Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0406810 (NAME)
 13,345 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of the present study was to investigate the involvement of nitric oxide and prostaglandin pathways in the cardioprotective actions mediated by bradykinin via the combined inhibition of angiotensin converting enzyme and aminopeptidase P in an in vivo rat model of acute ischemia (30 min) and reperfusion (4 h). Myocardial infarction was produced by occlusion of the left anterior descending coronary artery for 30 min followed by 4 h of reperfusion. Infarct size was measured by using the staining agent TTC (2,3,5-triphenyl-tetrazolium chloride). Lipid peroxide levels in serum and in heart tissue were estimated spectrophotometrically. A lead II ECG was monitored at various intervals throughout the experiment. Infarct size expressed as percent of left ventricle was found to be 50.5 +/- 3.5 in control animals and was reduced to 19.4 +/- 1.1 and 15.0 +/- 2.1 with the combined treatment of enalapril or lisinopril and 2-mercaptoethanol, respectively. There was no significant difference in the infarct size of control animals and in the animals treated with HOE140 prior to the combined treatment. Infarct size reduction obtained with the combined inhibition with enalapril and 2-mercaptoethanol or lisinopril and 2-mercaptoethanol was blocked partially but significantly with the prior administration of L-NAME (Nomega-nitro-L-arginine methyl ester) or aspirin, suggesting the involvement of both nitric oxide and prostaglandin pathways in the cardioprotective actions mediated by bradykinin.
Pol J Pharmacol
PMID:Nitric oxide- and prostaglandin-mediated cardioprotection by bradykinin in myocardial ischemia and reperfusion injury. 1473 97

A number of epidemiological studies have demonstrated that moderate red wine consumption significantly decreases the risk of ischemic heart disease. Our earlier studies provide evidence that Italian red wine modulates primary hemostasis and prevents experimental venous thrombosis in rats, independently of its alcohol content, by a nitric oxide (NO)-mediated mechanism. In the present study, we have tested whether Bulgarian red and white wines can influence thrombotic process and primary hemostasis in rats. NO and PGI2 were evaluated as possible mediators of these effects. We have found that red wine treatment (for 10 days) induced a marked prolongation of bleeding time, decrease in platelet adhesion to fibrillar collagen, reduction in venous thrombus weight and shortening of occlusion time in arterial thrombosis model. The fall in venous thrombus weight was also observed after white wine supplementation. Red wine affects hemostasis and venous thrombosis after its iv injection 15 min before experiment. These effects were prevented by NO inhibitor (L-NAME) and PGI2 inhibitor (indomethacin). Our results demonstrate the ability of Bulgarian wines to modulate primary hemostasis and prevent venous and arterial thrombosis in rat.
Pol J Pharmacol
PMID:Effects of Bulgarian red and white wines on primary hemostasis and experimental thrombosis in rats. 1473 Jan 5

The aim of the present study was to investigate the influence of nitric oxide (NO) on the contractile activity of the isolated porcine ovarian and uterine arteries. Segments of the vessels, obtained from the pigs on days 1-5, 8-13 and 17-20 of the oestrous cycle, were mounted in the organ bath with Krebs-Ringer solution and contractile activity changes of the vessels were measured using isometric transducers. In Experiment I the arteries pretreated with norepinephrine (NE; 10(-7) M) were treated with sodium nitroprusside (SNP, 10(-8)-10(-4) M), a NO donor. In Experiment II administration of NE (10(-7) M) was preceded by treatment with Nomega-nitro-L-arginine methyl ester (L-NAME, 10(-8)-10(-6) M), an inhibitor of NO synthase. Donor of NO at doses of 10(-8)-10(-7) M did not affect (P>0.05) the contractility, while at doses of 10(-5)-10(-4) M caused a dose-dependent relaxation (P<0.05) of both ovarian and uterine arteries in all periods examined. Moreover, SNP at doses of 10(-6)-10(-4) M it caused significantly higher (P<0.05) relaxation of the ovarian arteries collected on days 8-13 as compared to the vessels from days 1-5 of the cycle. Pretreatment of the vessels with L-NAME caused a dose-dependent, significant (P<0.05) increase in the vasocontractile action of NE in both the ovarian and uterine arteries as compared to contractile activity of NE administered alone. Moreover, L-NAME pretreatment at a dose of 10(-6) M caused significantly higher (P<0.05) intensification of NE action in ovarian and uterine arteries collected on days 8-13 as compared to the vessels from days 1-5 (P<0.05) and 17-20 (P<0.05) of the oestrous cycle. Obtained results indicate that NO plays an important role in the regulation of the contractile activity of the isolated porcine ovarian and uterine arteries. Our data suggest that this action may be, at least in a part, dependent on the hormonal status of the organism.
Pol J Vet Sci 2004
PMID:The influence of nitric oxide on the contractile activity of the isolated porcine ovarian and uterine arteries. 1523 May 37

