Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0432222 (SEM)
 47,337 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The development of a new technique to investigate maternal-fetal transfer across the near term guinea-pig yolk sac placenta by in-situ perfusion of the yolk sac vessels is described. The maternal-fetal transfer of labeled water, D- and L-glucose, O-methyl-D-glucose (oMDG), D- and L-alanine, D- and L-aspartate, L-lactate and alpha-amino-isobutyric acid (AIBA) was investigated after injection of these substances into the maternal circulation. After 15 min of perfusion at 0.5 ml/min the water clearance was 132 +/- 12 microliters/min (SEM, n = 30). The clearances for D- or L-glucose were less than 1.2 microliters/min. The activity of label in the venous yolk sac perfusate of all other substances was not different from background activity when 14C-label was used. The clearance of 3H-L-alanine approached the clearance value of water. The total uptake (as defined for single-injection double tracer dilution experiments) from the perfusate of D-glucose, oMDG, alanine and aspartate in comparison to L-glucose was also studied. Mean D-glucose uptake was 11.2 +/- 1.9 percent (n = 8), it was significantly reduced to 4.9 +/- 2 percent (n = 5) by cytochalasin B (1 X 10(-4) mmol/l), and by increasing concentrations of D-glucose (1 to 20 mmol/l, n = 4). The uptake of oMDG was 8.8 +/- 1.5 percent (n = 8). L-alanine uptake was 25 +/- 3.4 percent, D-alanine uptake was 8.3 +/- 1.5 percent (n = 12). Both uptake values were decreased significantly by 10 mmol/l L-alanine, but unaffected by [Na+] (less than 15 mequ/l). There was no uptake of AIBA. The uptakes of L-aspartate were 34.9 +/- 3.7 percent and of D-aspartate 40.4 +/- 4.8 percent (n = 11). Both uptake values were significantly and reversibly reduced by 1 mmol/l L-aspartate and D-aspartate, and by low [Na+] (less than 15 mequ/l). It is concluded that water can move by diffusion from maternal circulation into the yolk sac capillaries in considerable amounts whereas the contribution of the yolk sac placenta to fetal nutrition with D-glucose, L-alanine and L-aspartate is negligible. The membranes of yolk sac cells contain specific transport systems for D-glucose, D-/L-alanine and D-/L-aspartate transfer. The function of the vitelline placenta in the near-term guinea-pig is comparable more to the gut than to the chorio-allantoic placenta.
Placenta
PMID:The artificially perfused guinea-pig yolk sac placenta: transfer and uptake of water, glucose and amino acids. 177 43

Villi from human, macaque and baboon placentae were subjected to ultrasonication after prolonged osmication, and examined by scanning electron microscopy. The technique was often successful in removing the overlying trophoblast and revealing expanses of the trophoblastic basal lamina, a conclusion corroborated by transmission electron microscopy. These preparations bore a remarkable similarity in appearance to microvascular cast preparations of the fetal vasculature. Relatively straight parallel tubules appeared to correspond in position to the location of fetal vessels in intermediate villi, whereas portions of the basal laminae of terminal villi were in the form of convoluted, branched cylinders similar to SEM images of fetal capillaries of terminal villi. The basal lamina did not have evidence of pores as has been described in some basal laminae.
Placenta
PMID:Scanning electron microscopy of primate chorionic villi following ultrasonic microdissection. 203 97

Elevated levels of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are found in late pregnancy but the factors responsible for this are not known. To determine if the maternal-fetal calcium flux or the presence of a previously described extrarenal 25-hydroxycholecalciferol-1-hydroxylase (25(OH)-D3-1-hydroxylase) play a role, serum calcium and 1,25(OH)2D3 were measured in pregnant, nonpregnant, and decidua-bearing pseudopregnant rats. Serum calcium was 8.74 +/- 0.26 mg/dl (mean +/- SEM) in nonpregnant rats. In pregnant rats, serum calcium was not significantly different from nonpregnant controls on day 12 and only slightly higher on day 15. Pseudopregnant rats were significantly hypercalcemic on days 12 (11.93 +/- 0.19 mg/dl) and 15 (11.45 +/- 0.23 mg/dl) compared with nonpregnant rats (P less than 0.001). In nonpregnant controls the serum level of 1,25(OH)2D3 was 44.6 +/- 6.3 pg/ml. Levels in pregnant rats were not significantly different on days 12 or 15 but tended to be higher by day 15 (75.2 +/- 19.7 pg/ml). Pseudopregnant rats had levels of 72.6 +/- 13.5 pg/ml on day 12 and 102.8 +/- 10.9 pg/ml on day 15, the latter of which was significantly higher than nonpregnant values (P less than 0.05). 25(OH)D3-1-hydroxylase activity was determined in whole tissue homogenates of placenta and decidua. Placenta from pregnant rats and decidua from pregnant and pseudopregnant rats both formed putative 1,25(OH)2D3 in short-term incubation with 25(OH)D3 as identified by comigration with authentic 1,25(OH)2D3 on high pressure liquid chromatography (HPLC).(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:Vitamin D metabolism in pregnant and pseudopregnant rats: identification of 25-hydroxycholecalciferol-1-hydroxylase in decidual tissue. 313 6

