Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0393754 (HSA)
 2,996 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of prior injection of increasing doses of dinitrophenylated bovine serum albumin (DNP-BSA) mixed in Freund's complete (FCA) or incomplete (IFA) adjuvants on the antibody response to a subsequent challenge with an optimal immunizing dose of DNP-BSA in FCA has been examined and compared with the effect of competition with the unrelated antigen bovine gamma-globulin (BGG). An unresponsive state was induced by pretreatments with either a minimal dose of antigen, a large antigen excess or a competitive mixture in FCA. The specificity of this unresponsiveness was ascertained by the normal response of pretreated animals to the unrelated antigen arsanilic-human albumin (ARS-HSA). Prior injection of either a minimal dose (0,2 mug), an antigen excess (20 mg) of DNP-BSA or an optimal dose (20 mug) of DNP-BSA mixed with an excess of BGG, incorporated in IFA, also induced a specific tolerance to DNP-BSA. The former treatment induced a low zone tolerance whereas both later treatments induced a high zone tolerance.
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PMID:Induction of tolerance in adult guinea-pigs by two antigen dosage levels and by antigenic competition. 5 74

The effect of related and unrelated compounds on the specific binding of dinitrophenyl-coupled bacteriophage (DNP-T4) to lymphoid cell receptors has been examined and compared with the effect on the neutralization of DNP-T4 by anti-DNP serum. Spleen cells and sera from Balb/c mice immunized with DNP-bovine serum albumin were used. The binding of DNP-T4 to the cells was inhibited by DNP-eAcp, di-DNP-Lys, DNP-Tyr, DNP-p(Ornith) and DNP-BSA (among the DNP-derivatives tested), TNP-BSA, ARS-p(Tyr) and TGA. In addition with the above named DNP and TNP compounds, the DNP-T4neutralization by antiserum was also prevented by DNP-derivatives with either L-cysteic acid, alanine, glutamine or poly-L-glutamic acid, while ARS-p(Tyr) and TGA were not effective. Plain carriers (BSA, HSA, poly-ornithine, polylysine and polyglutaminc acid) and cell-mitogens (ConA, LPS and PPD) had no significant inhibitory effect. The results obtained indicate the occurrence of differences between cell-bound receptors and circulating antibodies in what concerning their specific reaction with the dinitrophenyl determinant.
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PMID:Inhibition of specific binding of DNP (dinitrophenyl) determinant to lymphoid-cell receptors by related and unrelated compounds : quantitative studies in vitro. 6 Sep 7

The administration of protein antigens into earthworms Lumbricus terrestris and Eisenia foetida induces the formation of antigen-binding protein (ABP) with the maximum response occurring between days 4 and 8. High proteolytic activities observed both in coelomocytes and in coelomic fluids cause rapid antigen degradation; the majority of antigen is digested during the first 24 h. To analyze the role of proteolytic processing of antigen in ABP response in vitro, the intact antigen (ARS-HSA) as well as its proteolytic fragments were added to tissue explant cultures and ABP formation followed by indirect ELISA using mAb to ABP. The response to small fragments was comparable to that induced by intact ARS-HSA. Furthermore, the response to intact antigen was almost completely blocked by the non-toxic serine protease inhibitor Pefabloc, while the response to small fragments was only slightly reduced. The kinetics of response to intact antigen significantly differed from that induced by small (< 3 kDa) fragments. We suggest that proteolytic processing is involved in the stimulation of ABP formation.
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PMID:Antigen processing in earthworms. 800 50