The aim of this study was to investigate whether endogenous superoxide anion is involved in the regulation of renal Na(+),K(+)-ATPase and ouabain-sensitive H(+),K(+)-ATPase activities. The study was performed in male Wistar rats. Compounds modulating superoxide anion concentration were infused under general anaesthesia into the abdominal aorta proximally to the renal arteries. The activity of ATPases was assayed in isolated microsomal fraction. We found that infusion of a superoxide anion-generating mixture, xanthine oxidase (1 mU/min per kg) + hypoxanthine (0.2 mumol/min per kg), increased the medullary Na(+),K(+)-ATPase activity by 49.5% but had no effect on cortical Na(+),K(+)-ATPase and either cortical or medullary ouabain-sensitive H(+),K(+)-ATPase. This effect was reproduced by elevating endogenous superoxide anion with a superoxide dismutase inhibitor, diethylthiocarbamate. In contrast, a superoxide dismutase mimetic, TEMPOL, decreased the medullary Na(+),K(+)-ATPase activity. The inhibitory effect of TEMPOL was abolished by inhibitors of nitric oxide synthase (L-NAME), soluble guanylate cyclase (ODQ) and protein kinase G (KT5823). The stimulatory effect of diethylthiocarbamate was not observed in animals pretreated with a synthetic cGMP analogue, 8-bromo-cGMP. An inhibitor of NAD(P)H oxidase, apocynin (1 mumol/min per kg), decreased the Na(+),K(+)-ATPase activity in the renal medulla and its effect was prevented by L-NAME, ODQ or KT5823. In contrast, a xanthine oxidase inhibitor, oxypurinol, administered at the same dose was without effect. These data suggest that NAD(P)H oxidase-derived superoxide anion increases Na(+),K(+)-ATPase activity in the renal medulla by reducing the availability of NO. Excessive intrarenal generation of superoxide anion may upregulate medullary Na(+),K(+)-ATPase leading to sodium retention and blood pressure elevation.
Acta Biochim Pol 2004
PMID:Nitric oxide -- superoxide cooperation in the regulation of renal Na(+),K(+)-ATPase. 1562 65

The influence of nitric oxide (NO) on hypnotic activity of diazepam, chlordiazepoxide and clonazepam was studied in mice. Administration of both non-selective NO synthase inhibitors: N(G)-nitro-L-arginine methyl ester (L-NAME), N(G)-nitro-L-arginine (L-NOARG) and selective NO synthase inhibitor 7-nitroindazole (7-NI) resulted in significant increase in the duration of diazepam-, chlordiazepoxide- and clonazepam-induced sleep. The effects of co-administration of the examined inhibitors with benzodiazepines were not changed by L-arginine, a substrate for NO formation. Administration of L-arginine alone had no effect on the duration of sleep induced by benzodiazepines. Methylene blue, the guanyl cyclase inhibitor, was able to increase the duration of benzodiazepine-induced sleep. These findings suggest that the cGMP/NO system may participate in hypnotic effects of benzodiazepines.
Pol J Pharmacol
PMID:Involvement of nitricoxidergic system in the hypnotic effects of benzodiazepines in mice. 1566 84

The aim of this study was to investigate the role of nitric oxide (NO) in the regulation of blood flow in the porcine uterine artery during the course of the oestrous cycle. Experiments were carried out on animals on days 1-5, 8-13 and 17-20 of the oestrous cycle. After induction of anesthesia and opening of the abdominal cavity, blood samples were collected from the ovarian and uterine arteries and veins to determine nitrate and nitrite concentrations; on the opposite side to the blood sampling the branch of the uterine artery was prepared and a venous catheter was inserted into the artery. For measuring the changes in the blood pressure the catheter was connected via a polyvinyl cannula to a pressure transducer. Sodium nitroprusside (NP; 2.4 microg, 24 microg and 240 microg; a NO donor) or Nomega-nitro-L-arginine methyl ester (L-NAME; 2.2 microg, 22 microg, 220 microg and 2200 microg; an irreversible inhibitor of neuronal and endothelial NO synthase and reversible inhibitor of macrophage NO synthase) was administered via a bolus into the uterine artery. Nitrite/nitrate concentrations were: higher (P < 0.05) in the uterine vein as compared to the uterine artery on days 1-5 of the oestrous cycle; lower (P < 0.05) in the uterine artery as compared to the ovarian and uterine veins as well as in the ovarian artery as compared to the ovarian vein on days 8-13 of the cycle; lower (P < 0.05) in the uterine artery as compared to the ovarian artery and uterine and ovarian veins on days 17-20. Administration of NP at doses of 2.4 microg and 24 microg and L-NAME at all doses examined did not affect (P > 0.05) the blood pressure in the uterine artery in all periods examined. NP at a dose of 240 microg decreased (P < 0.001) the blood pressure in the arteries in all periods examined as compared to blood pressure before NP treatment. The results obtained indicate that NO is involved in the regulation of blood flow through the porcine reproductive tract. Moreover, our results suggest that the action of NP in the porcine uterine artery is not dependent on the phase of the oestrous cycle.
Pol J Vet Sci 2005
PMID:Influence of nitric oxide on the blood flow in the porcine uterine artery; an in vivo study. 1618 May 80