Human placenta is the major source of activin A in maternal circulation. The aim of the present study was to evaluate maternal activin A serum concentration in pregnant women with chronic hypertension (n = 14), pregnancy-induced hypertension (n = 10) or pre-eclampsia (n = 16). In the group of pregnant women with chronic hypertension and of healthy pregnant women (n = 10) activin A was measured in samples collected longitudinally throughout gestation. Using a specific two-site enzyme-linked immunosorbent assay, it has been possible to measure maternal serum activin A concentration. In addition, the effect of recombinant human activin A administration on mean arterial pressure and heart rate in female rats have been also investigated. Mean +/- SEM of maternal serum activin A concentration in pre-eclamptic women (57.4 +/- 28.3 ng/ml), was significantly higher than in women with pregnancy-induced hypertension (14.8 +/- 10.5 ng/ml), chronic hypertension (10.3 +/- 5.4 ng/ml) or healthy control women (9.2 +/- 9.4 ng/ml) (P < 0.01). Serum activin A levels evaluated 2 weeks after anti-hypertensive treatment were not significantly different in pre-eclamptic women. Moreover, when exogenous recombinant human activin A was administered in female rats arterial pressure or frequency of heart rate did not change. The present study showed that maternal serum activin A concentration is abnormally high in patients with pre-eclampsia. Thus, since the patients with chronic hypertension or pregnancy-induced hypertension have activin A concentration in the normal range of values, activin A may be a prognostic marker of hypertension in pregnancy.
Placenta 1995 Jul
PMID:Hypertension in pregnancy: changes in activin A maternal serum concentration. 747 15

Homogenized first trimester human placenta exhibits both Ca(2+)-dependent (90-95 per cent) and Ca(2+)-independent (5-10 per cent) nitric oxide (NO)-synthesizing activities. Addition of tetrahydrobiopterin (BH4) to homogenates containing Ca2+ in maximally activating concentrations (> 0.5 microM) results in a further 2-2.5-fold activation of NO synthesis, with half-maximal stimulation observed at 26 +/- 8.2 microM BH4 (mean +/- SEM, n = 4). Chelation of Ca2+ in the medium abolishes the stimulatory effect, indicating that only a Ca2(+)-dependent NO-synthase (NOS) isoform is activated by BH4. Based on our previous findings, we suggest that this isoform is the endothelial or Type III NOS. Importantly, BH4 has no significant effect on the Ca2(+)-dependency of NOS activity, the apparent Km values for Ca2+ are comparable in the absence (1.8 +/- 0.4 microM, mean +/- SEM, n = 6) or presence (2.5 +/- 0.6 microM, mean +/- SEM, n = 6) of 50 microM BH4. The BH4 content of these placentae is 207.4 +/- 86.7 pmol/g wet tissue (mean +/- s.d., n = 9), therefore, BH4 added to the homogenate does not simply restore the concentrations that occur endogenously. The results provide the first evidence that in the early human placenta, a constitutively expressed CA 2(+)-dependent NOS isoform is stimulated by exogenous BH4, raising the possibility that BH4 is an important regulator of NOS activity in this tissue. This novel aspect of the NO-generating pathway may have implications in the aetiology and treatment of pregnancy-induced hypertension and pre-eclampsia.
Placenta 1996 Jan
PMID:Calcium-dependent nitric oxide synthesis is potently stimulated by tetrahydrobiopterin in human primordial placenta. 871 Aug 15

