Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0393754 (HSA)
 2,996 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Priming of mice with a conjugate of HSA with sheep red cells induced a high level of memory to HSA, with very little antibody production ("pure priming"). HSA specific antigen binding cells in the spleens of the primed mice were assayed by means of a rosette technique, using HSA conjugated to donkey red cells. Rosette formation was almost completely inhibited by soluble HSA, thus confirming that the RFC were specific for this antigen. Spleens of primed mice contained up to 0.6% RFC, as compared to 0.08% HSA specific RFC in the spleens of non immunized animals. Suspensions enriched in rosettes (containing up to 16% RFC) were prepared by centrifugation on BSA density gradients. Adoptive transfer experiments showed that the rosette rich fraction contained all the memory cells. A marginal level of memory could be transferred to irradiated recipients with 3000 rosettes. A comparable degree of responsiveness to HSA could also be transferred with 70,000 RFC enriched from spleens of non immunized mice, but only when injected together with primed, RFC depleted spleen cells. Kinetic studies showed that the level of memory correlated well with the number of RFC up to two months after priming. The number of RFC decreased at later time intervals (though remaining higher than in controls at all times), without a corresponding decrease in the level of memory. A change in the quality of the memory cell with time is postulated.
 ...
PMID:Relation of antigen-binding cells to immunological memory. 126 74

A correlation between spleen B-cell antibody production against MAG antigens and the presence of different antibodies in circulation or antibodies bound to target glands was attempted. The number of 7S and 19S Ig-secreting cells (ISC) found in the spleen and the number of ISC generated after in vitro stimulation of the cells with MAG antigens were evaluated by using the hemolytic plaque assay. Low numbers of 7S and 19S ISC--less than 0.01% of spleen cells--were generated in response to MAG immunization, and no significative increase was observed after in vitro culture of spleen cells with MAG antigens, suggesting that secretory activity of the B-cells can not be improved when liberated from humoral homeostatic mechanisms. The humoral response of MAG-immunized rats, investigated by complement fixation and immunodiffusion assays, has proved negative, and in only two out of 17 rats a weak haemagglutinating activity was observed. Attempts to detect antibodies bound to cellular MAG antigens by immunofluorescence have shown a weak fluorescence in the epithelial cells of the prostate gland in only two rats. In both cases a concomitant tissue damage was observed, but in nine out of 11 cases with histological alterations no fluorescence was observed in the target glands. The medium value of rosette-forming cells (RFC) found in the spleen of MAG-immunized rats did not significantly differ from the value of the HSA-treated control group, although both groups differ in their specific humoral response.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:Experimental autoimmunity to rat male accessory glands (MAG): circulating antibodies, immunoglobulins bound to target glands, and immunoglobulins-secreting cells. 398 55