Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0393754 (
HSA
)
2,996
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
CopA is a Cu(I)-exporting transmembrane P
1B
-type
ATPase
from Bacillus subtilis. It contains two N-terminal cytoplasmic domains, CopAab, which bind Cu(I) with high affinity and to form higher-order complexes with multiple Cu(I) ions. To determine the precise nature of these species, electrospray ionisation mass spectrometry (ESI-MS) under non-denaturing conditions was employed. Up to 1 Cu per CopAab resulted in Cu coordination to one or both CopAab domains. At >1 Cu/CopAab, two distinct dimeric charge state envelopes were observed, corresponding to distinct conformations, each with Cu
6
(CopAab)
2
as its major form. The influence of the physiologically relevant low molecular weight thiol bacillithiol (
BSH
) on Cu(I)-binding to CopAab was assessed. Dimeric CopAab persisted in the presence of
BSH
, with previously undetected Cu
7
(CopAab)
2
and Cu
6
(CopAab)
2
(
BSH
) forms apparent.
...
PMID:Mass spectrometric studies of Cu(I)-binding to the N-terminal domains of B. subtilis CopA and influence of bacillithiol. 3034 52
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