We investigated differences in maternal plasma and trophoblast prostaglandin metabolism associated with preterm births. Tissue prostaglandins (PGs) E2 and F2 alpha and the stable plasma PGF2 alpha metabolite, 13,14-dihydro-15-keto-PGF2 alpha, were measured in preterm (< 37 weeks) and term (< or = 37 weeks) births. Amnion PGE2 in preterm (106.1 +/- 15.7 ng/g wet weight tissue; x +/- SEM; n = 37) was lower than in term (176.6 +/- 22.7 ng/g wet weight; x +/- SEM; n = 34, P < 0.02). Placenta PGE2 was lower in preterm (34.7 +/- 19.7 ng/g wet weight; x +/- SEM) than in term (103.3 +/- 28.0 ng/g wet weight; x +/- SEM, P < 0.04). Preterm PGF2 alpha was consistently lower in the amnion (106.8 +/- 17.5 ng/g wet weight) and placenta (102.5 +/- 8.7 ng/g wet weight) than in term amnion (188.2 +/- 24.8 ng/g wet weight; P < 0.01) and placenta (128.9 +/- 7.8 ng/g wet weight; P < 0.03). Chorionic PGE2 and plasma PGF2 alpha metabolite followed this trend but did not reach significance. These findings suggest qualitative and quantitative differences in maternal and trophoblast eicosanoid metabolism between term and preterm parturition.
 ...
PMID:Prostaglandins in selected reproductive tissues in preterm and full-term gestations. 898 26

The placental vascular architecture differs significantly at high altitude from that at sea level in the human and guinea-pig. Four sheep between 137 and 140 days of gestation, kept near sea level throughout gestation, were used as a normoxic control group for comparison of the placental vasculature with 10 other ewes, kept at high altitude (3820 m above sea level; Barcroft Laboratory, White Mountain Research Station, CA, USA). Placentomes from both groups were prepared for histology and scanning electron microscopy of vascular corrosion casts. Singular perfusion of fetal placentae, as well as combined maternal/fetal injection was performed. The influence of long-term hypoxaemia was determined by qualitative and semi-quantitative evaluation of corrosion casts and histological sections. The fetal vessel casts show a distinct difference in the arrangement of vessels of all sizes in response to long-term hypoxaemia. In the control group, stem arteries and veins are straight and parallel. In contrast, this is much less evident in the hypoxaemic group because arterioles and venules branch off the stem vessels more frequently and in an irregular manner. This leads to a capillary bed that is much more dense due to increased branching and capillary coiling. These observations are confirmed by histomorphometry. In the fetal vessels of high altitude sheep placentomes, we observed a decreased number of vascular cross sections (21.6 +/- 4.7 SEM versus 27.7 +/- 4.0 SEM; P = 0.02). However, the average luminal size per cross section (77.9 +/- 10.5 microns2 SEM versus 59.4 +/- 7.4 microns2 SEM; P = 0.004) was increased at high altitude and the percentage of lumina of the total area (5.7 +/- 0.5 SEM versus 5.3 +/- 0.3 SEM; P = 0.09) indicated a trend towards an increase. In maternal vessels of high altitude placentomes, the number of vessel cross sections (6.5 +/- 0.7 SEM versus 6.0 +/- 0.5 SEM; P = 0.2) remained unchanged, whereas the average luminal size (1108 +/- 122 microns2 SEM versus 844 +/- 77 microns2 SEM; P < 0.001) and the percentage of lumina out of the total area (20.9 +/- 1.8 SEM versus 17.5 +/- 1.7 SEM; P < 0.001) were increased. The interhaemal distance appeared to be slightly but not significantly increased at high altitude. These findings indicate that at high altitude the sheep placenta develops an increased materno-fetal absorptive surface to help guarantee substance exchange.
Placenta 1997 Jan
PMID:Term ovine placental vasculature: comparison of sea level and high altitude conditions by corrosion cast and histomorphometry. 903 9

