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 47,337 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mast cell mediators are known to contribute to the pathogenesis of asthma. There is some disagreement concerning the numbers of mast cells in asthmatic mucosa. In this study a standardized bronchial brush technique was developed and used to assess intraepithelial mast cells and other inflammatory cells in allergic and nonallergic asthmatic and nonasthmatic subjects. A total of 10 nonasthmatic (5 allergic) and 13 asthmatic (8 allergic) subjects with stable controlled asthma treated with beta-agonist only were assessed by history, spirometry, allergy prick tests, and methacholine airway responsiveness. During fiberoptic bronchoscopy, bronchoalveolar lavage (BAL) was performed from the middle lobe and standardized bronchial brushings were taken from the lingula and left lower lobe bronchi. Quantitative cell counts were performed blind to the clinical characteristics of the subjects. The average total cell recovery from the brushings was 1.04 (SEM 0.09) x 10(6) ml, with a cell viability of 64% (5.3%). Reproducible total cell and mast cell counts were obtained from brushings taken from two lobar bronchi (ICC 0.86). Mast cells were significantly elevated in asthmatic compared with nonasthmatic subjects (1.5 +/- 0.34 versus 0.15 +/- 0.06%). Allergic asthmatic subjects had the greatest numbers of mast cells (1.86 +/- 0.48%); however, the numbers present in brushings from nonallergic asthmatic subjects were also increased (1.03 +/- 0.45%). The mast cells had the staining characteristics of mucosal mast cells, with formalin-blockable metachromatic staining and positive staining for tryptase. Both asthmatic groups also had elevated BAL eosinophils, and neutrophils were elevated in nonallergic asthmatic subjects.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Intraepithelial mast cells in allergic and nonallergic asthma. Assessment using bronchial brushings. 831 19

The purpose of this study was to evaluate the reliability of Tekdyne hand dynamometer in a controlled loading environment using the Instron 1331. Test-retest reliability, the intertool reliability of three different Tekdyne hand dynamometers, and the effects of surface area and the configuration of the forces applied to the Tekdyne hand dynamometer were studied. In addition, intertool reliability between a Jamar dynamometer modified with foam padding and the same Jamar dynamometer without padding was calculated to explore this device as an alternative measurement device. Intratool and intertool reliabilities of the three Tekdyne tools were high (ICC = 0.993-10.998, SEM = 0.106-0.045 psi and ICC = 0.995, SEM = 0.080 psi, respectively) when tested on the Instron. Both the surface area and the configuration of the force applied to the Tekdyne dynamometer appeared to influence the output reading of this device. The measurements obtained on the Tekdyne hand dynamometer correlated well with those obtained with the Jamar hand dynamometer when a controlled load was applied (r = 0.988). The Tekdyne hand dynamometer is a reliable tool in a controlled loading environment; however, further study is needed to determine its validity with respect to the Jamar dynamometer for testing human grip strength.
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PMID:The reliability and validity of the Tekdyne hand dynamometer: Part I. 866 33

The purpose of this study was to evaluate the reliability and validity of the Tekdyne hand dynamometer for measuring grip strength as compared with the Jamar dynamometer. Additionally, it was hypothesized that one to six weeks after carpal tunnel release (CTR) grip strength would appear to be greater when tested on a softer tool than when tested on the Jamar dynamometer. The Tekdyne, the standard Jamar instrument (SJ) and a Jamar dynamometer modified with foam padding (MJ) were compared in 48 subjects without upper-extremity abnormality and 30 subjects following open-palm CTR. The Tekdyne and the Jamar hand dynamometers were well correlated in the presence and in the absence of upper-extremity abnormality (r = 0.975 and r = 0.871, respectively). Tekdyne intrasession reliabilities in both the non-surgical group and the surgical group were high (ICC = 0.954, SEM = 0.290 psi and ICC = 0.958, SEM = 0.219 psi). The Tekdyne intersession reliability of the non-surgical group was high (ICC = 0.971, SEM = 0.22 psi). There was no statistically significant difference between the ratios of the non-operated grip strengths across the three measurement devices, suggesting that the softer device did not promote greater force production by the operated hand.
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PMID:The reliability and validity of the Tekdyne hand dynamometer: Part II. 866 34

The importance of accurately reproducing isokinetic strength values is critical for the assessment of training induced changes in muscle function. The purpose of this study was to determine the test re-test reliability of the Biodex System 2 Isokinetic Dynamometer for concentric quadricep and hamstring strength and muscular endurance. Twenty-one healthy volunteers underwent isokinetic testing on 2 occasions separated by 7 days. Each subject performed 5 repetitions at 60 degrees/ second followed by a muscular endurance test which consisted of 30 repetitions at 180 degrees/second. The results demonstrated high ICC values ranging from r = 0.88 to r = 0.97 and r = 0.82 to r = 0.96 for variables measured at 60 degrees/second and 180 degrees/second, respectively, SEM values were found to range from low to moderate, 4.8% to 11.6% and 5.6% to 10.8%, at 60 and 180 degrees/second, respectively. ICC coefficients were found to be low, r = 0.52 to r = 0.74, and SEM values were found to be high, 9.8% to 20.8%, for the work fatigue index at 180 degrees/second. The results demonstrate that isokinetic values obtained at 60 and 180 degrees/second are highly reproducible with the Biodex System 2 Dynamometer provided that adequate calibration, gravity correction and patient positioning is recorded and standardized. However, the work fatigue index as a measure of muscular endurance has not been shown to be reliable or precise. Changes in muscular strength over time should be considered valid if these differences lie beyond the SEM values reported in this study.
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PMID:Reliability and precision of isokinetic strength and muscular endurance for the quadriceps and hamstrings. 908 Dec 67

