Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0393754 (
HSA
)
2,996
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
[VO(acac)
2
] is a remarkable vanadium compound and has potential as a therapeutic drug. It is important to clarify how it is transported in blood, but the reports addressing its binding to serum proteins have been contradictory. We use several spectroscopic and mass spectrometric techniques (ESI and MALDI-
TOF
), small-angle X-ray scattering and size exclusion chromatography (SEC) to characterize solutions containing [VO(acac)
2
] and either human serum apotransferrin (apoHTF) or albumin (
HSA
). DFT and modeling protein calculations are carried out to disclose the type of binding to apoHTF. The measured circular dichroism spectra, SEC and MALDI-
TOF
data clearly prove that at least two VO-acac moieties may bind to apoHTF, most probably forming [V
IV
O(acac)(apoHTF)] complexes with residues of the HTF binding sites. No indication of binding of [VO(acac)
2
] to
HSA
is obtained. We conclude that V
IV
O-acac species may be transported in blood by transferrin. At very low complex concentrations speciation calculations suggest that [(VO)(apoHTF)] species form.
...
PMID:Interaction of [V
IV
O(acac)
2
] with Human Serum Transferrin and Albumin. 2865 Oct 41
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