Corticotropin-releasing factor-binding protein (CRF-BP) in pregnant women is measurable in maternal and fetal plasma as well as in amniotic fluid. The concentration of CRF-BP in maternal plasma and amniotic fluid changes significantly at the time of parturition. The aim of the present study was to evaluate fetal plasma CRF-BP levels in women delivering at term or with preterm labour. CRF-BP levels were measured the in umbilical cord plasma of women subdivided into two groups: (1) healthy pregnant women throughout the last 5 weeks of pregnancy either (a) out of labour (n = 21) or (b) at delivery after spontaneous labour (n = 64); and (2) patients with preterm labour (a) gone to delivery (n = 12) or (b) responding to tocolysis (n = 10). In the group of healthy women at term, CRF-BP levels were also measured in maternal plasma. CRF-BP was measurable in all specimens of umbilical cord plasma. Mean values +/- SEM at 40 weeks (5.85 +/- 0.65 nmol/l) were significantly lower than those obtained at 37 (6.48 +/- 0.47 nmol/l) or 38 (6.95 +/- 1.16 nmol/l) weeks of pregnancy. Similarly, mean +/- SEM maternal plasma CRF-BP levels in women at term out of labour were lowest at 40 weeks (3.57 +/- 0.22 nmol/l). In these women, mean +/- SEM CRF-BP levels in cord plasma (37 weeks: 6.47 +/- 0.47; 38 weeks: 6.95 +/- 1.16; 40 weeks: 5.85 +/- 0.65 nmol/l) were significantly higher than in maternal plasma at the same gestational age (37 weeks: 4.29 +/- 0.2; 38 weeks: 4.35 +/- 0.205; 40 weeks: 3.57 +/- 0.22 nmol/l). Mean +/- SEM levels of cord blood collected at delivery at term (4.93 +/- 0.14 nmol/l) showed lower CRF-BP levels than in women out of labour (6.18 +/- 0.55 nmol/l). Patients with preterm labour, with delivery within 48 h, showed significantly lower levels of cord plasma CRF-BP (4.21 +/- 0.29 nmol/l) than women at term out of labour (6.18 +/- 0.55 nmol/l) and than those at term with labour (4.93 +/- 0.14 nmol/l). Cord plasma CRF-BP levels decreased in the last 5 weeks of pregnancy, similar to maternal plasma CRF-BP levels, the lowest values resulting in women at labour or with preterm labour, thus suggesting that changes of CRF-BP in cord plasma are associated with the events of parturition.
Placenta
PMID:Cord plasma corticotropin-releasing factor-binding protein (CRF-BP) in term and preterm labour. 908 71

Chloride transport mechanisms in isolated plasma membrane vesicles were studied to characterize pathways for transcellular transport of chloride. Microvillous membrane (MVM) and basal membranes (BM) vesicles were isolated from term placentae. Western blot analysis of the anion exchanger isoform 1 (AE1) demonstrated that the density of AE1 was 12-fold higher on the MVM compared to the BM. At 30 sec, the Cl- uptake in the absence of a potential difference (p.d.) was 457.3 +/- 69.7 and 111.0 +/- 29.1 pmol/mg protein in MVM and BM, respectively (mean +/- SEM, n=6). Chloride transport pathways were characterized using diisothiocyano-2'2-disulphonic stilbene. (DIDS, 0.1 mM) and diphenylamine-2-carboxylate (DPC, 0.5 mM) in the absence or presence of inside positive membrane potentials. Anion exchange (DIDS-sensitive uptake at zero mV) was found in the MVM only. Both MVM and BM showed increased chloride uptake in the presence of inside positive potentials, suggesting the presence of chloride conductance pathways. The chloride uptake with a 25-mV inside positive p.d. could be inhibited by both DIDS and DPC in MVM and BM. However greater potentials (50 mV) showed no significant inhibition by DIDS or DPC in BM. In conclusion, the anion exchanger is unlikely to contribute significantly to chloride fluxes across BM. The data also suggest the presence of Cl- conductance pathways in both the MVM and BM which are sensitive to both DIDS and DPC.
Placenta 1998 May
PMID:Mechanisms of chloride transport across the syncytiotrophoblast basal membrane in the human placenta. 963 28

The expression of platelet-derived growth factor-A (PDGF-A) mRNA was examined in the cotyledons of normal human placentae and those from patients with pre-eclampsia. These patients exhibited pre-delivery blood pressure of 154+/-4/99+/-4 mmHg (mean+/-SEM) and met the criteria established for pre-eclampsia. During labour they received MgSO4 infusion for various time intervals (4-25 h). The PDGF-A message was quantitated to beta-actin by the solution hybridization nuclease protection assay. Since the two groups differed in two parameters (pre-eclampsia and MgSO4 treatment), the direct comparison was not feasible. An analysis of covariance revealed a significant difference in the message between the pre-eclamptic and control groups (P<0.01); the gestational age was not a significant covariate for either group but the time on MgSO4 in pre-eclampsia group was significant (P<0.002). A linear regression analysis of PDGF-A mRNA values for the pre-eclamptic group showed a time-dependent downregulation of the message by MgSO4 (P<0.01, r=- 0.796). These results show a uniform expression of PDGF-A mRNA in cotyledons of normal human placenta between 35 and 40 weeks of gestation. Furthermore, MgSO4 has an inhibitory effect on the expression of this message which may have aside from its anticonvulsive action beneficial effect on the function of pre-eclamptic placenta.
Placenta
PMID:Expression of platelet-derived growth factor-A mRNA in human placenta: effect of magnesium infusion in pre-eclampsia. 969 64


1 2 3 Next >>