Pituitary adenomas are common benign neoplasms, accounting for approximately 15% of intracranial tumors. In systematic autopsy, pituitary tumors are found in 25%, of the population, but only one-third of these tumors give rise to clinical manifestations. Why most of these neoplasms remain undiagnosed and pituitary carcinomas are extremely rare? The progress in the studies concerning pituitary tumorigenesis is rather slow and, due to several limitations, including the anatomic inaccessibility of human pituitary gland, the lack of functional human cell lines in culture and the discrepancies between human and animal pituitary oncogenesis (in rodents pituitary hyperplasia is a prerequisite for adenoma development). In humans, the majority of pituitary tumors are monoclonal in origin and derived from single mutated pituicyte, rarely hyperplasia is a prerequisite for adenoma formation. As in the case of other tumors, activating mutations in oncogenes (GNAS1, PTTG) and inactivating mutations in tumor suppressor genes (MEN1, CNC1) lead to pituitary tumors development. However, mutations in classic oncogenes are very rarely associated with these tumors. Moreover, the important role of some hypothalamic hormones, peripheral hormones and their receptors (e.g. GHRH, dopamine D2 receptor, PRL receptor, estrogens, thyroid hormone receptor) and growth factors (e.g. FGF, EGF, TGF) is postulated and partially proved in promotion of pituitary tumorigenesis. Further studies are required to determine which of these events are truly primary changes in pituitary tumorigenesis, what may allow development of gene therapy.
Endokrynol Pol
PMID:[Molecular aspects of pituitary tumors]. 1635 Jul 28

The aim of our study is to introduce a larger number of doctors to the subject of lentigines. They may be a first syndrome coexistent with very rare multiple organ defects as syndrome Peutz-Jeghers, LEOPARD, LAMB and Carney syndrome.
Pol Merkur Lekarski 2009 Jan
PMID:[Lentigines in different multiple organ defects syndromes]. 1939 16

Primary pigmented nodular adrenocortical disease (PPNAD) is a rare adrenal tumour causing corticotrophin-independent Cushing's syndrome. It occurs mainly in children and young adults. The histological examination is characterised by small pigmented micronodules on the adrenal cortex. The diagnosis is most often seen in patients with Carney Complex, but it can also occur in isolation. We report a case of Carney Complex that was referred for adrenalectomy. The procedure was uneventful and the patient was well at discharge. The adrenal pathology showed numerous black nodules measuring less than 2mm in diameter. This feature was pathognomonic of primary pigmented nodular adrenocortical disease.
Endokrynol Pol 2011
PMID:Primary pigmented nodular adrenocortical disease. 2171 12

Obstructive cholestasis is associated with overproduction of endogenous opioids, nitric oxide (NO) and cytokines in the blood stream. Nitro-L-arginine methyl ester (L-NAME) administration decreases the NO serum level and it is able to reduce related complications. The aim of this research is to survey the effects of the NO inhibitor on complications relating to cholestasis in liver cells and intrahepatic biliary ducts. We used five groups of animals: control, sham-operated (surgical control), bile duct ligated (BDL) group, BDL and normal saline infused group, and BDL with L-NAME administrated group. After 3 weeks all animals were killed, histopathology of liver cells and intrahepatic biliary ducts were evaluated by hematoxylin-eosin (HE), PAS (periodic acid-Schiff) and trichrome staining. The status of inflammation and fibrosis was evaluated by the modified Knodell score system. Microscopic study of different groups showed that the necro-inflammatory score in the control group was 0.36, it was 1 in the sham-operated group and it raised to 15.2 in the cholestatic group. After administration of L-NAME it had a meaningful decrease to 7, but in the saline-treated group, the score was 16. L-NAME with the mentioned dose was capable of decreasing the serum nitric oxide level, although it is able to decrease the unfavorable complications of cholestatic jaundice.
Pol J Pathol 2012 Dec
PMID:Effects of nitric oxide synthase inhibitor (L-NAME) on cytopathologic changes due to cholestasis in hepatic cells of adult male rats. 2335 93


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