results showed that PES had good miscibility with the co-polymer. X-ray photoelectron spectroscopy confirmed the existence of P(VP-AA) co-polymer on the surface of the blended membrane and the existence of BSA after the immobilization process. The amount of BSA immobilized on the surface of the membranes was determined. It was found that the protein adsorption amounts from BSA, human plasma fibrinogen and diluted human plasma solutions decreased significantly after modification. According to the circular dichroism results, the proteins kept more alpha-helix conformation in the modified membranes than in the pure PES membrane. The number of the adhered platelets was reduced, and the morphology change for the adherent platelets was also suppressed by the modification with BSA. The SEM morphological observation of the cells and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay demonstrated that the BSA-modified PES membrane surface promoted endothelial cell adhesion and proliferation.
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PMID:BSA-modified polyethersulfone membrane: preparation, characterization and biocompatibility. 1919 62

The attachment, growth behaviour and the genetic effect of human gingival fibroblasts (HGF) cultured on titanium and different zirconia surfaces were investigated. HGF cells were cultured on (1) titanium discs with a machined surface, (2) yttrium-stabilized tetragonal zirconia polycrystals (Y-TZP) with a smooth surface and (3) Y-TZP with 100 microm grooves. The cell proliferation activity was evaluated through a MTT assay at 24 h and 48 h, and the cell morphology was examined by SEM. The mRNA expression of integrin-beta1, type I and III collagen, laminin and fibronectin in HGF were evaluated by RT-PCR after 24 h. From the MTT assay, the mean optical density values for the titanium and grooved zirconia surfaces after 48 h of HGF adhesion were greater than the values obtained for the smooth zirconia surfaces. SEM images showed that more cells were attached to the grooves, and the cells appeared to follow the direction of the grooves. The results of RT-PCR suggest that all groups showed comparable fibroblast-specific gene expression. A zirconia ceramic surface with grooves showed biological responses that were comparable to those obtained with HGF on a titanium surface.
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PMID:Attachment and growth behaviour of human gingival fibroblasts on titanium and zirconia ceramic surfaces. 1920 38

In this study, a new hydroxyapatite (HA)/polyurethane (PU) composite porous scaffold was developed by in situ polymerization. Aliphatic isophorone diisocyanate as a nontoxic and safe agent was adopted to produce the rigid segment in polyurethane polymerization. Hydroxyapatite powder was compounded in a PU polymer matrix during the polymeric process. The macrostructure and morphology as well as mechanical strength of the scaffolds were characterized by FTIR, XRD, DSC and SEM. The results show that the isophorone diisocyanate can react mildly with hydroxyl (-OH) groups of castor oil and a mild foaming action caused by the release of CO2 gas occurred simultaneously in the reactive process, thus producing a uniform porous structure of HA/PU scaffold. The HA/PU composite scaffold with a high HA content of about 60 wt% has a porosity of more than 78% and a pore size from 100 microm to 800 microm. The HA/PU scaffold exhibited good cytocompatibility estimated by co-culturing the scaffold with MG63 cells through MTT test. The porous composite scaffold has good homogenization and a perfect three-dimensional structure for cell migration and bone tissue ingrowth, and should have good prospects for bone tissue regeneration.
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PMID:Porous bioactive scaffold of aliphatic polyurethane and hydroxyapatite for tissue regeneration. 1920 42

Fiber mesh scaffolds were recently investigated in tissue engineering as possible support for stem cell growth and differentiation, in order to repair lesion areas in clinical practice. In particular, the literature is focused on fiber mesh scaffolds constituted of biocompatible and resorbable polymeric structures, like poly(L-lactic acid) (PLLA). However, as regards the study of constructs constituted of PLLA microfibers and cells, only quantitative and SEM analyses were reported, lacking histological analysis. Histological evaluation of these constructs could give important information about cellular distribution in the scaffold, cell-scaffold interactions and extracellular matrix production. The purpose of our study was to find a valid method to analyze PLLA microfiber/cell constructs from both histological and histochemical angles. Biodegradable non-woven fiber meshes were prepared using hollow microfibers, based on PLLA. We first evaluated different embedding methods useable for histological analysis and the results showed that among the paraffin, Killik, and acrylic resin the only suitable medium was the latter. Then we employed the acrylic resin to embed the constructs made up of PLLA microfibers and bone marrow-derived human mesenchymal stromal cells, which we then analyzed with Toluidine Blue, PAS and Alcian Blue staining. These constructs, previously analyzed for cell viability by MTT and CCK-8 tests, showed viable/proliferating cells until 6 weeks of culture. The stainings performed on constructs confirmed viability data obtained with SEM and MTT/CCK-8 and supplied other information on the cell behaviors such as the distribution and organization onto the scaffold and the production of extracellular matrix molecules. In conclusion, this methodological study mainly suggests a suitable method to analyze PLLA microfiber/cell constructs, at the same time confirming and enriching the literature data on the compatibility between PLLA microfibers and hMSCs.
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PMID:Embedding methods for poly(L-lactic acid) microfiber mesh/human mesenchymal stem cell constructs. 1933 88

Zerumbone (ZER), a potential anticancer compound, isolated from the fresh rhizomes of Zingiber zerumbet. In this investigation, the cytotoxic properties of ZER were evaluated, on cancer cells of human cervix (HeLa), breast and ovary, and normal cells of Chinese Hamster ovary, using MTT assay. Apoptogenic effects of ZER on HeLa were studied using fluorescence microscopy (AO/PI double staining), scanning and transmission electron microscopy (SEM and TEM), and colorimetric assay of the apoptosis promoter enzyme, caspase-3. The results of MTT assay showed that ZER has less effect on normal cells compared to cancer cells. The lowest IC(50) of ZER was observed on HeLa cells. Cytological observations showed nuclear and chromatin condensation, cell shrinkage, multinucleation, abnormalities of mitochondrial cristae, membrane blebbing, holes, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double staining of AO/PI, SEM and TEM. Statistical analysis (two-tailed t-test) of differential counting of 200 cells under fluorescence microscope revealed significant difference in apoptotic cells populations between treated and untreated HeLa cells. In addition, ZER has increased the cellular level of caspase-3 on the treated HeLa cells. It could be concluded that ZER was able to produce distinctive morphological features of cell death that corresponds to apoptosis.
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PMID:In vitro ultramorphological assessment of apoptosis induced by zerumbone on (HeLa). 1934 71


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