Clough et al. (1997) reported that 95% of lactate values obtained using an Accusport analyser may be up to 2.6 mM below or 2.1 mM above YSI 2300 analyser values over the range 0-16 mM. This variability is substantial and unsuitable for research purposes, The objectives of this study were to re-examine the specific validity and reliability of an Accusport analyser and to develop a regression equation to improve the accuracy of Accusport measurements. Duplicate measurements of lactate concentration were made on both an Accusport (Boehringer Mannheim) and Analox LM3 Multi Channel analyser on 17 blood samples taken from two subjects performing a discontinuous incremental exercise protocol. Analysis of duplicate measurements revealed good test-retest reliability for Accusport (TEM 0.35 mM; SEM 0.24 mM; ICC r = 0.995) and Analox (TEM 0.07 mM; SEM 0.09 mM; ICC r = 0.999). The mean values for duplicate samples recorded on both the Accusport and Analox between the lactate range of 1-13 mM revealed an average difference between the two analysers of 1.7 mM (P< 0.01, range 1.0-2.9 mM) but values demonstrated a high level of association (ICC r = 0.853; P< 0.05). The level of agreement indicated that in 95% of cases the differences would lie between + 0.5 to + 3.0 mM with the Accusport values always higher than Analox. Linear regression analysis calculated the following equation to predict Analox values from Accusport values: Analox = -0.749 + 0.837Accusport (R2 = 0.990). The results showed the portable Accusport analyser to be reliable and it demonstrated good association with Analox LM3 lactate analyser measures. However, a need exists to develop specifically generated regressions from Accusport and Analox LM3 analyser measures to provide more accurate results when interpreting lactate values from Accusport measures taken in the field.
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PMID:Examination of the validity and reliability of the Accusport blood lactate analyser. 1133 89

The forced expiratory volume in 1 sec (FEV1) is the most established outcome measure in chronic obstructive pulmonary disease (COPD). However, changes in FEV1 in response to treatment are small in relation to the repeatability of the measurement and there is increasing interest in other measures including markers of lower airway inflammation in induced sputum, assessment of symptoms and health status using visual analogue scores, and questionnaires. Little is known about the repeatability of these measures or the safety of sputum induction in COPD. We have assessed the safety of sputum induction in 61 subjects with moderate and severe COPD who participated in a placebo-controlled cross-over study The within-subject repeatability of sputum markers of airway inflammation, health status using the chronic respiratory disease questionnaire (CRQ) and symptom visual analogue scores (VAS) were estimated from the data obtained from before and after 2 weeks of treatment with placebo. Sputum induction was performed on 122 occasions and was successful resulting in a cytospin adequate to assess a differential cell count in 95% of inductions. The group mean (SEM) FEV1 was 1.09 (0.05)[41.6 (2.9)% predicted] and the mean (SEM) fall in FEV1 after sputum induction was 120 ml (6) and % fall 10.9% (0.55%). Seven inductions were stopped due to a fall in FEV1 >20% and at a further 13 visits the full sputum induction protocol was not completed due to development of symptoms. The reproducibility of measurements, calculated by the intra-class correlation coefficient, was relatively high for all indices measured (0.4-0.95) with the exception ofthe proportion of lymphocytes (0.15) and epithelial cells (0.3). The ICC for symptom scores and the CRQ domains ranged between 0.87 and 0.96. In conclusion, sputum induction is safe and the cell and fluid phase mediators repeatable in the investigation of airway inflammation in patients with COPD. VAS symptom scores and the CRQ are reproducible outcome measures in COPD.
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PMID:Induced sputum and other outcome measures in chronic obstructive pulmonary disease: safety and repeatability. 1177 99

