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 47,337 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study developed a new technique to quantitate platelets adhered on biomaterials surfaces in vitro, based on a surface phased radioimmunoassay using a monoclonal antibody SZ-21, directed specifically against the membrane glycoprotein complex IIIa of human platelets. In vitro perfusion is performed in system which consists of testing tubes and infusion pump. After 5 minutes perfusion with fresh ACD anticoagulated human whole blood at 2,000s-1 platelets deposition on surface precoated with proteins determined using anti-human platelet antibody (125 I-SZ-21) are 4,173 +/- 932 (Albumin), 59,032 +/- 25,554 (Fibrinogen), and 71,253 +/- 11,484 (Collagen). Meanwhile, platelets adhered on surfaces of four polymers were determined (platelet/mm2): 19,493 +/- 2,050 (Silicone), 48,193 +/- 4,055 (Polytetrafluoroethylene), 50,375 +/- 8,675 (Polyvinyl chloride) and 101,906 +/- 5,916 (Polyethylene). These results were confirmed by SEM. This method is not only applied for evaluating rapidly and reliably blood compatibility of biomaterials in vitro, but will be used at basic study for interaction of blood materials.
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PMID:New method to quantitate platelets adhered on biomaterials using monoclonal antibodies to human platelet membrane glycoprotein SZ-21. 238 68

Regeneration of 2,3-diphosphoglycerate (DPG) was determined following transfusion of DPG-depleted group O red cells into group A recipients. Blood from five donors was stored in the adenine-containing solutions CPDA-1, AS-1 or AS-3 for 35 d at 4 degrees C. Post-transfusion red cell DPG and ATP were measured in separated group O red cells over a 7 d period. The studies confirmed rapid in vivo DPG regeneration with greater than or equal to 50% of the maximum level being achieved within 7 h. An average of 95% of the recipients' pre-transfusion DPG level was achieved by 72 h and by 7 d mean (+/- SEM) DPG levels relative to recipient's pre-transfusion DPG averaged 84% (+/- 13%), 92% (+/- 17%) and 84% (+/- 21%) for CPDA-1, AS-1 and AS-3 red cells, respectively. Results were comparable to those previously reported for blood stored in ACD for 15-20 d (Valeri & Hirsch, 1969; Beutler & Wood, 1969). The immediate regeneration rate, V, closely approximated first order regeneration kinetics with AS-3 red cells exhibiting double the rate of CPDA-1 red cells (P less than 0.001). AS-1 red cells exhibited an intermediate rate of regeneration which was not significantly different compared to either CPDA-1 or AS-3 (P greater than 0.05). V exhibited a significant (P less than 0.05) positive correlation with ATP levels 5-7 h post-infusion. ATP regeneration of the infused cells was rapid with a mean increase of 1.2 mumol/g Hb above post-storage levels being achieved 1 h following transfusion.
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PMID:In vivo regeneration of red cell 2,3-diphosphoglycerate following transfusion of DPG-depleted AS-1, AS-3 and CPDA-1 red cells. 249 18

In this study, sorption of copper onto low molecular weight chitosan derivative was studied. Experimental parameters such as pH of the solution (A), temperature (B), dose of the sorbent (C), and concentration of solution (D) were considered. The statistical results indicated that the dose of sorbent (C) and Cu (II) concentration (D) influenced removal efficiency at 5% significance level. Also, some interactions such as ABCD, ACD, ABC and AC affected the removal process. The sorbent was characterized with FTIR, SEM and TG/DSC. Freundlich isotherm model was the best isotherm model. The kinetic study results correlated well with the pseudo-second-order model. The thermodynamic studies revealed that the nature of copper sorption was spontaneous and endothermic. Strong affinity of the sorbent for copper (II) was revealed by the Isothermal Titration Calorimetry (ITC) technique.
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PMID:Sorption of copper onto low molecular weight chitosan derivative from aqueous solution. 2703 44