Use of maximal aerobic power (VO2(max)) testing, which requires subjects to exercise to physiological limits, may deter eligible candidates from volunteering for trials and may also be contraindicated in patients suffering from various medical illnesses. An alternative to maximal testing is submaximal testing. The Aerobic Power Index, which represents the aerobic component of the Tri-level Fitness Profile, is a submaximal test that has been shown to be reliable in trained athletes. The purpose of this study was to establish reliability of the Aerobic Power Index, as well as associated variables of VO2 (ml x kg(-1) x min(-1) and rate of perceived exertion (RPE), in a group of sedentary subjects. Results for the 20 subjects who participated in a test-retest trial indicated high reliability (ICC r = 0.98, %TEM 3.87 W x kg(-1); SEM 0.04 W x kg(-1) for the main measurement outcome of Watts per kilogram (W x kg(-1)). Oxygen uptake (ml x kg(-1)min(-1)) also demonstrated high reliability (ICC r = 0.92; %TEM 4.63 ml x kg(-1) x min(-1); SEM 0.58 ml x kg(-1) min (-1), as did RPE (ICC r = 0.97,%TEM 7.78; SEM 0.63). Limits of agreement were -0.02+/-0.16 W x kg(-1). -0.41+/-2.31 ml x kg(-1) x min(-1) for VO2 and -0.05 < or = 2.10 for RPE. These results indicate that the Aerobic Power Index is a reliable submaximal exercise test for use in sedentary subjects.
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PMID:Test-retest reliability of the aerobic power index component of the tri-level fitness profile in a sedentary population. 1472 94

Use of a shank shell has been shown to estimate tibial transverse rotations better than skin-mounted markers. However, the day-to-day reliability of the transverse tibial rotations using an individually molded shank shell has not been previously investigated. This study examined the between-tests and trials reliability of an individually molded shank shell for measuring peak tibial internal and external rotations, time of peak values, and tibia range of motion during 5 walking trials. The trial-to-trial reliability of tibial transverse rotations was measured in 14 healthy individuals while the test-retest reliability was measured in 10 persons on two occasions. Trial-to-trial reliability for peak transverse rotations, time of peak values, and tibia range of motion ranged from ICC (3,1) 0.59-0.95. The PCA between trials showed that 88-99 % of values were within 3 degrees of agreement. Test-retest reliability for peak rotations, tibia range of motion, and time of peak values ranged from ICC (3,1) 0.70-0.89 with SEM 1.6-2.21 degrees , 0.021 %, and 0.034 %, respectively. The PCA between tests showed that 70-100 % of values were within 3 degrees of agreement. The use of an individually molded shell and the close attachment of the shank shell to the individual's shank resulted in reliable test-retest and trial-to-trial data.
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PMID:Reliability of an individually molded shank shell for measuring tibial transverse rotations during the stance phase of walking. 1608 20

We examined the relationship between anterior knee laxity (AKL), evaluated while the knee was nonweight bearing, and anterior translation of the tibia relative to the femur (ATT), evaluated when the knee transitioned from nonweight-bearing to weight-bearing conditions in response to an applied compressive load at the foot. Twenty subjects with normal knees (10 M, 10 F; 25.2 +/- 4.1 years, 169.8 +/- 11.5 cm, 71.6 +/- 16.9 kg) underwent measurements of AKL and ATT of the right knee on 2 days. AKL was measured at 133N with the KT-2000. ATT was measured with the Vermont Knee Laxity Device and electromagnetic position sensors attached to the patella and the anteromedial aspect of the proximal tibia. Three trials for each measure were averaged and analyzed. Measurement consistency was high for both AKL (ICC = 0.97; SEM = 0.44 mm) and ATT (ICC = 0.88; SEM = 0.84 mm). Linear regression revealed that AKL predicted 35.5% of the variance in ATT (p = 0.006), with a prediction equation of Y(ATT) = 3.20 + 0.543(X(AKL)). Our findings suggest that increased AKL is associated with increased ATT as the knee transitions from nonweight-bearing to weight-bearing conditions. The potential for increased knee joint laxity to disrupt normal knee biomechanics during activities such as landing from a jump, or the foot strike phase of gait deserves further study.
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PMID:Nonweight-bearing anterior knee laxity is related to anterior tibial translation during transition from nonweight bearing to weight bearing. 1645 28

This investigation was designed to test the validity and reliability of a new measure of inversion/eversion ankle stiffness on a unique medial/lateral swaying cradle device utilizing a test/retest with comparison to a known standard. Ankle stiffness is essential to maintaining joint stability. Most ankle injuries occur via an inversion mechanism. To date, very little information is available regarding stiffness of the evertor muscles in the prevention of excessive inversion joint rotation. Transient oscillation data representing inversion/eversion stiffness was obtained in a bipedal weight-bearing stance with an upright posture. Using commercially available springs with stiffness of 4.80N/cm the measured value recorded by the cradle was 4.87N/cm. Mean active stiffness values of the ankle were 35.70Nm/cm (SD 9.45). The trial-to-trial reliability ICC (2,1) coefficient was 0.96 with an SEM of 2.05Nm/rad, and the day-to-day reliability ICC (2,k) coefficient was 0.93 and an SEM of 3.00Nm/rad. The results demonstrate that inversion/eversion ankle stiffness measures on this device are a valid, repeatable and consistent measure. This is relevant because the ability to accurately quantify inversion/eversion ankle stiffness will improve our understanding of biomechanical stability and factors that influence it. It will also enable identification of ankle injury risk factors that will lead to more efficient rehabilitation programs and injury prevention strategies.
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PMID:Validity and reliability of a new in vivo ankle stiffness measurement device. 1645


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